| Methylthioadenosine phosphorylase, putative [Q4QJB9] | |
|---|---|
| Systematic Name | LmjF.05.0830 [Leishmania major] |
| Gene Name | LMJF_05_0830 |
| Molecular Weight | 33409 Da |
| Protein Sequence Size | 306 |
| Function | |
| Charge | 2 |
| Isoelectric Point | 6.7 pH |
| Description | Methylthioadenosine phosphorylase, putative. |
| Subcellular Location | cytoplasm[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QJB9|Q4QJB9_LEIMA Methylthioadenosine phosphorylase, putative - Leishmania major. YGNPHKEPVAIAVIGGSGVYKLNCLQDAVYHDVPTPYGNPSGQLCVAKVDGVPCVFLPRH GPHHQYNPSEINYRANICALKQMGVRYILAINAVGSLDESYKPGDLVLCDQIIDKTYMRK ATFFEDGVVVHADFAHPTSRIFNSIVHEALLRCFPDVAAGKGTFQIHSSGTLVTMEGPQF STKAESLLNKQMGGHLIGMTSATEARLAREAEIAYATVAMVTDMDAWSDAPHVDAAQVTK VMAANVEKAQRYPPEIIKSLAQNLFDDPAHHTLEYAIVTKPEHIPAETKQRIAPLVASKY PQFAP |
| DNA Sequence | >LmjF05.0830 |||methylthioadenosine phosphorylase, putative|Leishmania major|chr 5|||Manual ATGTACGGCA ACCCGCACAA GGAACCCGTC GCGATCGCCG TCATCGGCGG CTCTGGCGTCTACAAGCTGA ACTGCTTGCA GGATGCAGTG TACCACGACG TACCGACCCC GTACGGCAACCCGAGCGGGC AGCTGTGCGT GGCCAAGGTC GACGGTGTGC CGTGCGTCTT CCTGCCGCGTCACGGTCCAC ACCACCAGTA CAACCCCAGC GAGATCAACT ACCGCGCCAA CATCTGCGCGCTGAAGCAGA TGGGCGTGCG CTACATCCTC GCCATCAACG CTGTTGGATC GCTGGACGAGTCGTACAAGC CTGGCGACCT CGTGCTGTGC GACCAGATCA TCGACAAGAC GTACATGCGCAAGGCAACCT TCTTCGAGGA CGGCGTCGTC GTGCACGCGG ACTTCGCCCA CCCGACATCGCGCATCTTCA ACAGCATCGT GCATGAGGCG CTGCTGCGCT GCTTTCCAGA CGTGGCGGCCGGCAAGGGCA CGTTCCAGAT TCACAGCAGC GGCACCCTCG TGACGATGGA AGGCCCGCAGTTCAGCACTA AGGCCGAGTC CCTCCTCAAC AAGCAGATGG GCGGCCACCT CATCGGCATGACGTCGGCGA CGGAGGCGCG GCTGGCGCGT GAGGCGGAGA TCGCATACGC GACAGTGGCGATGGTGACCG ACATGGACGC GTGGAGCGAC GCGCCGCACG TGGACGCGGC GCAGGTGACGAAGGTGATGG CGGCGAATGT GGAGAAGGCG CAGCGCTACC CACCCGAGAT CATCAAGTCCCTCGCCCAGA ATCTCTTCGA TGATCCGGCG CACCACACCC TGGAGTACGC CATCGTGACAAAGCCGGAGC ACATCCCTGC CGAGACGAAG CAGCGCATCG CGCCGCTCGT GGCGAGCAAGTACCCGCAGT TCGCCCCGTA G |
| Methylthioadenosine phosphorylase, putative Q4QJB9] | |
|---|---|
| Metabolite Information | |
| Molecular Function | S-methyl-5-thioadenosine phosphorylase activity; transferase activity, transferring pentosyl groups |
| Biochemical Pathway | amino acid salvage |
| Regulatory Pathway | |
| KEGG Pathways | K00772 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | 5'-methylthioadenosine phosphorylase [Homo sapiens] | 38 | 1e-43 | 174 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| metK S-adenosylmethionine synthetase | Mycobacterium tuberculosis H37Rv | 26% | 1.9 | 27.3 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00954 | Purine and other phosphorylases family 2 signature | 58-98; | PS01240 | |
| Acylation | N-myristoylation site | 39-44; 171-176; 199-204; | PS00008 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 120-123; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 97-100; 123-126; 183-186; 202-205; 223-226; 280-283; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 101-103; 139-141; 182-184; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 210-216; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 95-109; | PS00003 | |
| Methylthioadenosine phosphorylase, putative [Q4QJB9] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1wtaA | |
| Percent Identity | 45% | |
| Target Region | 1-263 | |
| Template Region | 3-255 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 224 | Sidechain |
| ASP | 226 | Backbone amide |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 5426-35-7 | ADENINE | 135.127 | C5 H5 N5 | n1c(c2ncnc2nc1)N | 1wta |
| 14265-44-2 | PHOSPHATE ION | 94.971 | O4 P | [O-]P([O-])([O-])=O | 1wta |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1039335/Q4QJB9.pdb 2.0 263 = residues | | = | +| Ramachandran plot: 93.8% core 5.3% allow 0.9% gener 0.0% = disall | | = | +| All Ramachandrans: 6 labelled residues (out of 261) = | +| Chi1-chi2 plots: 1 labelled residues (out of 140) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 16.2 Bad contacts: = 1 | *| Bond len/angle: 6.8 Morris et al class: 1 = 1 2 | *| 4 cis-peptides = | | G-factors Dihedrals: 0.02 Covalent: -0.19 Overall: = -0.06 | | = | | M/c bond lengths: 98.8% within limits 1.2% highlighted = | | M/c bond angles: 93.1% within limits 6.9% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |