| Mercaptopyruvate sulfurtransferase [Q7K9G0] | |
|---|---|
| Systematic Name | LmjF.05.0970 [Leishmania major] |
| Gene Name | MST |
| Molecular Weight | 40155 Da |
| Protein Sequence Size | 370 |
| Function | |
| Charge | -2 |
| Isoelectric Point | 6.2935 pH |
| Description | Mercaptopyruvate sulfurtransferase (EC 2.8.1.2). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 2.8.1.2 |
| Sequence | >tr|Q7K9G0|Q7K9G0_LEIMA Mercaptopyruvate sulfurtransferase (EC 2.8.1.2) - Leishmania major. SAPAAAPKHPGKVFLDPSEVKDHLAEYRIVDCRYSLKIKDHGSIQYAKEHVKSAIRADVD TNLSKLVPTSTARHPLPPCAEFIDWCMANGMAGELPVLCYDDECGAMGGCRLWWMLNSLG ADAYVINGGFQACKAAGLEMESGEPSSLPRPATHWPFKTAFQHHYLVDEIPPNAIITDAR SADRFASTVRPYAADKMPGHIEGARNLPYTSHLVTRGDGKVLRSEEEIRHNIMTVVQGAG DAADLSSFVFSCGSGVTACINIALVHHLGLGHPYLYCGSWSEYSGLFRPPIMRSIIDDYG MCMQMQTPSLGDNPKANLDTMTLKVDGAPCERPDAEVQSAATHLHAGEAATVYFKSGRVV TIEVPAVPN |
| DNA Sequence | >LmjF05.0970 |MST||mercaptopyruvate sulfurtransferase|Leishmania major|chr 5|||Manual ATGTCTGCTC CTGCTGCTGC GCCGAAACAC CCGGGCAAGG TGTTCCTGGA CCCGAGCGAGGTAAAGGACC ACCTTGCTGA GTACCGCATC GTGGACTGCC GGTACAGCTT GAAGATAAAGGACCACGGCA GCATCCAGTA CGCGAAGGAG CACGTGAAGA GCGCCATCCG CGCCGATGTGGATACGAACC TCTCTAAGTT GGTGCCCACC AGCACCGCCC GGCATCCGCT ACCGCCCTGTGCTGAGTTTA TCGACTGGTG CATGGCGAAC GGGATGGCGG GAGAGCTGCC AGTGCTCTGCTACGATGACG AGTGCGGCGC CATGGGTGGA TGCCGCCTGT GGTGGATGCT GAACTCTCTTGGCGCCGACG CGTACGTGAT CAACGGCGGC TTTCAGGCCT GCAAGGCTGC GGGGCTGGAGATGGAGTCCG GCGAGCCCTC GTCGCTGCCA AGACCCGCAA CGCACTGGCC CTTCAAGACGGCCTTTCAGC ATCACTACCT TGTGGATGAA ATCCCGCCCA ACGCAATCAT CACCGACGCGCGCTCCGCCG ACCGCTTCGC CTCGACAGTA CGACCTTACG CCGCAGACAA GATGCCAGGCCACATCGAAG GTGCGCGTAA CCTCCCCTAC ACGTCGCACC TCGTGACACG CGGTGACGGCAAGGTGCTGC GCAGTGAGGA AGAGATCCGC CACAACATCA TGACCGTCGT GCAAGGCGCGGGTGACGCGG CTGATCTATC GAGCTTCGTC TTCTCCTGCG GCAGCGGCGT CACCGCCTGCATCAATATCG CCCTGGTGCA CCACCTCGGC CTGGGCCATC CGTACCTCTA CTGTGGCTCCTGGTCTGAGT ACAGCGGCCT CTTCCGCCCC CCCATAATGC GCAGCATCAT CGACGACTACGGCATGTGCA TGCAAATGCA GACCCCTAGC CTCGGCGACA ACCCGAAGGC AAACCTCGACACCATGACGC TGAAGGTCGA CGGCGCGCCC TGCGAGAGAC CCGATGCGGA GGTGCAGAGCGCCGCAACCC ACCTCCACGC TGGCGAGGCC GCTACCGTGT ACTTCAAGAG CGGCCGCGTCGTCACGATCG AGGTGCCGGC AGTGCCCAAC TAA |
| Mercaptopyruvate sulfurtransferase Q7K9G0] | |
|---|---|
| Metabolite Information | |
| Molecular Function | transferase activity |
| Biochemical Pathway | biological process unknown |
| Regulatory Pathway | |
| KEGG Pathways | K01010 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | 3-mercaptopyruvate sulfurtransferase [Homo sapiens] | 30 | 4e-16 | 83 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| Rv0236c PROBABLE CONSERVED TRANSMEMBRANE PROTEIN | Mycobacterium tuberculosis H37Rv | 30% | 0.17 | 31.2 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00016 | Cell attachment sequence | 217-219; | PS00016 | |
| PDOC00322 | Rhodanese signatures and profile | 15.785; 14.360 | 24-143; 171-293 | PS50206 |
| Acylation | N-myristoylation site | 106-111; 129-134; 239-244; 254-259; 256-261; | PS00008 | |
| Glycosylation | N-glycosylation site | 63-66; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 216-219; 225-228; 280-283; 295-298; 310-313; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 36-38; 72-74; 189-191; 323-325; 357-359; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 29-35; | PS00007 | |
| Mercaptopyruvate sulfurtransferase [Q7K9G0] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1okgA | |
| Percent Identity | 99% | |
| Target Region | 1-370 | |
| Template Region | 7-359 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 61 | Sidechain |
| HIS | 75 | Sidechain |
| SER | 255 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 17787-72-3 | CALCIUM ION | 40.078 | Ca | [Ca+2] | 1okg |
| SULFITE ION | 80.063 | O3 S | [O-]S([O-])=O | 1okg | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/641750/Q7K9G0.pdb 2.0 370 = residues | | = | +| Ramachandran plot: 90.7% core 8.0% allow 1.3% gener 0.0% = disall | | = | +| All Ramachandrans: 8 labelled residues (out of 368) = | | Chi1-chi2 plots: 0 labelled residues (out of 194) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 19.8 Bad contacts: = 3 | *| Bond len/angle: 17.1 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.02 Covalent: -0.31 Overall: = -0.11 | | = | *| M/c bond lengths: 98.4% within limits 1.6% highlighted 3 off = graph | *| M/c bond angles: 92.4% within limits 7.6% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |