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Alpha tubulin [Q4QGC5]
Systematic NameLmjF.13.0280 [Leishmania major]
Gene NameLMJF_13_0280
Molecular Weight49759 Da
Protein Sequence Size451
Function
Charge-18
Isoelectric Point4.6463 pH
DescriptionAlpha tubulin.
Subcellular Locationmicrotubule; protein complex[Predict]
E. C. Number N.A.
Sequence>tr|Q4QGC5|Q4QGC5_LEIMA Alpha tubulin - Leishmania major.
REAICIHIGQAGCQVGNACWELFCLEHGIQPDGSMPSDKCIGVEDDAFNTFFSETGAGKH
VPRCIFLDLEPTVVDEVRTGTYRQLFNPEQLVSGKEDAANNYARGHYTIGKEIVDLALDR
IRKLADNCTGLQGFMVFHAVGGGTGSGLGALLLERLSVDYGKKSKLGYTVYPSPQVSTAV
VEPYNCVLSTHSLLEHTDVATMLDNEAIYDLTRRSLDIERPSYTNVNRLIGQVVSSLTAS
LRFDGALNVDLTEFQTNLVPYPRIHFVLTSYAPVVSAEKAYHEQLSVADITNSVFEPAGM
LTKCDPRHGKYMSCCLMYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKCGINYQPPT
VVPGGDLAKVQRAVCMIANSTAIAEVFARIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEA
REDLAALEKDYEEVGAESADDMGEEDVEEY
DNA Sequence>LmjF13.0280 |||alpha tubulin|Leishmania major|chr 13|||Manual
ATGCGTGAGG CTATCTGCAT CCACATCGGC CAGGCCGGCT GCCAGGTCGG TAACGCGTGCTGGGAGCTGT TCTGCCTTGA GCACGGCATC CAGCCTGATG GCTCCATGCC CTCTGACAAGTGCATCGGTG TTGAGGATGA CGCGTTCAAC ACGTTCTTCT CGGAGACCGG TGCTGGCAAGCACGTTCCGC GCTGCATCTT CCTGGACCTC GAGCCTACGG TCGTGGATGA GGTGCGCACCGGCACGTACC GCCAGCTGTT CAACCCCGAG CAGCTGGTGT CTGGCAAGGA GGATGCGGCGAACAACTACG CTCGTGGCCA CTACACGATC GGCAAGGAGA TCGTCGACCT TGCGCTGGACCGCATTCGCA AGCTGGCGGA CAACTGCACT GGTCTCCAGG GCTTTATGGT GTTCCACGCTGTGGGTGGCG GCACCGGCTC TGGCCTCGGT GCGCTGCTGC TGGAGCGCCT GTCTGTGGACTACGGCAAGA AGTCCAAGCT CGGCTACACC GTGTACCCGA GCCCGCAGGT GTCGACTGCCGTCGTGGAGC CGTACAACTG CGTGCTGTCG ACGCACTCGC TGCTCGAGCA CACCGATGTTGCGACGATGC TCGACAATGA GGCCATCTAC GACCTCACTC GTCGTTCTCT CGACATTGAGCGCCCGTCGT ACACGAACGT GAACCGCCTG ATCGGCCAGG TGGTGTCGTC TCTGACGGCGTCGCTGCGCT TCGATGGTGC GCTGAACGTG GACCTGACGG AGTTCCAGAC GAACCTTGTGCCGTACCCGC GCATCCACTT CGTGCTGACG AGCTACGCTC CGGTGGTGTC TGCCGAGAAGGCGTACCACG AGCAGCTGTC CGTCGCGGAC ATCACGAACT CGGTGTTTGA GCCTGCTGGCATGCTGACGA AGTGCGATCC TCGCCACGGC AAGTACATGT CGTGCTGCCT CATGTACCGCGGTGATGTCG TGCCGAAGGA TGTCAACGCC GCGATTGCGA CGATCAAGAC GAAGCGCACAATTCAGTTCG TGGACTGGTG CCCGACCGGC TTCAAGTGCG GCATCAACTA CCAGCCGCCGACCGTTGTGC CCGGCGGTGA CCTCGCGAAG GTGCAGCGCG CCGTGTGCAT GATTGCCAACTCGACCGCGA TCGCTGAGGT GTTTGCCCGC ATCGACCACA AGTTCGACCT GATGTACAGCAAGCGCGCGT TTGTGCACTG GTACGTGGGT GAGGGCATGG AGGAGGGCGA GTTCTCCGAGGCGCGCGAGG ATCTCGCTGC GCTGGAGAAG GACTACGAGG AGGTTGGCGC CGAGTCCGCCGACGACATGG GCGAGGAGGA CGTCGAGGAG TACTAA
Alpha tubulin Q4QGC5]
Metabolite Information
Molecular FunctionGTP binding; GTPase activity; structural constituent of cytoskeleton; structural molecule activity
Biochemical Pathwaycell motility; microtubule cytoskeleton organization and biogenesis; microtubule-based movement; protein polymerization
Regulatory Pathway
KEGG PathwaysK07374
Orthologs
Homologs GI Percent Identity Evalue Score
Homo sapienstubulin, alpha 3 [Homo sapiens]800773
DEG Information
DEG Protein DEG Organism Percent Identity Evalue Bit Score
YML085c alpha-1 tubulinSaccharomyces cerevisiae66%1e-176612
Post Translational Modification
PTM Type PTM Sub Type Score Modification Site Prosite ID
PDOC00016Cell attachment sequence320-322; PS00016
PDOC00199Tubulin subunits alpha, beta, and gamma signature142-148; PS00227
AcylationN-myristoylation site10-15; 13-18; 34-39; 142-147; 143-148; 144-149; 146-151; 232-237; 365-370; 412-417; 436-441; PS00008
AmidationAmidation site161-164; PS00009
GlycosylationN-glycosylation site128-131; 380-383; PS00001
PhosphorylationCasein kinase II phosphorylation site73-76; 94-97; 193-196; 202-205; 287-290; 294-297; 303-306; 439-442; PS00006
PhosphorylationProtein kinase C phosphorylation site38-40; 82-84; 94-96; 213-215; 241-243; 334-336; 337-339; 400-402; PS00005
SulfationTyrosine sulfation site425-439; PS00003
Alpha tubulin [Q4QGC5]
Model Information
Template PDB ID1sa1C
Percent Identity80%
Target Region1-451
Template Region2-430
Domain Information
Domains Start End
Active Site Information
Residue Active Site Number Functional Part
Co-Factor
Metal Description
Ligands
CAS number Name Mol. Weight Mol. Formula Smile Notation PDB Reference
146-91-8GUANOSINE-5'-DIPHOSPHATE443.201C10 H15 N5 O11 P2O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O1sa1
86-01-1GUANOSINE-5'-TRIPHOSPHATE 523.18C10 H16 N5 O14 P3O=P(O)(O)OP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O1sa1
7791-18-6MAGNESIUM ION24.305Mg[Mg+2]1sa1
518-28-59-HYDROXY-5-(3,4,5-TRIMETHOXYPHENYL)-5,8,8A,9- TETRAHYDROFURO[3',4':6,7]NAPHTHO[2,3-D][1,3]DIOXOL- 6(5AH)-ONE414.405C22 H22 O8O=C4OCC5C(O)c2cc1OCOc1cc2C(c3cc(OC)c(OC)c(OC)c3)C451sa1
Mutational Information
Residue Feature Description
Modeled Protein Template Structure
+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/783112/Q4QGC5.pdb 2.0 451 = residues | | = | *| Ramachandran plot: 86.3% core 9.9% allow 2.8% gener 1.0% = disall | | = | *| All Ramachandrans: 35 labelled residues (out of 449) = | +| Chi1-chi2 plots: 7 labelled residues (out of 253) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.9 Bad contacts: = 17 | *| Bond len/angle: 7.3 Morris et al class: 1 = 1 3 | | = | | G-factors Dihedrals: -0.24 Covalent: -0.36 Overall: = -0.27 | | = | | M/c bond lengths: 99.1% within limits 0.9% highlighted = | | M/c bond angles: 89.1% within limits 10.9% highlighted = | +| Planar groups: 99.4% within limits 0.6% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further.
Overlapped Structure Procheck Summary
LeishBase: Leishmania Structural Database