Alpha tubulin [Q4QGC5] | |
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Systematic Name | LmjF.13.0280 [Leishmania major] |
Gene Name | LMJF_13_0280 |
Molecular Weight | 49759 Da |
Protein Sequence Size | 451 |
Function | |
Charge | -18 |
Isoelectric Point | 4.6463 pH |
Description | Alpha tubulin. |
Subcellular Location | microtubule; protein complex[Predict] |
E. C. Number | N.A. |
Sequence | >tr|Q4QGC5|Q4QGC5_LEIMA Alpha tubulin - Leishmania major. REAICIHIGQAGCQVGNACWELFCLEHGIQPDGSMPSDKCIGVEDDAFNTFFSETGAGKH VPRCIFLDLEPTVVDEVRTGTYRQLFNPEQLVSGKEDAANNYARGHYTIGKEIVDLALDR IRKLADNCTGLQGFMVFHAVGGGTGSGLGALLLERLSVDYGKKSKLGYTVYPSPQVSTAV VEPYNCVLSTHSLLEHTDVATMLDNEAIYDLTRRSLDIERPSYTNVNRLIGQVVSSLTAS LRFDGALNVDLTEFQTNLVPYPRIHFVLTSYAPVVSAEKAYHEQLSVADITNSVFEPAGM LTKCDPRHGKYMSCCLMYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKCGINYQPPT VVPGGDLAKVQRAVCMIANSTAIAEVFARIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEA REDLAALEKDYEEVGAESADDMGEEDVEEY |
DNA Sequence | >LmjF13.0280 |||alpha tubulin|Leishmania major|chr 13|||Manual ATGCGTGAGG CTATCTGCAT CCACATCGGC CAGGCCGGCT GCCAGGTCGG TAACGCGTGCTGGGAGCTGT TCTGCCTTGA GCACGGCATC CAGCCTGATG GCTCCATGCC CTCTGACAAGTGCATCGGTG TTGAGGATGA CGCGTTCAAC ACGTTCTTCT CGGAGACCGG TGCTGGCAAGCACGTTCCGC GCTGCATCTT CCTGGACCTC GAGCCTACGG TCGTGGATGA GGTGCGCACCGGCACGTACC GCCAGCTGTT CAACCCCGAG CAGCTGGTGT CTGGCAAGGA GGATGCGGCGAACAACTACG CTCGTGGCCA CTACACGATC GGCAAGGAGA TCGTCGACCT TGCGCTGGACCGCATTCGCA AGCTGGCGGA CAACTGCACT GGTCTCCAGG GCTTTATGGT GTTCCACGCTGTGGGTGGCG GCACCGGCTC TGGCCTCGGT GCGCTGCTGC TGGAGCGCCT GTCTGTGGACTACGGCAAGA AGTCCAAGCT CGGCTACACC GTGTACCCGA GCCCGCAGGT GTCGACTGCCGTCGTGGAGC CGTACAACTG CGTGCTGTCG ACGCACTCGC TGCTCGAGCA CACCGATGTTGCGACGATGC TCGACAATGA GGCCATCTAC GACCTCACTC GTCGTTCTCT CGACATTGAGCGCCCGTCGT ACACGAACGT GAACCGCCTG ATCGGCCAGG TGGTGTCGTC TCTGACGGCGTCGCTGCGCT TCGATGGTGC GCTGAACGTG GACCTGACGG AGTTCCAGAC GAACCTTGTGCCGTACCCGC GCATCCACTT CGTGCTGACG AGCTACGCTC CGGTGGTGTC TGCCGAGAAGGCGTACCACG AGCAGCTGTC CGTCGCGGAC ATCACGAACT CGGTGTTTGA GCCTGCTGGCATGCTGACGA AGTGCGATCC TCGCCACGGC AAGTACATGT CGTGCTGCCT CATGTACCGCGGTGATGTCG TGCCGAAGGA TGTCAACGCC GCGATTGCGA CGATCAAGAC GAAGCGCACAATTCAGTTCG TGGACTGGTG CCCGACCGGC TTCAAGTGCG GCATCAACTA CCAGCCGCCGACCGTTGTGC CCGGCGGTGA CCTCGCGAAG GTGCAGCGCG CCGTGTGCAT GATTGCCAACTCGACCGCGA TCGCTGAGGT GTTTGCCCGC ATCGACCACA AGTTCGACCT GATGTACAGCAAGCGCGCGT TTGTGCACTG GTACGTGGGT GAGGGCATGG AGGAGGGCGA GTTCTCCGAGGCGCGCGAGG ATCTCGCTGC GCTGGAGAAG GACTACGAGG AGGTTGGCGC CGAGTCCGCCGACGACATGG GCGAGGAGGA CGTCGAGGAG TACTAA |
Alpha tubulin Q4QGC5] | |
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Metabolite Information | |
Molecular Function | GTP binding; GTPase activity; structural constituent of cytoskeleton; structural molecule activity |
Biochemical Pathway | cell motility; microtubule cytoskeleton organization and biogenesis; microtubule-based movement; protein polymerization |
Regulatory Pathway | |
KEGG Pathways | K07374 |
Orthologs | ||||
Homologs | GI | Percent Identity | Evalue | Score |
Homo sapiens | tubulin, alpha 3 [Homo sapiens] | 80 | 0 | 773 |
DEG Information | ||||
DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
YML085c alpha-1 tubulin | Saccharomyces cerevisiae | 66% | 1e-176 | 612 |
Post Translational Modification | ||||
PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
PDOC00016 | Cell attachment sequence | 320-322; | PS00016 | |
PDOC00199 | Tubulin subunits alpha, beta, and gamma signature | 142-148; | PS00227 | |
Acylation | N-myristoylation site | 10-15; 13-18; 34-39; 142-147; 143-148; 144-149; 146-151; 232-237; 365-370; 412-417; 436-441; | PS00008 | |
Amidation | Amidation site | 161-164; | PS00009 | |
Glycosylation | N-glycosylation site | 128-131; 380-383; | PS00001 | |
Phosphorylation | Casein kinase II phosphorylation site | 73-76; 94-97; 193-196; 202-205; 287-290; 294-297; 303-306; 439-442; | PS00006 | |
Phosphorylation | Protein kinase C phosphorylation site | 38-40; 82-84; 94-96; 213-215; 241-243; 334-336; 337-339; 400-402; | PS00005 | |
Sulfation | Tyrosine sulfation site | 425-439; | PS00003 |
Alpha tubulin [Q4QGC5] | ||
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Model Information | ||
Template PDB ID | 1sa1C | |
Percent Identity | 80% | |
Target Region | 1-451 | |
Template Region | 2-430 |
Domain Information | ||
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Domains | Start | End |
Active Site Information | ||
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Residue | Active Site Number | Functional Part |
Co-Factor | |
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Metal | Description |
Ligands | |||||
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CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
146-91-8 | GUANOSINE-5'-DIPHOSPHATE | 443.201 | C10 H15 N5 O11 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 1sa1 |
86-01-1 | GUANOSINE-5'-TRIPHOSPHATE | 523.18 | C10 H16 N5 O14 P3 | O=P(O)(O)OP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 1sa1 |
7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 1sa1 |
518-28-5 | 9-HYDROXY-5-(3,4,5-TRIMETHOXYPHENYL)-5,8,8A,9- TETRAHYDROFURO[3',4':6,7]NAPHTHO[2,3-D][1,3]DIOXOL- 6(5AH)-ONE | 414.405 | C22 H22 O8 | O=C4OCC5C(O)c2cc1OCOc1cc2C(c3cc(OC)c(OC)c(OC)c3)C45 | 1sa1 |
Mutational Information | ||
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Residue | Feature | Description |
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Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/783112/Q4QGC5.pdb 2.0 451 = residues | | = | *| Ramachandran plot: 86.3% core 9.9% allow 2.8% gener 1.0% = disall | | = | *| All Ramachandrans: 35 labelled residues (out of 449) = | +| Chi1-chi2 plots: 7 labelled residues (out of 253) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.9 Bad contacts: = 17 | *| Bond len/angle: 7.3 Morris et al class: 1 = 1 3 | | = | | G-factors Dihedrals: -0.24 Covalent: -0.36 Overall: = -0.27 | | = | | M/c bond lengths: 99.1% within limits 0.9% highlighted = | | M/c bond angles: 89.1% within limits 10.9% highlighted = | +| Planar groups: 99.4% within limits 0.6% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |