| Electron transfer flavoprotein-ubiquinone oxidoreductase, putative [Q4QIN2] | |
|---|---|
| Systematic Name | LmjF.07.0600 [Leishmania major] |
| Gene Name | LMJF_07_0600 |
| Molecular Weight | 62796 Da |
| Protein Sequence Size | 575 |
| Function | |
| Charge | 8.5 |
| Isoelectric Point | 7.3801 pH |
| Description | Electron transfer flavoprotein-ubiquinone oxidoreductase, putative (EC 1.5.5.1). |
| Subcellular Location | Mitochondrion[Predict] |
| E. C. Number | 1.5.5.1 |
| Sequence | >tr|Q4QIN2|Q4QIN2_LEIMA Electron transfer flavoprotein-ubiquinone oxidoreductase, putative (EC 1.5.5.1) - Leishmania major. LRWTSRYLCASAAAAAGPEVHRDVEEFAVVIVGGGPSGLSAAIRLKQLAGDQRDSFRVGL VEKGSEIGAHTLSGACVEPHGLDELLPGWREQEGSVLSSMTPVTSDAFHVLIGPSRSVKV PWLPSTLHNRGNYITSLGGICKWLGEQAEALGVEIYPGFAAADVVVDEATGAVTGVQLND RGVDKSGRRTAQYEPGMIFRAKQTIFAEGCRGSCTKQLEKRFGLRTADNPQTFGLGIKEV WEVPKERHHPGTVMHTVGWPTTDKGHDNTYGGAFLYHYGDGLISCGYVVGLDYSNPYCRP YMEMQKWKTHDLIRGQLEGGRPILYGARTLVEGGFSALPKLHFPGGVLAGDCAGFLNLPK IKGTHTAMKSGMLAAEAVYEDAFKGGKEEAASGVECKSYDDCFRSSWLYKELYTVRNVRQ SFYKSFKWGMLYTGITSLLLHGREPWTLKHTTPDNLSLKPAKECVEIKYPKPDGTLTFDL LTNHSRSGTAHNADQPCHLRLKDSKVAEEVNLKAYAGPEARFCPAGVYEFVNDKLVINAQ NCLHCKACDIKDPTQNINWTVPEGGGGPNYQGQM |
| DNA Sequence | >LmjF07.0600 |||electron transfer flavoprotein-ubiquinone oxidoreductase, putative|Leishmania major|chr 7|||Manual ATGCTGCGCT GGACTTCGCG CTACTTGTGT GCCTCCGCTG CGGCTGCTGC GGGGCCTGAGGTGCACCGTG ACGTCGAGGA GTTCGCTGTT GTCATTGTCG GCGGTGGCCC TTCGGGACTCTCGGCTGCTA TTCGGCTGAA GCAGCTCGCC GGGGACCAGA GGGACTCTTT CCGCGTCGGCCTCGTTGAAA AGGGCAGCGA GATTGGCGCC CACACGCTCT CCGGCGCCTG CGTGGAACCTCACGGGTTGG ATGAGTTGCT GCCCGGATGG CGTGAGCAGG AGGGATCCGT TCTTTCCAGCATGACGCCCG TCACGTCCGA CGCCTTCCAT GTGCTGATAG GGCCGTCACG AAGCGTGAAGGTGCCGTGGC TGCCCTCGAC GCTGCACAAT CGCGGTAACT ACATCACGTC TCTCGGTGGCATATGCAAGT GGCTTGGCGA GCAGGCCGAG GCGCTCGGGG TGGAGATCTA CCCCGGCTTTGCGGCGGCAG ACGTCGTCGT TGACGAGGCG ACCGGCGCCG TGACAGGGGT GCAGCTGAATGACAGGGGCG TCGACAAGAG TGGCAGGAGG ACGGCGCAGT ACGAGCCCGG CATGATCTTCCGCGCGAAGC AGACCATCTT CGCCGAGGGC TGCCGCGGAT CCTGCACCAA GCAGTTGGAGAAGCGCTTTG GGCTGCGCAC CGCCGACAAC CCGCAGACGT TCGGGCTCGG TATCAAGGAGGTGTGGGAGG TGCCCAAAGA GCGGCACCAC CCAGGCACCG TCATGCACAC GGTGGGGTGGCCGACGACGG ACAAGGGGCA CGACAATACC TACGGCGGTG CCTTCCTCTA CCACTACGGCGACGGGCTCA TTTCGTGCGG CTACGTGGTG GGCCTCGACT ACTCGAACCC GTACTGCCGGCCGTACATGG AGATGCAGAA GTGGAAGACG CATGATCTCA TTCGAGGGCA GCTGGAGGGTGGCCGACCGA TCCTGTACGG TGCGCGCACG TTGGTCGAGG GCGGCTTCAG CGCGTTGCCGAAGCTGCACT TCCCCGGCGG CGTGCTGGCG GGGGACTGCG CTGGTTTCTT GAACCTTCCAAAGATCAAGG GCACGCACAC GGCCATGAAG TCTGGCATGC TGGCTGCCGA GGCCGTCTACGAGGACGCCT TCAAGGGCGG CAAGGAGGAG GCGGCCAGCG GCGTGGAATG CAAGAGCTACGACGACTGCT TCCGTAGCAG CTGGCTGTAC AAGGAGCTCT ACACGGTGCG CAACGTGCGGCAGAGCTTCT ACAAGAGCTT CAAGTGGGGC ATGCTGTACA CCGGCATCAC CTCACTCCTGCTGCACGGCC GCGAACCGTG GACGCTGAAG CACACCACAC CAGACAACTT GTCGCTGAAACCGGCGAAGG AATGCGTGGA GATTAAGTAC CCGAAGCCTG ACGGCACACT CACCTTCGACCTACTCACCA ACCACAGCCG TAGCGGGACG GCCCACAATG CTGACCAGCC CTGCCATCTGCGCTTGAAGG ACTCCAAGGT TGCGGAGGAG GTGAATCTGA AGGCGTATGC TGGGCCGGAGGCGCGCTTCT GCCCAGCTGG CGTGTACGAG TTCGTCAACG ACAAGCTTGT GATTAACGCGCAGAATTGCC TCCACTGCAA GGCGTGCGAC ATCAAGGACC CGACGCAGAA TATTAACTGGACGGTGCCCG AGGGCGGCGG TGGCCCCAAC TACCAGGGCC AGATGTAA |
| Electron transfer flavoprotein-ubiquinone oxidoreductase, putative Q4QIN2] | |
|---|---|
| Metabolite Information | |
| Molecular Function | electron-transferring-flavoprotein dehydrogenase activity; oxidoreductase activity |
| Biochemical Pathway | electron transport; metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K00311 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | electron-transferring-flavoprotein dehydrogenase [Homo sapiens] | 53 | 1e-170 | 595 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| STM0858 flavoprotein ubiquinone oxidoreductase | Salmonella typhimurium | 45% | 1e-139 | 488 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 456-459; 484-487; 559-562; | PS00722 | |
| Acylation | N-myristoylation site | 34-39; 35-40; 39-44; 65-70; 95-100; 132-137; 172-177; 176-181; 183-188; 210-215; 224-229; 282-287; 291-296; 316-321; 334-339; 346-351; 347-352; 364-369; 372-377; 430-435; 489-494; 566-571; | PS00008 | |
| Amidation | Amidation site | 187-190; | PS00009 | |
| Glycosylation | N-glycosylation site | 456-459; 484-487; 559-562; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 330-333; 393-396; 399-402; 452-455; 561-564; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 5-7; 56-58; 118-120; 187-189; 263-265; 415-417; 426-428; 448-450; 458-460; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 265-271; 463-470; | PS00007 | |
| Electron transfer flavoprotein-ubiquinone oxidoreductase, putative [Q4QIN2] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2gmjB | |
| Percent Identity | 53% | |
| Target Region | 1-575 | |
| Template Region | 7-578 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| THR | 72 | Unknown |
| CYS | 77 | Unknown |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 16426-55-4 | FLAVIN-ADENINE DINUCLEOTIDE | 785.55 | C27 H33 N9 O15 P2 | O=C2C3=Nc1cc(c(cc1N(C3=NC(=O)N2)CC(O)C(O)C(O)COP(=O)(O)OP(=O)(O)OCC6OC(n5cnc4c(ncnc45)N)C(O)C6O)C)C | 2gmj |
| IRON/SULFUR CLUSTER | 351.64 | Fe4 S4 | [S]12[Fe]3[S]4[Fe]1[S]5[Fe]2[S]3[Fe]45 | 2gmj | |
| 75-65-0 | TERTIARY-BUTYL ALCOHOL | 74.122 | C4 H10 O | OC(C)(C)C | 2gmj |
| Mutational Information | ||
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| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/951847/Q4QIN2.pdb 2.0 575 = residues | | = | *| Ramachandran plot: 90.5% core 8.2% allow 0.8% gener 0.4% = disall | | = | +| All Ramachandrans: 17 labelled residues (out of 573) = | +| Chi1-chi2 plots: 3 labelled residues (out of 311) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 10 | *| Bond len/angle: 8.4 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: -0.08 Covalent: -0.22 Overall: = -0.12 | | = | | M/c bond lengths: 99.3% within limits 0.7% highlighted = | *| M/c bond angles: 92.8% within limits 7.2% highlighted 3 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |