| Serine hydroxymethyltransferase, putative [Q4QFK2] | |
|---|---|
| Systematic Name | LmjF.14.1320 [Leishmania major] |
| Gene Name | SHMT-S |
| Molecular Weight | 50641 Da |
| Protein Sequence Size | 465 |
| Function | |
| Charge | 3 |
| Isoelectric Point | 6.8894 pH |
| Description | Serine hydroxymethyltransferase, putative (EC 2.1.2.1). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 2.1.2.1 |
| Sequence | >tr|Q4QFK2|Q4QFK2_LEIMA Serine hydroxymethyltransferase, putative (EC 2.1.2.1) - Leishmania major. ASLIPTLTEQDPELANMIELEMGRQFRGLEMIASENLTSKAVLECLGSALTNKYAEGEPG NRYYGGTVFVDMVENLAKKRALAAFGLDPGEWGVNVQPYSGSPANFAVYTALLEPHSRIM GLDLPSGGHLTHGFYTPKKKVSATSIYFESFPYHVKEDGLIDYDALESVALVFRPKMIIT GASAYARDFDYERFRHVCDEVGSLLFMDMAHTAGLIAGGVLKSPFPYADVVTTTTHKSLR GPRAGMIFYRKKDRQGKPTDHESRINQAVFPGCQGGPHEHQIAAIATQMREVCSQEWKAY ARQVQSNARALAAALSSKGHVFVSGGTDNHLLLWNVRVHGLTGSKVEKLLDAVSISVNKN TIPGDKSAMTPGGIRVGTLALTSRGMVEADMSTVAEFLDRAIVLAKQIQAAMNAVKLSDF VEALQTHAGAAALRKDVEAFATTFAMPSFDVERIKYKDGLPEEQ |
| DNA Sequence | >LmjF14.1320 |||serine hydroxymethyltransferase, putative|Leishmania major|chr 14|||Manual ATGGCGTCGC TCATTCCCAC CCTGACTGAG CAGGACCCGG AGCTGGCGAA CATGATCGAGCTTGAGATGG GCCGTCAGTT CCGCGGTTTG GAGATGATCG CCTCGGAGAA CCTGACGTCCAAGGCGGTGC TGGAGTGCCT CGGTTCTGCC CTGACGAACA AGTACGCTGA GGGCGAGCCTGGCAACCGCT ACTATGGCGG CACTGTGTTC GTTGACATGG TCGAAAACCT CGCCAAGAAGCGTGCCCTGG CCGCCTTCGG CCTCGACCCG GGAGAGTGGG GGGTCAATGT GCAGCCCTACAGCGGGTCAC CGGCAAACTT CGCCGTCTAC ACTGCTCTGC TGGAGCCGCA CAGCCGCATCATGGGCCTCG ACCTGCCCAG CGGCGGCCAC CTCACCCACG GCTTCTACAC GCCAAAGAAGAAGGTGAGCG CAACGTCTAT CTACTTCGAG TCCTTTCCAT ACCATGTCAA GGAGGACGGCCTGATCGACT ACGACGCTCT CGAGTCCGTC GCGCTGGTGT TCCGACCTAA GATGATCATCACCGGTGCCT CGGCCTACGC GCGTGACTTC GACTACGAGC GGTTCCGTCA CGTCTGCGACGAGGTGGGCA GTCTTCTGTT CATGGACATG GCCCACACGG CCGGTCTAAT TGCCGGTGGCGTGCTCAAGT CGCCGTTTCC GTATGCAGAT GTGGTGACGA CGACGACTCA CAAGTCCCTGCGCGGGCCGC GTGCTGGCAT GATCTTCTAT CGCAAGAAGG ATCGCCAGGG CAAGCCTACGGACCACGAGA GCCGCATCAA TCAGGCAGTT TTCCCTGGTT GCCAGGGCGG TCCGCACGAGCACCAAATTG CCGCCATCGC CACACAGATG CGGGAGGTGT GCAGCCAGGA GTGGAAGGCATACGCCAGGC AAGTGCAGAG CAACGCCCGT GCATTGGCAG CCGCGCTAAG CTCCAAGGGCCACGTGTTCG TTTCCGGTGG CACGGACAAC CACCTGCTGC TGTGGAATGT GCGGGTGCACGGCCTGACCG GTAGCAAGGT CGAAAAGCTA CTCGACGCCG TCTCCATCTC CGTCAACAAGAACACTATCC CTGGCGACAA GAGTGCCATG ACGCCGGGCG GCATTCGCGT TGGCACTCTGGCGCTGACTT CTCGCGGCAT GGTGGAAGCC GACATGAGCA CCGTCGCTGA GTTCCTTGACCGCGCCATTG TGCTGGCCAA GCAAATCCAG GCAGCGATGA ACGCGGTGAA GCTGAGCGACTTTGTCGAGG CGCTGCAGAC GCACGCCGGC GCGGCCGCCC TCCGCAAGGA TGTGGAGGCGTTCGCGACCA CCTTTGCGAT GCCCTCCTTT GATGTGGAGC GTATCAAGTA CAAGGACGGTCTGCCCGAAG AGCAGTGA |
| Serine hydroxymethyltransferase, putative Q4QFK2] | |
|---|---|
| Metabolite Information | |
| Molecular Function | glycine hydroxymethyltransferase activity |
| Biochemical Pathway | L-serine metabolism; glycine metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K00600 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | serine hydroxymethyltransferase 1 (soluble) isoform 1 [Homo sapiens] | 54 | 1e-134 | 476 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| glyA serine hydroxymethyltransferase | Bacillus subtilis | 47% | 5e-91 | 328 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00090 | Serine hydroxymethyltransferase pyridoxal-phosphate attachment site | 230-246; | PS00096 | |
| Acylation | N-myristoylation site | 48-53; 87-92; 102-107; 128-133; 215-220; 326-331; 341-346; 374-379; 386-391; | PS00008 | |
| Glycosylation | N-glycosylation site | 37-40; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 140-143; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 7-10; 9-12; 260-263; 345-348; 394-397; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 39-41; 52-54; 137-139; 236-238; 239-241; 317-319; 383-385; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 157-171; | PS00003 | |
| Serine hydroxymethyltransferase, putative [Q4QFK2] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1bj4A | |
| Percent Identity | 54% | |
| Target Region | 1-450 | |
| Template Region | 11-470 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| GLU | 57 | Sidechain |
| THR | 235 | Sidechain |
| LYS | 238 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Pyridoxal phosphate | |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 54-47-7 | PYRIDOXAL-5'-PHOSPHATE | 247.142 | C8 H10 N O6 P | O=P(O)(O)OCc1cnc(c(O)c1C=O)C | 1bj4 |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/386829/Q4QFK2.pdb 2.0 450 = residues | | = | *| Ramachandran plot: 91.8% core 6.4% allow 0.8% gener 1.0% = disall | | = | +| All Ramachandrans: 13 labelled residues (out of 448) = | +| Chi1-chi2 plots: 2 labelled residues (out of 245) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 3 | *| Bond len/angle: 8.3 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.13 Covalent: -0.12 Overall: = 0.04 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | *| M/c bond angles: 94.8% within limits 5.2% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |