| Beta tubulin [Q4QI42] | |
|---|---|
| Systematic Name | LmjF.08.1230 [Leishmania major] |
| Gene Name | LMJF_08_1230 |
| Molecular Weight | 49707 Da |
| Protein Sequence Size | 443 |
| Function | |
| Charge | -20 |
| Isoelectric Point | 4.4708 pH |
| Description | Beta tubulin. |
| Subcellular Location | microtubule; protein complex[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QI42|Q4QI42_LEIMA Beta tubulin - Leishmania major. REIVSCQAGQCGNQIGSKFWEVIADEHGVDPTGTYQGDSDLQLERINVYFDESAGGRYVP RAVLMDLEPGTMDSVRAGPYGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELIDSVLDVCR KEAESCDCLQGFQLSHSLGGGTGSGMGTLLISKLREEYPDRIMMTFSVIPSPRVSDTVVE PYNTTLSVHQLVENSDESMCIDNEALYDICFRTLKLTTPTFGDLNHLVAAVMSGVTCCLR FPGQLNSDLRKLAVNLVPFPRLHFFMMGFAPLTSRGSQQYRGLSVAELTQQMFDAKNMMQ AADPRHGRYLTASALFRGRISTKEVDEQMLNVQNKNSSYFIEWIPNNIKSSICDIPPKGL KMSATFIGNNTCIQEMFRRVGEQFTGMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSE YQQYQDATVEEEGEYDEEQEAY |
| DNA Sequence | >LmjF08.1230 |||beta tubulin|Leishmania major|chr 8|||Manual ATGCGCGAGA TCGTTTCCTG CCAGGCCGGC CAGTGCGGCA ACCAGATCGG CTCCAAGTTTTGGGAGGTGA TTGCCGACGA ACATGGTGTC GATCCGACTG GTACCTACCA GGGCGACTCGGATCTGCAGC TGGAGCGCAT CAACGTCTAC TTCGATGAGT CGGCGGGAGG CCGCTACGTGCCGCGCGCCG TGCTGATGGA CCTCGAGCCC GGCACTATGG ACTCCGTTCG CGCCGGCCCGTACGGCCAGC TGTTCCGCCC GGACAACTTC ATCTTTGGTC AGTCCGGCGC TGGCAACAACTGGGCCAAGG GCCACTACAC CGAGGGCGCG GAGCTGATCG ACTCCGTGCT TGATGTGTGCCGCAAGGAGG CGGAGAGCTG CGACTGCCTG CAGGGCTTCC AGCTGTCTCA CTCCCTCGGCGGCGGCACGG GCTCCGGCAT GGGCACGCTG CTCATTTCCA AGCTGCGCGA GGAGTACCCGGACCGGATCA TGATGACCTT CTCCGTCATC CCGTCCCCCC GCGTGTCGGA TACCGTTGTGGAGCCGTACA ACACGACCCT CTCTGTGCAC CAGCTCGTGG AGAACTCCGA CGAGTCCATGTGCATCGACA ACGAGGCGCT GTACGATATT TGCTTCCGCA CGCTGAAGCT GACGACGCCGACGTTCGGTG ACCTGAACCA CCTCGTCGCC GCTGTGATGT CTGGCGTGAC CTGCTGCCTGCGCTTCCCTG GCCAGCTGAA CTCTGACCTG CGCAAGCTTG CCGTGAACCT CGTGCCGTTCCCGCGCCTGC ACTTCTTCAT GATGGGCTTC GCGCCGCTGA CGAGCCGCGG CTCGCAGCAGTACCGCGGCC TGTCCGTCGC GGAGCTGACG CAGCAGATGT TCGACGCCAA GAACATGATGCAGGCCGCCG ACCCGCGCCA CGGCCGCTAC CTCACCGCGT CCGCGCTGTT CCGCGGCCGCATATCGACCA AGGAGGTGGA CGAGCAGATG CTGAACGTGC AGAACAAGAA CTCCAGCTACTTCATCGAGT GGATCCCGAA CAACATCAAG TCGTCCATCT GCGATATCCC GCCCAAGGGACTCAAGATGT CCGCCACCTT CATCGGCAAC AACACCTGCA TCCAGGAGAT GTTCCGCCGCGTCGGTGAGC AGTTCACGGG TATGTTCCGC CGCAAGGCCT TCCTCCACTG GTACACCGGTGAGGGCATGG ACGAGATGGA GTTCACCGAG GCCGAGTCCA ACATGAACGA CCTCGTCTCCGAGTACCAGC AGTACCAGGA CGCCACCGTC GAGGAGGAGG GCGAGTACGA CGAGGAGCAGGAAGCCTACT AA |
| Beta tubulin Q4QI42] | |
|---|---|
| Metabolite Information | |
| Molecular Function | GTP binding; GTPase activity; structural constituent of cytoskeleton; structural molecule activity |
| Biochemical Pathway | cell motility; microtubule cytoskeleton organization and biogenesis; microtubule-based movement; protein polymerization |
| Regulatory Pathway | |
| KEGG Pathways | K07375 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | tubulin, beta, 2 [Homo sapiens] | 82 | 0 | 753 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YFL037w beta-tubulin | Saccharomyces cerevisiae | 68% | 0 | 634 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00199 | Tubulin subunits alpha, beta, and gamma signature | 140-146; | PS00227 | |
| PDOC00200 | Tubulin-beta mRNA autoregulation signal | 1-4; | PS00228 | |
| Acylation | N-myristoylation site | 10-15; 13-18; 29-34; 34-39; 71-76; 93-98; 96-101; 98-103; 140-145; 141-146; 142-147; 144-149; 235-240; 244-249; 283-288; 360-365; 369-374; | PS00008 | |
| Glycosylation | N-glycosylation site | 184-187; 337-340; 370-373; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 115-118; 178-181; 221-224; 285-288; 322-325; 352-355; 409-412; 429-432; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 75-77; 172-174; 214-216; 274-276; 322-324; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 43-57; 415-429; | PS00003 | |
| Beta tubulin [Q4QI42] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2hxhB | |
| Percent Identity | 81% | |
| Target Region | 1-427 | |
| Template Region | 2-426 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 58-64-0 | ADENOSINE-5'-DIPHOSPHATE | 427.201 | C10 H15 N5 O10 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 2hxh |
| 146-91-8 | GUANOSINE-5'-DIPHOSPHATE | 443.201 | C10 H15 N5 O11 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 2hxh |
| 86-01-1 | GUANOSINE-5'-TRIPHOSPHATE | 523.18 | C10 H16 N5 O14 P3 | O=P(O)(O)OP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 2hxh |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 2hxh |
| TAXOL | 853.906 | C47 H51 N O14 | O=C(c1ccccc1)NC(c2ccccc2)C(O)C(=O)OC5C(=C4C(OC(=O)C)C(=O)C7(C(C(OC(=O)c3ccccc3)C(O)(C4(C)C)C5)C6(OC(=O)C)C(OC6)CC7O)C)C | 2hxh | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2198365/Q4QI42.pdb 2.0 427 = residues | | = | *| Ramachandran plot: 87.6% core 10.3% allow 1.4% gener 0.8% = disall | | = | *| All Ramachandrans: 25 labelled residues (out of 425) = | +| Chi1-chi2 plots: 3 labelled residues (out of 257) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 12.2 Bad contacts: = 14 | *| Bond len/angle: 6.7 Morris et al class: 1 = 1 3 | | = | | G-factors Dihedrals: -0.13 Covalent: -0.21 Overall: = -0.15 | | = | | M/c bond lengths: 99.2% within limits 0.8% highlighted = | | M/c bond angles: 92.0% within limits 8.0% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |