| Glucose-6-phosphate isomerase [Q4QGN9] | |
|---|---|
| Systematic Name | LmjF.12.0530 [Leishmania major] |
| Gene Name | PGI |
| Molecular Weight | 67169 Da |
| Protein Sequence Size | 605 |
| Function | |
| Charge | 2 |
| Isoelectric Point | 6.6815 pH |
| Description | Glucose-6-phosphate isomerase (EC 5.3.1.9) (GPI) (PGI) (PHI) (Phosphoglucose isomerase) (Phosphohexose isomerase) (EC 5.3.1.9). |
| Subcellular Location | glycosome[Predict] |
| E. C. Number | 5.3.1.9 |
| Sequence | >tr|Q4QGN9|Q4QGN9_LEIMA Glucose-6-phosphate isomerase (EC 5.3.1.9) (GPI) (PGI) (PHI) (Phosphoglucose isomerase) (Phosphohexose isomerase) (EC 5.3.1.9) - Leishmania major. SDYLSKLKEYVLESTEVNGCAPSMATSTFNAPYEVARRTKMLGATDSSLLSLPAWKRLQS LYEKHGNESILSHFESDHQRFGRYSIEVGLHSEDNFLFLDYSKSHINDEIKDALVALAEE RGVRAFAKAMFDGQRVNSTEDRAVLHVALRNRSNRPIIVDGKDVMTDVNNVLAQMKNFTE KVRSGEWKGQTGKSISNIVNIGIGGSDLGPVMVTEALKPFSKRDMHCFFVSNVDGTHMAE VLKQVNLEETIFIIASKTFTTQETLTNAMSARNALMDYLKAHNISTDGAVAKHFVALSTN TEKVREFGIDTVNMFVFWDWVGGRYSVWSAIGLSVMLSIGYDNFVEFLTGAHVMDNHFAS TPTEQNLPMMLALVGIWYNNFFGAETEAVLPYDQYLWRLPAYLQQLDMESNGKGVTKKSA AVAVQTGSIIFGEAGTNGQHAFYQLIHQGTKIIPCDFIGCVQTQNRVGDHHRILMSNFFA QTEALMVGKSAEEVRQELVKSGMSGEAMERMIPHKTFTGNRPSNSILVNALTPRALGAII AMYEHKVLVQGAIWGINSYDQWGVELGKVLAKSILPQLKSGNIVSDHDGSTNGLINMFNT RAHL |
| DNA Sequence | >LmjF12.0530 |PGI||glucose-6-phosphate isomerase|Leishmania major|chr 12|||Manual ATGAGCGATT ATCTTTCGAA GCTGAAGGAG TACGTGCTGG AGAGCACCGA AGTCAATGGGTGCGCCCCGA GCATGGCCAC TTCGACTTTC AATGCCCCGT ATGAGGTCGC ACGCAGGACCAAGATGCTGG GAGCCACAGA CAGCAGCCTG TTGAGCTTGC CTGCGTGGAA GCGTTTGCAGTCCCTGTACG AGAAGCACGG CAACGAGTCC ATCCTTTCGC ATTTCGAGAG CGATCATCAGCGCTTTGGGC GGTACTCGAT TGAGGTTGGC CTGCACAGCG AGGACAACTT CCTCTTCCTCGACTATTCCA AGTCGCATAT CAACGACGAG ATCAAGGACG CGTTGGTTGC GCTGGCCGAGGAACGTGGAG TGCGGGCATT TGCCAAGGCT ATGTTTGATG GGCAGCGGGT GAACTCTACTGAAGACCGCG CCGTGTTGCA TGTGGCGCTG CGTAACCGCA GTAACCGCCC AATTATCGTTGACGGGAAGG ATGTGATGAC CGATGTGAAC AATGTGCTTG CCCAAATGAA GAACTTTACCGAAAAGGTCC GCAGCGGAGA GTGGAAGGGT CAGACGGGCA AGAGCATTTC CAACATAGTCAACATCGGCA TTGGCGGCAG CGACCTTGGC CCGGTCATGG TGACCGAGGC ACTGAAGCCGTTCTCCAAGC GCGACATGCA CTGCTTTTTC GTGTCCAACG TCGATGGCAC ACACATGGCTGAGGTTCTGA AGCAGGTGAA CCTGGAGGAG ACCATCTTCA TCATTGCAAG CAAGACGTTCACCACGCAAG AAACGTTGAC GAATGCCATG TCTGCACGCA ACGCCCTCAT GGACTACCTAAAAGCACACA ACATCTCGAC GGATGGCGCC GTTGCAAAGC ATTTTGTTGC CCTATCGACAAACACGGAAA AGGTTCGTGA GTTTGGCATT GATACCGTCA ACATGTTTGT GTTCTGGGACTGGGTCGGTG GCCGCTACTC TGTGTGGTCC GCCATCGGTC TCTCCGTGAT GCTTTCGATTGGCTACGACA ACTTTGTGGA ATTCCTGACA GGCGCGCACG TGATGGATAA CCACTTTGCGTCTACACCGA CGGAGCAGAA CTTGCCGATG ATGCTGGCGT TGGTCGGCAT CTGGTACAACAACTTTTTCG GCGCGGAGAC AGAGGCGGTG CTGCCGTACG ACCAGTACCT TTGGCGTCTGCCGGCCTACC TTCAGCAGCT CGACATGGAG AGCAACGGCA AGGGCGTGAC CAAGAAGTCTGCTGCAGTGG CTGTGCAGAC GGGCTCCATT ATCTTCGGTG AGGCCGGCAC AAACGGTCAACACGCATTCT ACCAGCTCAT TCACCAGGGG ACCAAGATCA TCCCGTGCGA TTTCATTGGCTGCGTCCAAA CACAGAACCG TGTGGGTGAC CACCACCGGA TCCTGATGAG CAACTTTTTCGCGCAGACGG AGGCGCTCAT GGTAGGAAAA AGCGCGGAGG AGGTCCGTCA GGAGCTGGTCAAGTCTGGTA TGTCGGGTGA GGCTATGGAG AGGATGATTC CGCACAAAAC TTTTACGGGCAACCGCCCCA GCAACTCGAT CCTGGTGAAT GCTCTTACTC CGCGTGCGCT GGGTGCCATCATCGCCATGT ACGAGCACAA GGTTCTCGTC CAGGGCGCGA TCTGGGGCAT CAACAGCTATGACCAGTGGG GTGTGGAGCT TGGTAAGGTG CTTGCCAAGT CTATTTTGCC GCAGCTCAAGTCCGGCAACA TCGTCTCCGA TCACGACGGC TCTACGAATG GCCTGATCAA CATGTTCAACACGCGCGCAC ATCTGTGA |
| Glucose-6-phosphate isomerase Q4QGN9] | |
|---|---|
| Metabolite Information | |
| Molecular Function | glucose-6-phosphate isomerase activity |
| Biochemical Pathway | gluconeogenesis; glycolysis |
| Regulatory Pathway | |
| KEGG Pathways | K01810 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | glucose phosphate isomerase [Homo sapiens] | 58 | 0 | 633 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| pgi glucose-6-phosphate isomerase | Mycobacterium tuberculosis H37Rv | 46% | 1e-141 | 496 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 68-71; 138-141; 152-155; 178-181; 284-287; | PS00722 | |
| PDOC00299 | Microbodies C-terminal targeting signal | 603-605; | PS00342 | |
| PDOC00157 | Phosphoglucose isomerase signatures | 552-569; | PS00174 | |
| PDOC00157 | Phosphoglucose isomerase signatures | 320-333; | PS00765 | |
| Acylation | N-myristoylation site | 20-25; 44-49; 134-139; 203-208; 236-241; 323-328; 376-381; 460-465; 520-525; 538-543; 552-557; 564-569; 582-587; 590-595; | PS00008 | |
| Glycosylation | N-glycosylation site | 68-71; 138-141; 152-155; 178-181; 284-287; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 61-64; 73-76; 139-142; 222-225; 232-235; 261-264; 300-303; 362-365; 491-494; 586-589; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 154-156; 180-182; 192-194; 222-224; 271-273; 302-304; 417-419; 533-535; | PS00005 | |
| Glucose-6-phosphate isomerase [Q4QGN9] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1t10A | |
| Percent Identity | 91% | |
| Target Region | 49-605 | |
| Template Region | 49-556 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| LYS | 210 | Unknown |
| GLU | 216 | Unknown |
| GLY | 276 | Unknown |
| ARG | 277 | Unknown |
| GLU | 362 | Unknown |
| HIS | 393 | Unknown |
| LYS | 521 | Unknown |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 6814-87-5 | FRUCTOSE-6-PHOSPHATE | 260.136 | C6 H13 O9 P | O=P(O)(O)OCC1OC(O)(CO)C(O)C1O | 1t10 |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/602226/Q4QGN9.pdb 2.0 557 = residues | | = | *| Ramachandran plot: 93.4% core 5.8% allow 0.2% gener 0.6% = disall | | = | +| All Ramachandrans: 7 labelled residues (out of 555) = | *| Chi1-chi2 plots: 12 labelled residues (out of 338) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 5.2 Bad contacts: = 7 | *| Bond len/angle: 7.8 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.08 Covalent: -0.09 Overall: = 0.02 | | = | | M/c bond lengths: 99.7% within limits 0.3% highlighted = | | M/c bond angles: 94.6% within limits 5.4% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |