| Acetyl-CoA synthetase, putative [Q9U0W1] | |
|---|---|
| Systematic Name | LmjF.23.0710 [Leishmania major] |
| Gene Name | L7836.01 |
| Molecular Weight | 77409 Da |
| Protein Sequence Size | 695 |
| Function | |
| Charge | 0.5 |
| Isoelectric Point | 6.5468 pH |
| Description | Acetyl-CoA synthetase, putative (EC 6.2.1.1). |
| Subcellular Location | integral to membrane[Predict] |
| E. C. Number | 6.2.1.1 |
| Sequence | >tr|Q9U0W1|Q9U0W1_LEIMA Acetyl-CoA synthetase, putative (EC 6.2.1.1) - Leishmania major. SSQVVSSPVTLKSPLSVSQHVGEFNSVRDTNVVEPTEANRTKSHVGPHLGSRMRIYEYSI EHNDVFWAEIARRDFYWKTTWPDDQHVKSYNFDKSKGPIFVKWFEGAVTNICYNALDRHL PAHKDRVCFYFEGNDPSVTEKVTYGSMYTRVVELANVLKHQYGITKGDRVGLYLPMIPFA AVAMLACARIGAVASVIFGGFSAQAIVTRVTDCSPKLIITADASSRGDKPILLKTIADQA LDDCSVLGCNVPCLVFENMNRQFCKMKEGRDTWYGDALARLTPEQHEECPVEWMDAEDVL FLLYTSGSTGKPKAIVHTTAGYMVYASTTFMYSFDYHMDDVYFCTADIGWITGHSYVVYG PMIHCATSVLFEGVPNYPDYSRWWQLVEKYKVSILYTAPTAIRSLMQAGDDYVKVGNRST LRVLGSVGEPINVEAWKWLRDVGGEGHCDVSDTWWQTETGGHMITPMPGCTPMKPGSATL PFFGVQPVILDPMKLNEKQGPAEGLLAIRAPWPGMARTIYGDHARFEKTYFDVDGYYMTG DGARRDSDGYYWITGRVDDVLNVSGHRIGTSEIEDAVNTHPAVVESAVVGFPQSIKGEGI YVFLTFHQGTEVTPELLAAVKATVRKVIGPLATPDVLHPTQEGLPKTRSGKIVRRILRKI ATHNDDDLGDTSTLADPTVVEALKRSRAKWVDKK |
| DNA Sequence | >LmjF23.0710 |||acetyl-CoA synthetase, putative|Leishmania major|chr 23|||Manual ATGAGCAGCC AGGTCGTCTC CAGTCCGGTG ACTCTGAAGA GTCCGCTGTC AGTCTCCCAGCATGTAGGCG AGTTTAACAG TGTGCGGGAC ACGAACGTTG TGGAGCCGAC GGAGGCAAACCGGACAAAGT CGCACGTCGG CCCCCACCTT GGCTCCCGCA TGCGCATCTA CGAGTACTCCATCGAGCACA ATGACGTCTT CTGGGCCGAA ATTGCACGGC GCGACTTCTA CTGGAAGACGACCTGGCCGG ATGACCAGCA CGTCAAGTCG TACAACTTCG ACAAATCCAA GGGCCCCATCTTTGTGAAAT GGTTCGAGGG CGCCGTGACA AACATCTGCT ACAACGCGTT GGACCGCCACCTGCCGGCGC ACAAGGATCG CGTGTGCTTT TACTTTGAAG GCAACGACCC GAGTGTGACGGAAAAGGTCA CGTACGGCAG CATGTACACG AGGGTGGTGG AGCTGGCGAA CGTGCTGAAGCACCAGTACG GTATTACAAA GGGCGACCGC GTTGGCCTGT ATCTACCCAT GATCCCGTTCGCTGCAGTGG CCATGCTCGC CTGCGCCCGC ATCGGTGCGG TGGCCAGTGT CATCTTTGGCGGATTTTCGG CTCAGGCGAT CGTGACTCGT GTCACGGACT GCAGCCCGAA GCTCATCATCACTGCTGACG CGTCCTCTCG CGGTGATAAG CCGATTCTGC TCAAGACGAT CGCGGACCAGGCCCTGGACG ACTGCAGCGT CCTGGGGTGC AACGTACCGT GCCTCGTATT CGAAAACATGAACCGTCAAT TCTGCAAGAT GAAGGAAGGC CGCGATACCT GGTACGGCGA TGCCTTAGCGCGGCTGACGC CGGAGCAGCA CGAGGAGTGC CCGGTGGAGT GGATGGACGC GGAGGATGTGCTGTTCCTGC TCTACACCTC CGGCAGCACC GGGAAACCCA AGGCCATCGT GCACACGACGGCGGGCTACA TGGTGTACGC CTCCACCACC TTCATGTACA GCTTCGACTA CCACATGGACGACGTGTACT TCTGCACGGC TGATATTGGC TGGATCACAG GCCACAGCTA CGTCGTGTATGGTCCGATGA TCCACTGCGC CACGTCGGTG CTGTTCGAGG GTGTGCCGAA CTACCCCGACTACTCGCGCT GGTGGCAGCT CGTCGAGAAG TACAAGGTGT CCATTCTCTA CACCGCGCCCACCGCAATCC GCTCCCTCAT GCAGGCCGGC GACGACTACG TCAAGGTGGG CAACCGCAGCACCCTGCGTG TGCTGGGCTC CGTCGGCGAG CCGATCAACG TGGAGGCGTG GAAGTGGCTGCGCGATGTCG GCGGCGAGGG CCACTGCGAC GTGTCAGACA CGTGGTGGCA GACTGAGACGGGTGGCCACA TGATTACACC GATGCCGGGC TGCACCCCCA TGAAGCCCGG TAGTGCAACGCTGCCGTTCT TCGGTGTCCA GCCGGTTATT CTCGACCCCA TGAAGCTGAA TGAGAAGCAGGGCCCAGCGG AGGGCCTGCT CGCCATTCGT GCGCCGTGGC CAGGTATGGC CCGCACCATCTACGGCGACC ACGCACGCTT TGAGAAGACC TACTTTGACG TGGACGGGTA CTACATGACAGGCGACGGTG CTCGCCGCGA CTCGGACGGC TATTACTGGA TCACGGGCCG CGTCGATGACGTGCTGAACG TGAGCGGGCA CCGCATCGGC ACCAGCGAGA TCGAGGACGC CGTCAACACCCACCCGGCAG TGGTGGAGAG CGCCGTGGTC GGCTTCCCTC AAAGCATCAA GGGTGAGGGCATCTACGTCT TCCTTACGTT CCACCAGGGG ACGGAGGTGA CGCCCGAGCT GCTGGCGGCGGTAAAGGCGA CTGTGCGCAA GGTGATTGGT CCGCTGGCCA CGCCAGATGT GCTTCACCCCACGCAGGAAG GTCTGCCGAA AACGCGCTCC GGCAAGATTG TGCGCCGCAT CTTGCGCAAGATCGCCACTC ACAACGACGA CGACCTGGGC GACACCTCGA CCCTGGCAGA CCCCACCGTCGTGGAGGCGC TGAAGCGCTC GCGTGCAAAG TGGGTGGATA AAAAGTAG |
| Acetyl-CoA synthetase, putative Q9U0W1] | |
|---|---|
| Metabolite Information | |
| Molecular Function | AMP binding; acetate-CoA ligase activity; catalytic activity |
| Biochemical Pathway | metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K01895 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | acyl-CoA synthetase short-chain family member 2 isoform a [Homo sapiens] | 52 | 0 | 700 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| acsA Acetyl-CoA synthetase | Staphylococcus aureus | 35% | 7e-90 | 325 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00016 | Cell attachment sequence | 227-229; | PS00016 | |
| PDOC00427 | Putative AMP-binding domain signature | 303-314; | PS00455 | |
| Acylation | N-myristoylation site | 107-112; 134-139; 146-151; 164-169; 192-197; 200-205; 417-422; 515-520; 644-649; | PS00008 | |
| Glycosylation | N-glycosylation site | 40-43; 418-421; 563-566; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 545-548; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 27-30; 81-84; 138-141; 166-169; 226-229; 236-239; 427-430; 530-533; 572-575; 663-666; 674-677; 679-682; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 11-13; 27-29; 140-142; 215-217; 225-227; 310-312; 421-423; 555-557; 595-597; 624-626; 650-652; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 268-275; 529-537; 545-552; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 530-544; | PS00003 | |
| Acetyl-CoA synthetase, putative [Q9U0W1] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2p2fB | |
| Percent Identity | 54% | |
| Target Region | 1-695 | |
| Template Region | 5-632 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 71-50-1 | ACETATE ION | 59.044 | C2 H3 O2 | [O-]C(=O)C | 2p2f |
| 61-19-8 | ADENOSINE MONOPHOSPHATE  | 347.221 | C10 H14 N5 O7 P   | O=P(O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 2p2f |
| 31416-98-5 | COENZYME A | 767.534 | C21 H36 N7 O16 P3 S | O=C(NCCS)CCNC(=O)C(O)C(C)(C)COP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3OP(=O)(O)O | 2p2f |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/867030/Q9U0W1.pdb 2.0 695 = residues | | = | *| Ramachandran plot: 90.5% core 8.5% allow 0.8% gener 0.2% = disall | | = | +| All Ramachandrans: 19 labelled residues (out of 693) = | +| Chi1-chi2 plots: 6 labelled residues (out of 377) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 8.7 Bad contacts: = 17 | *| Bond len/angle: 8.0 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: -0.03 Covalent: -0.20 Overall: = -0.09 | | = | | M/c bond lengths: 98.8% within limits 1.2% highlighted = | | M/c bond angles: 92.9% within limits 7.1% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |