| NADH-ubiquinone oxidoreductase, mitochondrial, putative [Q4QJA4] | |
|---|---|
| Systematic Name | LmjF.05.0980 [Leishmania major] |
| Gene Name | LMJF_05_0980 |
| Molecular Weight | 54517 Da |
| Protein Sequence Size | 481 |
| Function | |
| Charge | 15.5 |
| Isoelectric Point | 8.4 pH |
| Description | NADH-ubiquinone oxidoreductase, mitochondrial, putative (EC 1.6.99.3). |
| Subcellular Location | Mitochondrion[Predict] |
| E. C. Number | 1.6.99.3 |
| Sequence | >tr|Q4QJA4|Q4QJA4_LEIMA NADH-ubiquinone oxidoreductase, mitochondrial, putative (EC 1.6.99.3) - Leishmania major. RQGWLRSSAALLERVHGHLKDQDRIFTNLYNDFGTGIDAAERRGDWYRTKDILLKGHDWV INEIKASGLRGRGGAGFPSGLKWSFMPKKKQDDRPSYIVVNCDESEPGTCKDREIMRHEP HKVVEGALLAGFAMRARYGYIYIRGEFYNEWRSVEKAIHEAYEKGYLGKNACGSGWDFDL YTYRGAGAYICGEETAMIASIEGGQGKPRLKPPFPANVGLYGCPTTVTNCETVAVSPTII RRGPQWFAQFGRKGNAGTKLFCISGHVNRPCTVEEEMSMPLRELLERHAGGVRGGWDNLL CVIPGGSSCPLIPKHICDDILMDYDALKEAQTGLGTAAVIVMDKSTDVINAIERLSQFYM HESCGQCTPCREGSPWLDKMMKRFVNGNAKKEEIYTLWDVSKQMEGRSICALGTAAAWPV QGLIRHFKPLMEERIERFWEANPHWGQPGSPWRRWKTHRYYTMQKGDRLNWDGKIVRNWN |
| DNA Sequence | >LmjF05.0980 |||NADH-ubiquinone oxidoreductase, mitochondrial, putative|Leishmania major|chr 5|||Manual ATGCGTCAGG GATGGCTCCG CTCATCCGCG GCGCTCTTGG AGCGCGTGCA CGGCCACCTCAAGGATCAGG ACCGCATTTT CACAAACCTG TACAATGACT TCGGCACCGG GATCGACGCGGCGGAGCGCC GTGGTGACTG GTACCGCACC AAGGACATCC TCCTCAAAGG CCATGACTGGGTGATCAACG AGATCAAGGC GAGCGGCCTT CGTGGCCGTG GCGGTGCCGG CTTCCCGTCCGGACTGAAGT GGTCCTTCAT GCCCAAGAAG AAGCAGGATG ACCGCCCAAG CTACATCGTGGTGAACTGCG ATGAGTCGGA GCCTGGCACG TGCAAGGACC GTGAGATCAT GCGCCACGAGCCGCACAAGG TGGTGGAGGG TGCACTGTTG GCCGGCTTTG CGATGCGGGC CCGCTACGGCTACATCTACA TCCGCGGTGA GTTCTACAAC GAGTGGCGCT CGGTCGAGAA GGCGATTCACGAGGCGTACG AAAAGGGCTA CCTCGGCAAG AACGCGTGCG GCAGCGGCTG GGACTTTGACCTCTACACCT ACCGCGGCGC CGGCGCGTAC ATCTGCGGTG AGGAGACGGC GATGATCGCCAGCATAGAGG GTGGCCAGGG CAAGCCTCGC CTGAAGCCGC CGTTCCCGGC GAACGTCGGCCTCTACGGGT GCCCGACGAC GGTGACGAAC TGTGAGACGG TGGCGGTGTC ACCGACCATAATCCGCCGTG GCCCGCAGTG GTTCGCGCAG TTCGGCCGCA AGGGCAACGC CGGCACGAAGCTCTTTTGCA TCTCCGGCCA CGTGAACCGC CCGTGCACCG TGGAGGAGGA GATGAGCATGCCTCTGCGCG AGCTGCTCGA ACGACACGCC GGCGGCGTGC GGGGCGGCTG GGACAACCTACTGTGCGTCA TCCCCGGCGG CTCCTCGTGC CCGCTTATCC CAAAGCACAT CTGTGACGACATCCTGATGG ACTACGATGC GCTCAAGGAG GCGCAGACTG GCCTTGGCAC GGCGGCGGTGATCGTCATGG ACAAGTCCAC CGACGTGATC AACGCGATTG AGCGCCTGTC GCAGTTCTACATGCACGAGT CGTGCGGCCA GTGCACGCCG TGCCGCGAGG GCAGCCCATG GCTGGACAAGATGATGAAGC GCTTCGTCAA CGGCAATGCC AAGAAGGAGG AAATCTATAC CCTGTGGGACGTGTCCAAGC AGATGGAGGG CCGGTCCATC TGCGCACTTG GGACGGCGGC GGCGTGGCCGGTGCAGGGAC TCATCCGCCA CTTCAAGCCC CTCATGGAGG AGCGCATCGA ACGCTTCTGGGAGGCGAACC CGCACTGGGG ACAACCTGGC TCTCCGTGGC GCCGCTGGAA GACGCACCGCTACTACACAA TGCAGAAGGG TGATCGTCTC AACTGGGACG GAAAGATTGT GCGCAACTGGAACTAG |
| NADH-ubiquinone oxidoreductase, mitochondrial, putative Q4QJA4] | |
|---|---|
| Metabolite Information | |
| Molecular Function | FMN binding; NAD binding; NADH dehydrogenase (ubiquinone) activity; iron ion binding; oxidoreductase activity, acting on NADH or NADPH |
| Biochemical Pathway | electron transport; mitochondrial electron transport, NADH to ubiquinone |
| Regulatory Pathway | |
| KEGG Pathways | K03942 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa [Homo sapiens] | 71 | 0 | 644 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| adh1 alcohol dehydrogenase I | Staphylococcus aureus | 31% | 0.4 | 30.4 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00016 | Cell attachment sequence | 44-46; | PS00016 | |
| PDOC00555 | Respiratory-chain NADH dehydrogenase 51 Kd subunit signatures | 363-374; | PS00645 | |
| Acylation | N-myristoylation site | 37-42; 81-86; 127-132; 188-193; 223-228; 255-260; 291-296; 292-297; 295-300; 334-339; 414-419; | PS00008 | |
| Amidation | Amidation site | 251-254; | PS00009 | |
| Phosphorylation | Casein kinase II phosphorylation site | 36-39; 110-113; 175-178; 229-232; 273-276; 397-400; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 110-112; 183-185; 458-460; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 90-98; | PS00007 | |
| NADH-ubiquinone oxidoreductase, mitochondrial, putative [Q4QJA4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2fugS | |
| Percent Identity | 47% | |
| Target Region | 7-447 | |
| Template Region | 7-429 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| FE2/S2 (INORGANIC) CLUSTER | 175.82 | Fe2 S2 | [Fe]1S[Fe]S1 | 2fug | |
| 6184-17-4 | FLAVIN MONONUCLEOTIDE | 456.344 | C17 H21 N4 O9 P | O=P(O)(O)OCC(O)C(O)C(O)CN2c3cc(c(cc3N=C1C2=NC(=O)NC1=O)C)C | 2fug |
| IRON/SULFUR CLUSTER | 351.64 | Fe4 S4 | [S]12[Fe]3[S]4[Fe]1[S]5[Fe]2[S]3[Fe]45 | 2fug | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1802178/Q4QJA4.pdb 2.0 440 = residues | | = | *| Ramachandran plot: 85.4% core 13.0% allow 0.8% gener 0.8% = disall | | = | *| All Ramachandrans: 22 labelled residues (out of 438) = | +| Chi1-chi2 plots: 4 labelled residues (out of 262) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 7.7 Bad contacts: = 13 | *| Bond len/angle: 6.4 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: -0.05 Covalent: -0.24 Overall: = -0.12 | | = | | M/c bond lengths: 98.7% within limits 1.3% highlighted = | *| M/c bond angles: 93.0% within limits 7.0% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |