| Protein kinase, putative [Q4QJE7] | |
|---|---|
| Systematic Name | LmjF.05.0550 [Leishmania major] |
| Gene Name | LMJF_05_0550 |
| Molecular Weight | 36400 Da |
| Protein Sequence Size | 319 |
| Function | |
| Charge | 11 |
| Isoelectric Point | 9.5 pH |
| Description | Protein kinase, putative (Cdc2-related kinase, putative). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QJE7|Q4QJE7_LEIMA Protein kinase, putative (Cdc2-related kinase, putative) - Leishmania major. RSSGPTPARPTGFQRYQRQHKVGEGSYGKVFLCTDVVEGGTVAVKTSQWNSGEEGLSVSS IREVSLLKEIRHPNVVRLLDLFTEEKKLCIVFERMEKDLRSVLSTRQTPIVGRKLKHMMY QLLSALHACHSRRVVHRDIKPGNILVSADEQTVKLADFGMGRAFGLALQSYTYRIATLYY RAPEVLLGDRYYLPSVDMWSMGCVMAELALRRALFRGEGEYSQLITIFGIMGTPSERVWP GVSRLPHYNAEFPSWVPTSLEKYIPTLDPEGIALLKAMLRYDPQRRITALQAMQHPFFDD VRDECEARLQQQQQQQQS |
| DNA Sequence | >LmjF05.0550 |||protein kinase, putative|Leishmania major|chr 5|||Manual ATGCGGAGCA GCGGCCCCAC CCCAGCGCGG CCGACGGGCT TCCAGCGCTA CCAACGCCAGCACAAGGTGG GCGAGGGGTC CTATGGCAAG GTGTTCCTCT GCACCGATGT CGTCGAGGGCGGCACCGTCG CTGTGAAGAC GAGTCAGTGG AACTCCGGCG AGGAGGGCCT CTCCGTCTCCTCGATTCGCG AGGTATCGCT CTTGAAAGAG ATACGCCACC CGAACGTGGT GCGGCTGCTGGACCTCTTCA CCGAGGAGAA GAAGCTCTGC ATCGTGTTCG AGCGCATGGA GAAGGACCTGCGTAGCGTTC TGTCGACCCG GCAGACCCCT ATCGTGGGCA GGAAGCTGAA ACACATGATGTACCAGCTTC TGAGCGCCCT GCACGCTTGC CACAGCCGCC GCGTCGTGCA TCGCGACATCAAGCCCGGAA ACATTCTTGT GAGCGCCGAC GAGCAGACTG TGAAGCTCGC CGACTTTGGTATGGGGCGTG CCTTCGGCCT TGCCCTGCAG AGCTACACCT ACCGCATCGC CACGCTCTACTACCGCGCGC CAGAGGTTCT GCTCGGCGAT CGCTACTACC TACCTTCCGT GGATATGTGGTCTATGGGGT GCGTCATGGC CGAGCTGGCG CTGCGCCGCG CTCTGTTCCG CGGCGAGGGCGAGTACTCGC AGCTCATCAC CATCTTCGGT ATTATGGGGA CGCCGAGCGA ACGCGTGTGGCCTGGCGTGT CGCGGCTGCC GCACTACAAC GCTGAGTTTC CAAGCTGGGT GCCGACGTCGCTCGAGAAGT ACATACCTAC CCTCGATCCG GAGGGGATAG CGTTGCTCAA GGCGATGCTGCGGTACGACC CACAGCGCCG TATTACCGCA TTGCAGGCGA TGCAGCACCC CTTCTTCGACGATGTGCGAG ACGAGTGCGA AGCTCGGTTG CAACAGCAGC AGCAGCAGCA GCAGTCGTAA |
| Protein kinase, putative Q4QJE7] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity |
| Biochemical Pathway | cell cycle; protein amino acid phosphorylation |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | cyclin-dependent kinase 2 isoform 1 [Homo sapiens] | 47 | 2e-77 | 286 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YBR160w cyclin-dependent protein kinase | Saccharomyces cerevisiae | 43% | 2e-68 | 253 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00100 | Protein kinases signatures and profile | 23-46; | PS00107 | |
| PDOC00100 | Protein kinases signatures and profile | 135-147; | PS00108 | |
| PDOC00100 | Protein kinases signatures and profile | 43.41 | 17-299 | PS50011 |
| Acylation | N-myristoylation site | 40-45; 56-61; 160-165; 203-208; | PS00008 | |
| Amidation | Amidation site | 112-115; | PS00009 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 286-289; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 52-55; 61-64; 148-151; 234-237; 259-262; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 61-63; 105-107; 132-134; 153-155; 173-175; 236-238; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 22-28; | PS00007 | |
| Protein kinase, putative [Q4QJE7] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2iw8C | |
| Percent Identity | 46% | |
| Target Region | 13-320 | |
| Template Region | 1-265 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 127 | Sidechain |
| LYS | 129 | Sidechain |
| ASN | 132 | Sidechain |
| THR | 166 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| O6-CYCLOHEXYLMETHOXY-2-(4'-SULPHAMOYLANILINO) PURINE | 402.471 | C18 H22 N6 O3 S | O=S(=O)(N)c1ccc(cc1)Nc3nc2c(ncn2)c(n3)OCC4CCCCC4 | 2iw8 | |
| 96-27-5 | MONOTHIOGLYCEROL | 108.159 | C3 H8 O2 S | OCC(O)CS | 2iw8 |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1218780/Q4QJE7.pdb 2.0 307 = residues | | = | *| Ramachandran plot: 90.4% core 8.5% allow 0.4% gener 0.7% = disall | | = | *| All Ramachandrans: 10 labelled residues (out of 305) = | +| Chi1-chi2 plots: 2 labelled residues (out of 190) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 8.7 Bad contacts: = 6 | *| Bond len/angle: 7.9 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.06 Covalent: -0.25 Overall: = -0.05 | | = | | M/c bond lengths: 99.0% within limits 1.0% highlighted = | | M/c bond angles: 92.3% within limits 7.7% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |