| Aromatic amino acid hydroxylase-like [Q6WRI4] | |
|---|---|
| Systematic Name | LmjF.28.1280 [Leishmania major] |
| Gene Name | AAAHL |
| Molecular Weight | 51454 Da |
| Protein Sequence Size | 453 |
| Function | |
| Charge | -0.5 |
| Isoelectric Point | 6.4 pH |
| Description | Aromatic amino acid hydroxylase-like (Putative phenylalanine-4- hydroxylase, putative) (EC 1.14.16.1). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 1.14.16.1 |
| Sequence | >tr|Q6WRI4|Q6WRI4_LEIMA Aromatic amino acid hydroxylase-like (Putative phenylalanine-4- hydroxylase, putative) (EC 1.14.16.1) - Leishmania major. LLRSRLFLEAGMAAAAATLKAQYASPGSNIKGADKKTRCRTSLQVSLSTDKPGELCHLLS VFKPYSINISQVANRPRAYENKAPLRTIFLDVDAYIEDESMKKVMAELHAKFPNVVVTGS WVIPWYPTEPKDLDELDQSTLAAGEELQEDPENPHPGFHDEVYRARRREIVGLAKNYKTG DPIPIVNYTEEENRVWTVVYDHLTRLYPTHACQQYNYVFPLLLENGVLSRTKTPQLRDVS EFLNEATGFTVRPVTGLLTSRDFLNALAFRVFYSTQYIRHAAQPLYTPEPDMVHDIIGHL PLLSDPDFANFTQTIGLASLGASDELLDKLAKVYWYSVEFGLCSEGGRRKAYGAGILSSC GELEYALSDKPECVPWDPTMASKTPFPITKYQPLYFVAESFSDAQRKLEAWLSSQEKPLY TVYNSYSRRVQSYPKNTWNMLQEQMKRTNFTF |
| DNA Sequence | >LmjF28.1280 |||phenylalanine-4-hydroxylase, putative|Leishmania major|chr 28|||Manual ATGCTGCTGC GCAGTCGTTT GTTCCTAGAG GCTGGCATGG CTGCTGCCGC CGCCACCCTGAAGGCCCAGT ACGCCTCCCC TGGGAGCAAC ATCAAGGGCG CCGACAAGAA GACGCGGTGCCGCACGTCCC TGCAGGTGTC CCTCTCCACT GATAAGCCTG GCGAGCTGTG TCACCTGCTGAGCGTCTTCA AACCGTACAG CATCAACATC AGCCAGGTCG CCAACCGTCC TCGCGCGTACGAAAACAAGG CGCCGCTGCG CACCATCTTC CTCGACGTGG ACGCTTACAT TGAGGACGAAAGCATGAAGA AGGTCATGGC GGAGCTGCAC GCGAAATTTC CGAATGTAGT CGTAACCGGATCGTGGGTGA TTCCGTGGTA TCCGACGGAG CCCAAAGACC TCGACGAACT CGATCAGAGCACGCTCGCTG CCGGGGAGGA GCTACAAGAA GACCCGGAGA ACCCCCACCC CGGCTTCCACGACGAGGTCT ACCGCGCCCG CCGCCGCGAG ATTGTCGGGC TTGCCAAGAA CTACAAAACTGGAGATCCGA TCCCGATTGT AAACTACACA GAAGAGGAGA ACCGTGTGTG GACGGTGGTCTACGACCACC TGACCCGCCT GTACCCGACG CACGCGTGCC AGCAGTACAA CTACGTCTTCCCGCTGCTCC TCGAAAACGG TGTGCTGAGC CGCACCAAAA CGCCACAGCT GCGCGATGTGAGCGAGTTCC TGAACGAGGC GACGGGCTTC ACGGTGCGCC CGGTGACGGG GCTGTTGACGTCGCGCGACT TCTTGAACGC ACTGGCGTTC CGCGTCTTTT ACAGCACCCA GTACATTCGCCACGCGGCGC AGCCATTGTA CACGCCGGAG CCGGACATGG TTCACGACAT CATTGGTCACCTGCCCCTGC TCTCTGATCC CGACTTCGCC AACTTCACCC AGACGATCGG CCTCGCCTCCCTTGGCGCCA GCGATGAGCT GTTGGACAAG CTGGCAAAGG TCTACTGGTA TAGCGTCGAGTTCGGCCTCT GCAGCGAGGG CGGCCGCCGC AAGGCCTACG GTGCTGGTAT TTTATCAAGCTGTGGCGAGC TAGAGTACGC ACTGAGCGAC AAGCCGGAGT GCGTCCCGTG GGACCCCACAATGGCGTCCA AAACGCCGTT CCCCATCACC AAGTACCAAC CGCTGTACTT TGTGGCCGAAAGCTTCTCAG ATGCGCAGCG TAAGCTGGAG GCGTGGCTCA GCTCGCAGGA GAAGCCGCTGTACACCGTCT ACAACTCGTA CTCGCGGCGC GTGCAGTCGT ACCCGAAGAA TACGTGGAACATGCTCCAGG AGCAGATGAA GCGAACAAAC TTCACCTTCT AA |
| Aromatic amino acid hydroxylase-like Q6WRI4] | |
|---|---|
| Metabolite Information | |
| Molecular Function | iron ion binding; monooxygenase activity |
| Biochemical Pathway | aromatic amino acid family metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K00500 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | phenylalanine hydroxylase [Homo sapiens] | 43 | 8e-93 | 338 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| pheA chorismate mutase/prephenate dehydratase | Haemophilus influenzae | 26% | 0.37 | 30.4 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00316 | Biopterin-dependent aromatic amino acid hydroxylases signature | 291-302; | PS00367 | |
| Acylation | N-myristoylation site | 12-17; 173-178; 257-262; 356-361; | PS00008 | |
| Amidation | Amidation site | 347-350; | PS00009 | |
| Glycosylation | N-glycosylation site | 69-72; 188-191; 311-314; 450-453; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 190-193; 260-263; 305-308; 360-363; 401-404; 414-417; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 19-21; 50-52; 101-103; 251-253; 260-262; 369-371; 428-430; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 89-103; 182-196; 359-373; | PS00003 | |
| Aromatic amino acid hydroxylase-like [Q6WRI4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2phmA | |
| Percent Identity | 45% | |
| Target Region | 26-437 | |
| Template Region | 19-403 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| HIS | 270 | Sidechain |
| SER | 334 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| FE (III) ION | 55.845 | Fe | [Fe+3] | 2phm | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1190684/Q6WRI4.pdb 2.0 412 = residues | | = | +| Ramachandran plot: 91.6% core 7.5% allow 0.8% gener 0.0% = disall | | = | *| All Ramachandrans: 15 labelled residues (out of 410) = | +| Chi1-chi2 plots: 2 labelled residues (out of 242) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 3.5 Bad contacts: = 4 | *| Bond len/angle: 5.7 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.07 Covalent: -0.18 Overall: = -0.02 | | = | | M/c bond lengths: 98.6% within limits 1.4% highlighted = | | M/c bond angles: 92.7% within limits 7.3% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |