| Nucleoside diphosphate kinase B [Q9U1E1] | |
|---|---|
| Systematic Name | LmjF.32.2950 [Leishmania major] |
| Gene Name | L1648.07 |
| Molecular Weight | 16640 Da |
| Protein Sequence Size | 151 |
| Function | |
| Charge | 2.5 |
| Isoelectric Point | 8.0622 pH |
| Description | Nucleoside diphosphate kinase B (EC 2.7.4.6). |
| Subcellular Location | nucleus[Predict] |
| E. C. Number | 2.7.4.6 |
| Sequence | >tr|Q9U1E1|Q9U1E1_LEIMA Nucleoside diphosphate kinase B (EC 2.7.4.6) - Leishmania major. SSERTFIAVKPDGVQRGLVGEIIARFERKGYKLVALKILQPTTEQAQGHYKDLCSKPFFP ALVKYFSSGPIVCMVWEGKNVVKSGRVLLGATNPADSQPGTIRGDFAVDVGRNVCHGSDS VESAEREIAFWFKADEIASWTSHSVSQIYE |
| DNA Sequence | >LmjF32.2950 |||nucleoside diphosphate kinase b|Leishmania major|chr 32|||Manual ATGTCCTCCG AGCGCACCTT TATTGCCGTC AAGCCGGACG GCGTGCAGCG CGGCCTCGTTGGCGAGATCA TCGCCCGCTT CGAGCGCAAG GGCTACAAGC TCGTCGCCTT GAAGATACTGCAGCCGACGA CGGAGCAGGC CCAGGGTCAC TATAAGGACC TTTGCTCCAA GCCGTTTTTCCCGGCCCTTG TGAAGTACTT CTCCTCTGGC CCGATCGTGT GTATGGTGTG GGAGGGTAAGAACGTGGTGA AGAGCGGCCG CGTGCTGCTC GGCGCGACGA ACCCGGCCGA CTCACAGCCCGGCACGATCC GTGGCGACTT TGCCGTGGAT GTGGGCCGCA ACGTGTGCCA CGGGTCCGACTCTGTGGAGA GCGCGGAGCG CGAGATCGCC TTTTGGTTCA AGGCGGATGA GATCGCGAGCTGGACGTCGC ACTCCGTGTC CCAGATCTAT GAGTAA |
| Nucleoside diphosphate kinase B Q9U1E1] | |
|---|---|
| Metabolite Information | CTP biosynthesis; GTP biosynthesis; UTP biosynthesis |
| Molecular Function | |
| Biochemical Pathway | |
| Regulatory Pathway | |
| KEGG Pathways | K00940 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | non-metastatic cells 2, protein (NM23B) expressed in [Homo sapiens] | 65 | 5e-55 | 209 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| ndk Nucleoside diphosphate kinase (EC 2.7.4.6) | Escherichia coli MG1655 | 50% | 2e-35 | 141 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00016 | Cell attachment sequence | 104-106; | PS00016 | |
| PDOC00409 | Nucleoside diphosphate kinases active site | 114-122; | PS00469 | |
| Acylation | N-myristoylation site | 14-19; 101-106; | PS00008 | |
| Phosphorylation | Protein kinase C phosphorylation site | 3-5; 85-87; 102-104; | PS00005 | |
| Nucleoside diphosphate kinase B [Q9U1E1] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1nueF | |
| Percent Identity | 65% | |
| Target Region | 1-151 | |
| Template Region | 2-151 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| LYS | 11 | Unknown |
| ASN | 114 | Unknown |
| Co-Factor | |
|---|---|
| Metal | Description |
| Mg | |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 146-91-8 | GUANOSINE-5'-DIPHOSPHATE | 443.201 | C10 H15 N5 O11 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 1nue |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 1nue |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2029820/Q9U1E1.pdb 2.0 151 = residues | | = | +| Ramachandran plot: 98.4% core 0.8% allow 0.8% gener 0.0% = disall | | = | +| All Ramachandrans: 1 labelled residues (out of 149) = | +| Chi1-chi2 plots: 1 labelled residues (out of 81) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | | Residue properties: Max.deviation: 2.8 Bad contacts: = 1 | +| Bond len/angle: 4.5 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.16 Covalent: -0.08 Overall: = 0.07 | | = | | M/c bond lengths: 99.6% within limits 0.4% highlighted = | | M/c bond angles: 94.9% within limits 5.1% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |