| Isocitrate dehydrogenase [NADP], mitochondrial, putative [Q4QHI7] | |
|---|---|
| Systematic Name | LmjF.10.0290 [Leishmania major] |
| Gene Name | LMJF_10_0290 |
| Molecular Weight | 48490 Da |
| Protein Sequence Size | 435 |
| Function | |
| Charge | 8.5 |
| Isoelectric Point | 8.3273 pH |
| Description | Isocitrate dehydrogenase [NADP], mitochondrial, putative (EC 1.1.1.42). |
| Subcellular Location | Mitochondrion[Predict] |
| E. C. Number | 1.1.1.42 |
| Sequence | >tr|Q4QHI7|Q4QHI7_LEIMA Isocitrate dehydrogenase [NADP], mitochondrial, putative (EC 1.1.1.42) - Leishmania major. FRHVSAASASSLVAARSFSSTNVYLDKRIKVKNEVVDMDGDEMTRIIWSFIKEKLILPYV DVPINYFDLSVTNRDATNDKVTVEAAEAVKKCNVGIKCATITPDEARVKEFNLKKMWKSP NGTIRNILGGTVFREPIIVSNIPRIVPQWHNPIVVGRHAFGDQYKATDAVLKPGKLQLVH TPADGSAPTTLDVYDFKGEGVGLAMYNTKESIEGFAKSCFQYALMRKYPLVLTTKNTILK KYDGMFLQTFQRMYDEQYKADFEKAGITYNHRLIDDQVAQMIKGEGGFVWACKNYDGDVQ SDIVAQGFGSLGLMTSVLMCPDGKTIEAEAAHGTVTRHYRQHQQGKETSTNSVASIYAWT RGLAHRGKLDGNSDLVKFSETLERVVVKAIEDGHMTKDLALCVYGSSGVKREHYETTEQF LDSVDTALKKAMSA |
| DNA Sequence | >LmjF10.0290 |||isocitrate dehydrogenase [NADP], mitochondrial precursor, putative|Leishmania major|chr 10|||Manual ATGTTCCGCC ATGTTTCGGC TGCTTCGGCG AGCTCGCTCG TCGCCGCCCG CTCCTTCTCCAGCACCAACG TCTACCTGGA CAAGCGCATC AAGGTAAAGA ATGAGGTGGT AGACATGGACGGCGACGAGA TGACGCGCAT TATCTGGTCG TTCATCAAGG AAAAGCTGAT CCTGCCCTACGTGGATGTCC CGATCAACTA CTTCGACCTC AGCGTGACTA ACCGTGACGC GACGAACGACAAGGTCACGG TTGAAGCCGC CGAAGCGGTC AAGAAGTGCA ACGTTGGCAT CAAGTGCGCCACCATCACCC CCGATGAGGC CCGCGTGAAG GAGTTCAACC TGAAGAAGAT GTGGAAGAGTCCGAACGGCA CCATCCGGAA CATCCTTGGC GGCACGGTGT TCCGTGAGCC GATCATCGTCTCCAACATCC CACGTATCGT CCCTCAGTGG CACAATCCCA TCGTGGTCGG CCGCCACGCCTTCGGCGACC AGTACAAGGC GACGGACGCT GTTTTGAAGC CCGGTAAGCT GCAGCTCGTGCACACACCCG CTGACGGCAG CGCGCCGACA ACGCTGGATG TATACGACTT CAAGGGCGAGGGTGTCGGGT TGGCCATGTA CAACACGAAG GAGAGCATTG AGGGATTTGC GAAGAGCTGCTTCCAGTACG CGTTGATGCG CAAGTACCCA CTGGTTTTGA CGACGAAGAA CACCATCCTGAAGAAGTACG ATGGCATGTT CCTGCAGACC TTCCAGCGCA TGTATGACGA GCAGTACAAGGCCGACTTCG AGAAGGCCGG CATCACCTAC AATCACCGCC TCATCGATGA CCAGGTGGCGCAGATGATCA AGGGCGAGGG CGGCTTCGTG TGGGCGTGCA AGAACTACGA TGGCGACGTGCAGAGCGACA TTGTGGCGCA AGGCTTCGGC TCGCTGGGCC TCATGACATC TGTGCTGATGTGCCCGGATG GCAAGACAAT CGAGGCCGAG GCCGCCCACG GCACCGTGAC GCGTCACTACCGCCAGCATC AGCAGGGCAA GGAGACGAGC ACGAACTCCG TCGCCTCCAT CTACGCCTGGACGCGCGGTC TCGCCCATCG TGGCAAGCTT GACGGCAACA GCGACCTCGT CAAGTTCTCGGAGACGCTGG AGAGGGTGGT CGTCAAGGCG ATCGAGGATG GCCACATGAC GAAGGACCTGGCCCTCTGCG TCTACGGCTC CAGCGGCGTC AAGCGTGAAC ATTACGAGAC GACGGAGCAGTTCCTCGACA GCGTCGATAC AGCCCTGAAG AAGGCGATGA GCGCGTAA |
| Isocitrate dehydrogenase [NADP], mitochondrial, putative Q4QHI7] | |
|---|---|
| Metabolite Information | |
| Molecular Function | isocitrate dehydrogenase (NADP+) activity; oxidoreductase activity |
| Biochemical Pathway | isocitrate metabolism; main pathways of carbohydrate metabolism; metabolism; tricarboxylic acid cycle |
| Regulatory Pathway | |
| KEGG Pathways | K00031 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | isocitrate dehydrogenase 2 (NADP+), mitochondrial precursor [Homo sapiens] | 67 | 1e-165 | 578 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| leuB 3-isopropylmalate dehydrogenase (beta-IPM dehydrogenase) | Haemophilus influenzae | 26% | 0.0000001 | 51.6 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00389 | Isocitrate and isopropylmalate dehydrogenases signature | 295-314; | PS00470 | |
| Acylation | N-myristoylation site | 96-101; 123-128; 186-191; 201-206; 267-272; 313-318; | PS00008 | |
| Glycosylation | N-glycosylation site | 122-125; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 73-76; 103-106; 182-185; 190-193; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 73-75; 124-126; 234-236; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 253-259; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 188-202; 252-266; | PS00003 | |
| Isocitrate dehydrogenase [NADP], mitochondrial, putative [Q4QHI7] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1lwdB | |
| Percent Identity | 67% | |
| Target Region | 26-435 | |
| Template Region | 1-413 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| VAL | 155 | Sidechain |
| LYS | 211 | Sidechain |
| ASP | 251 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 6061-97-8 | ISOCITRIC ACID | 192.124 | C6 H8 O7 | O=C(O)C(CC(=O)O)C(O)C(=O)O | 1lwd |
| 16397-91-4 | MANGANESE (II) ION | 54.938 | Mn | [Mn+2] | 1lwd |
| 14808-79-8 | SULFATE ION | 96.063 | O4 S   | [O-]S([O-])(=O)=O | 1lwd |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2055351/Q4QHI7.pdb 2.0 410 = residues | | = | +| Ramachandran plot: 94.0% core 5.2% allow 0.8% gener 0.0% = disall | | = | +| All Ramachandrans: 5 labelled residues (out of 408) = | | Chi1-chi2 plots: 0 labelled residues (out of 246) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 9.5 Bad contacts: = 4 | *| Bond len/angle: 5.6 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.16 Covalent: -0.05 Overall: = 0.08 | | = | | M/c bond lengths: 99.7% within limits 0.3% highlighted = | | M/c bond angles: 95.6% within limits 4.4% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |