| Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal/mitochondrial [Q4QHG4] | |
|---|---|
| Systematic Name | LmjF.10.0510 [Leishmania major] |
| Gene Name | GPD |
| Molecular Weight | 39299 Da |
| Protein Sequence Size | 367 |
| Function | |
| Charge | 11.5 |
| Isoelectric Point | 9.3003 pH |
| Description | Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal/mitochondrial (EC 1.1.1.8). |
| Subcellular Location | cytoplasm; glycerol-3-phosphate dehydrogenase complex[Predict] |
| E. C. Number | 1.1.1.8 |
| Sequence | >tr|Q4QHG4|Q4QHG4_LEIMA Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal/mitochondrial (EC 1.1.1.8) - Leishmania major. ISTKRHINTNELLHLSKAVVFGSGAFGTALAMVLSKKCREVCVWHIKEEEARLVNEKREN DLYLRGVQLASNIIFTSDVDEAYKGAELILFVIPTQFLRGFFQKSGGNLIAYAKARQVPV LVCTKGIERSTLKFPAQIVGEFFPSNLLSVLAGPSFAIEVATGVFTCVSVASADINVARR LQRIMTTGDRSFVCWATTDTVGCEVASAVKNVLAIGSGVANGLGMGLNARAALITRGLLE IRDLTAALGGDGSAIFGLAGFGDLQLTCSSELSRNFTVGKKLGKGLSLEEIQRTSKAVAE GVATAEPLVRLAQQLKVTMPLCQQIYEVVYKKKNPRAAIADLLSCGLQDEGLPPLFKKSA ATPSKL |
| DNA Sequence | >LmjF10.0510 |GPD||glycerol-3-phosphate dehydrogenase [NAD+], glycosomal/mitochondrial|Leishmania major|chr 10|||Manual ATGATCTCCA CGAAGAGACA CATCAACACG AATGAACTGC TGCACCTGAG CAAGGCCGTCGTCTTCGGCT CCGGGGCCTT TGGCACGGCG CTGGCGATGG TGCTCTCCAA GAAGTGCCGCGAGGTATGCG TGTGGCACAT AAAGGAGGAG GAGGCGCGGC TCGTGAATGA GAAGCGCGAGAACGATCTCT ACCTCCGGGG AGTACAGCTT GCGTCGAACA TCATCTTTAC CTCGGATGTGGACGAGGCGT ACAAGGGTGC CGAGCTCATC CTCTTCGTCA TCCCGACCCA GTTCCTGCGCGGCTTCTTCC AGAAAAGTGG CGGCAACCTG ATCGCCTACG CGAAGGCGAG GCAGGTGCCGGTGCTTGTGT GCACGAAGGG TATTGAGCGC TCGACGCTGA AGTTCCCGGC GCAGATTGTTGGCGAGTTCT TCCCGAGCAA CCTGCTCTCT GTGCTCGCGG GCCCCAGCTT CGCCATCGAGGTGGCCACCG GCGTCTTCAC GTGCGTCAGC GTCGCCTCCG CCGACATCAA TGTGGCTCGCCGCCTGCAGC GCATCATGAC GACGGGCGAC CGCTCCTTTG TCTGCTGGGC CACCACCGACACCGTCGGCT GCGAGGTGGC AAGCGCCGTG AAGAACGTGC TGGCCATCGG CTCTGGCGTGGCGAATGGCC TCGGCATGGG TCTCAACGCG CGTGCCGCCC TCATCACGCG CGGCCTGCTCGAGATTCGAG ATCTGACGGC CGCGCTGGGC GGCGATGGCA GCGCCATCTT CGGCCTCGCCGGCTTCGGTG ACCTCCAGCT AACGTGCTCG TCGGAGCTGT CGCGTAACTT CACGGTGGGCAAAAAACTCG GCAAGGGTTT GTCTCTCGAG GAAATCCAAC GCACGAGCAA GGCCGTCGCGGAGGGCGTGG CGACGGCCGA GCCGCTGGTG CGCTTGGCAC AGCAGCTGAA GGTGACGATGCCGCTGTGCC AGCAAATCTA CGAGGTCGTG TACAAGAAGA AGAACCCACG CGCCGCCATCGCAGACCTCT TGTCGTGCGG CCTGCAGGAC GAGGGTCTGC CGCCGCTCTT CAAGAAGTCGGCTGCCACCC CCTCCAAGCT GTGA |
| Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal/mitochondrial Q4QHG4] | |
|---|---|
| Metabolite Information | |
| Molecular Function | NAD binding; glycerol-3-phosphate dehydrogenase (NAD+) activity; oxidoreductase activity, acting on CH-OH group of donors; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| Biochemical Pathway | carbohydrate metabolism; glycerol-3-phosphate catabolism; glycerol-3-phosphate metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K00057 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | glycerol-3-phosphate dehydrogenase 1 (soluble) [Homo sapiens] | 29 | 5e-25 | 112 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| gpsA NAD(P)H-dependent glycerol-3-phosphate | Bacillus subtilis | 34% | 8e-52 | 198 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 276-279; | PS00722 | |
| PDOC00299 | Microbodies C-terminal targeting signal | 365-367; | PS00342 | |
| Acylation | N-myristoylation site | 25-30; 28-33; 67-72; 108-113; 127-132; 164-169; 203-208; 217-222; 219-224; 223-228; 225-230; 250-255; 302-307; | PS00008 | |
| Amidation | Amidation site | 279-282; | PS00009 | |
| Glycosylation | N-glycosylation site | 276-279; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 78-81; 187-190; 288-291; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 3-5; 4-6; 36-38; 132-134; 295-297; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 77-91; | PS00003 | |
| Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal/mitochondrial [Q4QHG4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1n1gA | |
| Percent Identity | 88% | |
| Target Region | 1-367 | |
| Template Region | 9-346 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| LYS | 211 | Sidechain |
| THR | 268 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 2-BROMO-6-CHLORO-PURINE | 233.453 | C5 H2 Br Cl N4 | Clc1nc(Br)nc2ncnc12 | 1n1g | |
| 66321-94-6 | PALMITIC ACID | 256.424 | C16 H32 O2 | O=C(O)CCCCCCCCCCCCCCC | 1n1g |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/3264429/Q4QHG4.pdb 2.0 367 = residues | | = | *| Ramachandran plot: 94.1% core 5.6% allow 0.0% gener 0.3% = disall | | = | +| All Ramachandrans: 3 labelled residues (out of 365) = | +| Chi1-chi2 plots: 2 labelled residues (out of 196) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 6.3 Bad contacts: = 1 | *| Bond len/angle: 5.8 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.20 Covalent: -0.03 Overall: = 0.12 | | = | | M/c bond lengths: 99.7% within limits 0.3% highlighted = | | M/c bond angles: 96.4% within limits 3.6% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |