| ADP-ribosylation factor [Q9U0T6] | |
|---|---|
| Systematic Name | LmjF.04.0480 [Leishmania major] |
| Gene Name | P1421.05 |
| Molecular Weight | 21070 Da |
| Protein Sequence Size | 190 |
| Function | |
| Charge | -1 |
| Isoelectric Point | 6.2337 pH |
| Description | ADP-ribosylation factor. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q9U0T6|Q9U0T6_LEIMA ADP-ribosylation factor - Leishmania major. GNVFSSWFEGLFSNKNASIVMVGLDAAGKTTILDKLKLGASRETVPTIGFHVETVDYRNV TFHLWDVGGQKRLRALWKMYYEGANAVIFVVDSNDRARIGEVREELRTLLSDPLLAGAAL LVLCNKQDLPHRLTPAELVEGLGFRDLSDNGLGRYLAGHKWYVQGCCAHTGDGLYEGLDW MCSHLPDNV |
| DNA Sequence | >LmjF04.0480 |||ADP-ribosylation factor, putative|Leishmania major|chr 4|||Manual ATGGGCAACG TCTTTTCCAG CTGGTTCGAG GGCCTCTTCA GCAATAAAAA CGCTAGCATCGTCATGGTCG GCCTCGATGC AGCCGGCAAG ACAACCATTT TAGATAAACT GAAGCTCGGCGCCTCGCGCG AGACGGTGCC GACGATCGGC TTCCACGTGG AGACGGTGGA CTACCGCAACGTGACGTTTC ACCTGTGGGA CGTCGGTGGG CAGAAGCGGC TGCGCGCGCT GTGGAAGATGTACTACGAAG GTGCCAACGC CGTTATCTTC GTCGTCGACA GCAATGATCG CGCGCGCATCGGCGAGGTGC GTGAAGAGCT GCGGACGCTC TTGTCAGACC CGCTGCTGGC CGGCGCAGCATTACTGGTGC TGTGCAACAA GCAAGACCTG CCGCATCGGC TGACGCCTGC GGAGCTCGTAGAAGGGTTAG GCTTTCGTGA CCTCTCCGAC AACGGCCTGG GACGCTACCT GGCCGGCCACAAGTGGTACG TGCAAGGATG CTGTGCTCAT ACAGGTGACG GCTTATACGA AGGGCTGGACTGGATGTGCT CTCACCTCCC AGACAATGTT TAG |
| ADP-ribosylation factor Q9U0T6] | |
|---|---|
| Metabolite Information | |
| Molecular Function | GTP binding |
| Biochemical Pathway | intracellular protein transport; protein transport; small GTPase mediated signal transduction |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | ADP-ribosylation factor 1 [Homo sapiens] | 53 | 7e-54 | 206 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YPL218w GTP-binding protein of the ARF family | Saccharomyces cerevisiae | 31% | 2e-19 | 89.4 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 24-31; | PS00017 | |
| Acylation | N-myristoylation site | 2-7; 11-16; 24-29; 174-179; | PS00008 | |
| Glycosylation | N-glycosylation site | 17-20; 60-63; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 7-10; 32-35; 135-138; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 14-16; | PS00005 | |
| Prenylation | ADP-ribosylation factors family signature | 160-182; | PS01019 | |
| Sulfation | Tyrosine sulfation site | 169-183; | PS00003 | |
| ADP-ribosylation factor [Q9U0T6] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1rrgB | |
| Percent Identity | 53% | |
| Target Region | 1-190 | |
| Template Region | 2-177 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ALA | 26 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 146-91-8 | GUANOSINE-5'-DIPHOSPHATE | 443.201 | C10 H15 N5 O11 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 1rrg |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 1rrg |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2557204/Q9U0T6.pdb 2.0 190 = residues | | = | | Ramachandran plot: 93.9% core 6.1% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 3 labelled residues (out of 188) = | +| Chi1-chi2 plots: 1 labelled residues (out of 113) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 2.6 Bad contacts: = 1 | *| Bond len/angle: 5.6 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.02 Covalent: -0.15 Overall: = -0.04 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | | M/c bond angles: 92.7% within limits 7.3% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |