| Actin a [Q9U1E8] | |
|---|---|
| Systematic Name | LmjF.04.1230 [Leishmania major] |
| Gene Name | L1156.01 |
| Molecular Weight | 42019 Da |
| Protein Sequence Size | 376 |
| Function | |
| Charge | -6 |
| Isoelectric Point | 5.2956 pH |
| Description | Actin a. |
| Subcellular Location | actin cytoskeleton; cytoplasm[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q9U1E8|Q9U1E8_LEIMA Actin a - Leishmania major. ADNEQSSIVCDNGSGMVKAGFSGDDAPRHVFPSIVGRPKNMQAMMGSANKTVYVGDEAQS KRGVLSLKYPIEHGIVTNWDDMEKIWHHTFYNELRVNPEQHNVLLTEAPMNPKQNREKMT QIMFETFNVPSLYIGIQAVLSLYSSGRTTGIVLDAGDGVTHTVPIYEGYSLPHAVRRVDM AGRDLTEYLMKIMMETGTTFTTTAEKEIVRNVKEQLCYVALDFEEEMTNSAKSANEEAFE LPDGNVMMVGNQRFRCPEVLFKPSLIGLDEAPGFPEMVYQSINKCDIDVRRELYGNIVLS GGSTMFLNLPERLAKEISNLAPSSIKPKVVAPPERKYSVWIGGSILSSLTTFQTMWVKKS EYDESGPSIVHNKCF |
| DNA Sequence | >LmjF04.1230 |||actin|Leishmania major|chr 4|||Manual ATGGCTGACA ACGAGCAGAG CTCCATCGTC TGCGATAACG GTTCCGGCAT GGTGAAGGCCGGCTTCTCTG GCGATGACGC TCCTCGCCAC GTTTTCCCTT CGATCGTGGG CCGCCCGAAGAACATGCAGG CCATGATGGG GAGCGCGAAC AAGACGGTTT ATGTTGGCGA CGAGGCGCAGTCGAAGCGCG GCGTGCTGTC GCTGAAGTAC CCGATCGAGC ACGGAATTGT GACAAACTGGGATGACATGG AGAAGATCTG GCACCATACC TTCTACAACG AGCTGCGCGT GAACCCCGAGCAGCACAACG TGCTGTTGAC GGAAGCGCCG ATGAACCCGA AGCAGAACCG TGAGAAGATGACGCAAATCA TGTTCGAGAC GTTCAATGTG CCGTCGCTGT ACATTGGAAT TCAGGCTGTGCTGTCCCTGT ATTCCTCTGG CCGCACCACC GGTATTGTGC TTGATGCGGG CGATGGCGTGACGCACACCG TTCCAATCTA CGAGGGCTAC TCCCTGCCGC ACGCGGTGCG GCGTGTGGACATGGCTGGCC GCGACCTGAC GGAGTACCTG ATGAAGATCA TGATGGAGAC GGGTACGACGTTCACGACGA CCGCGGAGAA GGAGATTGTG CGCAACGTGA AGGAGCAGCT GTGCTACGTAGCGCTGGACT TTGAGGAGGA GATGACGAAC AGCGCCAAGT CGGCGAACGA GGAGGCGTTTGAGCTGCCGG ACGGCAACGT CATGATGGTG GGCAATCAGC GCTTCCGCTG CCCAGAGGTGCTGTTCAAGC CGTCGCTCAT TGGTCTCGAC GAGGCTCCCG GCTTCCCGGA GATGGTGTACCAGTCCATCA ATAAGTGTGA CATTGATGTG CGGCGCGAGC TGTACGGGAA CATCGTGCTGTCCGGTGGCT CGACCATGTT CCTCAACCTT CCGGAGCGCC TGGCGAAGGA GATCTCGAACCTGGCGCCGT CGTCGATAAA GCCGAAGGTG GTTGCGCCGC CGGAGCGCAA ATACAGCGTGTGGATCGGCG GCTCGATTTT GTCATCGCTG ACGACCTTCC AGACAATGTG GGTGAAGAAGAGCGAGTACG ACGAGTCTGG CCCAAGCATC GTGCACAACA AGTGCTTCTG A |
| Actin a Q9U1E8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | protein binding; structural constituent of cytoskeleton |
| Biochemical Pathway | cytoskeleton organization and biogenesis |
| Regulatory Pathway | |
| KEGG Pathways | K05692 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | cardiac muscle alpha actin proprotein [Homo sapiens] | 69 | 1e-160 | 561 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YFL039c actin | Saccharomyces cerevisiae | 71% | 1e-162 | 565 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00340 | Actins signatures | 54-64; | PS00406 | |
| PDOC00340 | Actins signatures | 357-365; | PS00432 | |
| PDOC00340 | Actins signatures | 105-117; | PS01132 | |
| Acylation | N-myristoylation site | 16-21; 75-80; 159-164; 198-203; 344-349; | PS00008 | |
| Glycosylation | N-glycosylation site | 13-16; 50-53; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 336-339; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 23-26; 78-81; 203-206; 234-237; 361-364; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 61-63; 67-69; 146-148; 231-233; 325-327; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 211-219; | PS00007 | |
| Actin a [Q9U1E8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1yagA | |
| Percent Identity | 71% | |
| Target Region | 1-376 | |
| Template Region | 4-372 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 84412-18-0 | ADENOSINE-5'-TRIPHOSPHATE | 507.181 | C10 H16 N5 O13 P3 | O=P(O)(O)OP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 1yag |
| 17787-72-3 | CALCIUM ION | 40.078 | Ca | [Ca+2] | 1yag |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 1yag |
| 14808-79-8 | SULFATE ION | 96.063 | O4 S   | [O-]S([O-])(=O)=O | 1yag |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/440505/Q9U1E8.pdb 2.0 376 = residues | | = | | Ramachandran plot: 93.9% core 6.1% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 4 labelled residues (out of 374) = | | Chi1-chi2 plots: 0 labelled residues (out of 221) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 2.4 Bad contacts: = 0 | *| Bond len/angle: 9.2 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.14 Covalent: -0.12 Overall: = 0.04 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | *| M/c bond angles: 93.7% within limits 6.3% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |