| Cystathionine beta-lyase-like protein [Q4QFT8] | |
|---|---|
| Systematic Name | LmjF.14.0460 [Leishmania major] |
| Gene Name | LMJF_14_0460 |
| Molecular Weight | 50098 Da |
| Protein Sequence Size | 459 |
| Function | |
| Charge | 1 |
| Isoelectric Point | 6.5 pH |
| Description | Cystathionine beta-lyase-like protein (EC 4.4.1.8). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 4.4.1.8 |
| Sequence | >tr|Q4QFT8|Q4QFT8_LEIMA Cystathionine beta-lyase-like protein (EC 4.4.1.8) - Leishmania major. MHAISAAKTTINRTILTALEEERQYLEQAMLPVSYKFPKGADYSELPTYPKRPHFDQAAK HPSTQVVIFDGCPNDPYHPTSMPIYQTATFVQPDIEEFGPYDYSRSGNPTRTAIETLVAN LEGAHAAFAFSSGMAALQTLVTTLNAGDTILASSDLYGGMHRLLTQITSCLGMTVVFVPM WDTEAVRQALVDNPAAKLLHMESPTNPLMRIVDIRAVCEAAHAHNVKVSIDNTMMSPLRC TPLALGCDYSIHSATKFLCGHSDTMCGLICVKSEEDVKRVAFLQNAQGNALAPFDCWLLL RGIRTLSIRVEKQELNAVAVALFLSRQSHVVSRLHYAGLNPATSPQISSLTEENFYLHRS QTSGPGSVLSIETGSVKRSNAFVRACKLFKLTVSFGSCNSLVEMPCLLSHASIPKEKRTL PVDLIRLSVGIEQIEDILADLTQAIAAAVAIHDNDGSC |
| DNA Sequence | >LmjF14.0460 |||cystathionine beta-lyase-like protein|Leishmania major|chr 14|||Manual ATGATGCACG CGATCAGCGC CGCCAAAACC ACCATCAATC GCACTATTCT GACGGCGCTGGAGGAGGAGC GGCAGTACCT GGAGCAGGCT ATGCTTCCCG TGAGCTACAA GTTCCCGAAAGGAGCTGATT ACAGCGAACT CCCCACCTAC CCAAAGCGCC CGCACTTCGA CCAGGCAGCGAAGCACCCCA GCACGCAGGT TGTCATCTTC GACGGCTGCC CCAACGACCC TTATCACCCGACCTCGATGC CCATCTACCA AACCGCGACC TTTGTGCAGC CCGACATCGA GGAGTTCGGTCCGTACGACT ACAGCCGTAG CGGCAACCCG ACTCGCACGG CCATCGAGAC CCTCGTCGCCAACCTCGAGG GTGCCCACGC CGCCTTCGCT TTTTCGTCCG GCATGGCGGC ACTGCAGACGCTCGTGACAA CGCTGAACGC TGGCGACACC ATCCTCGCCA GTAGTGACCT TTACGGGGGCATGCACCGTC TCCTGACACA AATCACCTCG TGCCTCGGGA TGACCGTTGT GTTTGTGCCGATGTGGGACA CTGAGGCGGT GCGCCAAGCG CTCGTCGATA ACCCAGCGGC GAAGCTGCTGCACATGGAGA GCCCCACGAA CCCACTGATG CGCATTGTCG ACATCCGTGC TGTCTGTGAGGCTGCCCACG CGCACAACGT AAAGGTGTCC ATCGACAACA CTATGATGTC TCCGCTCCGCTGCACCCCGC TCGCCCTGGG CTGCGACTAC TCCATCCACA GCGCCACCAA GTTTCTGTGCGGCCACAGCG ACACCATGTG CGGTCTTATC TGCGTGAAGT CTGAGGAGGA TGTGAAGCGGGTGGCCTTTC TGCAGAACGC GCAGGGAAAC GCCCTCGCCC CGTTTGACTG CTGGCTGCTCCTGCGTGGCA TCCGCACGCT GTCCATTCGC GTCGAGAAGC AGGAGCTGAA CGCCGTCGCGGTGGCGCTGT TCCTGTCGCG TCAGAGCCAC GTCGTGTCGC GCCTGCACTA CGCTGGTCTGAACCCCGCAA CGTCCCCCCA GATCTCTTCC TTAACTGAGG AGAACTTCTA CCTGCACCGCTCGCAGACCT CCGGCCCTGG CAGCGTGCTC TCCATTGAGA CCGGCAGCGT CAAGCGCTCCAACGCGTTTG TACGGGCCTG CAAGCTGTTC AAGCTCACCG TCAGCTTTGG AAGCTGCAACTCGCTGGTTG AAATGCCCTG CCTCCTCTCG CACGCATCCA TCCCGAAGGA GAAGCGCACACTTCCAGTTG ACCTGATCCG ACTGTCCGTG GGTATCGAAC AGATCGAGGA TATACTTGCGGACCTAACGC AGGCTATCGC CGCCGCGGTC GCCATCCATG ACAATGACGG GTCCTGCTGA |
| Cystathionine beta-lyase-like protein Q4QFT8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | |
| Biochemical Pathway | amino acid metabolism |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | cystathionase isoform 1 [Homo sapiens] | 38 | 1e-65 | 247 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| metB cystathionine gamma-synthase | Haemophilus influenzae | 37% | 1e-63 | 238 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00677 | Cys/Met metabolism enzymes pyridoxal-phosphate attachment site | 249-263; | PS00868 | |
| Acylation | N-myristoylation site | 41-46; 124-129; 247-252; 339-344; 367-372; 397-402; | PS00008 | |
| Glycosylation | N-glycosylation site | 13-16; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 18-21; 113-116; 274-277; 350-353; 401-404; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 35-37; 308-310; 376-378; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 95-109; | PS00003 | |
| Cystathionine beta-lyase-like protein [Q4QFT8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1ibjC | |
| Percent Identity | 48% | |
| Target Region | 62-454 | |
| Template Region | 85-380 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| TYR | 97 | Sidechain |
| ASP | 171 | Sidechain |
| LYS | 196 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 3812-32-6 | CARBONATE ION | 60.009 | C O3 | [O-]C([O-])=O | 1ibj |
| 54-47-7 | PYRIDOXAL-5'-PHOSPHATE | 247.142 | C8 H10 N O6 P | O=P(O)(O)OCc1cnc(c(O)c1C=O)C | 1ibj |
| 14808-79-8 | SULFATE ION | 96.063 | O4 S   | [O-]S([O-])(=O)=O | 1ibj |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/574535/Q4QFT8.pdb 2.0 390 = residues | | = | *| Ramachandran plot: 90.5% core 8.4% allow 0.6% gener 0.6% = disall | | = | *| All Ramachandrans: 13 labelled residues (out of 388) = | +| Chi1-chi2 plots: 4 labelled residues (out of 205) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 17.8 Bad contacts: = 11 | *| Bond len/angle: 5.9 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | | G-factors Dihedrals: 0.02 Covalent: -0.12 Overall: = -0.03 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | | M/c bond angles: 94.9% within limits 5.1% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |