| Myo-inositol-1-phosphate synthase [Q4QFJ8] | |
|---|---|
| Systematic Name | LmjF.14.1360 [Leishmania major] |
| Gene Name | INO1 |
| Molecular Weight | 58301 Da |
| Protein Sequence Size | 526 |
| Function | |
| Charge | -3.5 |
| Isoelectric Point | 5.9956 pH |
| Description | Myo-inositol-1-phosphate synthase. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QFJ8|Q4QFJ8_LEIMA Myo-inositol-1-phosphate synthase - Leishmania major. PAVHVKSDKVKYTPEVIESLYDYATTRVRRNTDGTVSVEPTKCQLLFRTARQVPRVGVML VGWCGNNGTTVTAGILANKLGLKWRTRHGMQSANYFGSITQSSTINLGMTRDMDEVFVPL KDLVPMVSPDNLVIGGWDCNNMNLGDAMRRAAVLDVQVQDALYDHMKKLCPLPAIFDIDF VAANQKDRANNVLDTCDRWEAVEQVRKDIRDFKSANELEKVIVLWTANTERFSEHRDGVH DTAENLLAAVKRNESELAPSTLYAMAAVLEKCSFINGAPQNTLCPGLVEMAREAKVFVGG DDFKSGQTKMKSALVEFFVGAGIKPECIASYNHLGNNDGYNLSSHKQFCSKEITKSNVVD DMIKSNQVLFPEGARKPDHCIVIKYIPYVGDSKRALDEYTFSIFMGGQQTVVLHNTCEDS LLAAPLIIDLIVLTELMERVTISASDDTQTPPPASFEHMETVLSILSYLLKAPAVPEGTP VVNALNRQRAAIENVLRAMIGLPPDSNMLLECRVPFIREEHINGK |
| DNA Sequence | >LmjF14.1360 |INO1||myo-inositol-1-phosphate synthase|Leishmania major|chr 14|||Manual ATGCCGGCAG TACACGTGAA GAGCGACAAA GTGAAGTACA CGCCTGAGGT TATCGAGTCGCTGTATGACT ATGCCACGAC GCGCGTGAGA CGCAACACAG ACGGCACGGT GTCGGTGGAGCCGACGAAGT GCCAACTGCT GTTCCGCACG GCGCGCCAGG TGCCGCGCGT GGGCGTGATGCTGGTCGGCT GGTGCGGCAA CAACGGCACG ACAGTGACAG CGGGTATCCT TGCGAACAAGCTGGGCCTGA AGTGGCGCAC GCGGCACGGC ATGCAGTCGG CCAATTATTT TGGCTCCATCACTCAGTCCA GCACCATCAA CCTGGGCATG ACGCGCGACA TGGACGAGGT CTTCGTGCCGCTCAAGGACT TGGTGCCGAT GGTGTCACCC GACAATCTGG TTATCGGCGG GTGGGACTGCAACAACATGA ACCTCGGCGA CGCGATGCGG CGCGCCGCCG TTCTGGATGT GCAGGTGCAGGACGCGCTCT ACGATCATAT GAAAAAGTTG TGCCCGCTGC CGGCCATCTT CGACATTGACTTCGTCGCGG CAAACCAGAA GGATCGCGCG AACAATGTGC TCGACACGTG CGACCGCTGGGAGGCAGTGG AGCAGGTGCG CAAGGACATC CGCGACTTCA AGTCGGCCAA CGAGCTGGAAAAGGTGATTG TGCTGTGGAC TGCTAATACA GAGCGCTTCA GCGAGCACCG CGACGGCGTCCACGACACGG CCGAGAACCT GCTGGCGGCG GTGAAGCGCA ACGAGTCGGA GTTGGCGCCGTCCACACTGT ACGCGATGGC AGCCGTTCTC GAGAAGTGCA GCTTCATCAA CGGCGCGCCGCAAAACACGC TGTGCCCCGG GCTGGTGGAG ATGGCGCGCG AGGCGAAGGT CTTCGTCGGCGGTGACGACT TCAAGAGCGG TCAGACCAAG ATGAAGAGCG CCCTGGTGGA GTTCTTTGTCGGCGCCGGCA TCAAGCCCGA GTGCATCGCC AGCTACAACC ACCTCGGCAA CAACGATGGCTACAACTTGT CCAGCCACAA GCAGTTCTGC TCGAAAGAGA TTACCAAGAG CAACGTGGTGGACGACATGA TCAAGTCCAA CCAGGTGCTC TTCCCCGAAG GTGCGCGAAA GCCGGACCACTGCATCGTCA TCAAGTACAT TCCCTACGTT GGGGACAGCA AGCGCGCACT AGACGAGTACACCTTCTCCA TATTCATGGG TGGCCAGCAG ACAGTCGTGC TGCACAACAC CTGCGAGGACTCGCTACTTG CAGCGCCGCT CATCATTGAC CTTATCGTGC TCACCGAGCT CATGGAGCGCGTCACGATCA GCGCCAGCGA CGACACGCAG ACGCCGCCGC CAGCGTCCTT CGAGCATATGGAGACGGTGC TGTCCATTCT GTCCTACCTG CTCAAGGCGC CTGCCGTGCC AGAGGGCACGCCGGTCGTTA ACGCGCTCAA TCGCCAGAGG GCGGCCATCG AGAACGTGCT GCGTGCCATGATTGGGCTTC CGCCGGACAG CAACATGCTG CTCGAGTGCC GCGTTCCCTT CATACGCGAGGAGCACATCA ACGGCAAGTG A |
| Myo-inositol-1-phosphate synthase Q4QFJ8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | inositol-3-phosphate synthase activity |
| Biochemical Pathway | myo-inositol biosynthesis; phospholipid biosynthesis |
| Regulatory Pathway | |
| KEGG Pathways | K01858 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | myo-inositol 1-phosphate synthase A1 [Homo sapiens] | 58 | 1e-167 | 587 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YJL153c myo-inositol-1-phosphate synthase | Saccharomyces cerevisiae | 48% | 1e-140 | 491 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| Acylation | N-myristoylation site | 66-71; 69-74; 75-80; 90-95; 136-141; 239-244; 278-283; 336-341; 407-412; | PS00008 | |
| Glycosylation | N-glycosylation site | 68-71; 254-257; 342-345; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 30-33; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 20-23; 215-218; 417-420; 444-447; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 8-10; 26-28; 50-52; 230-232; 345-347; 393-395; | PS00005 | |
| Myo-inositol-1-phosphate synthase [Q4QFJ8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1vkoA | |
| Percent Identity | 51% | |
| Target Region | 1-526 | |
| Template Region | 11-511 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 16887-00-6 | CHLORIDE ION | 35.453 | Cl | [Cl-] | 1vko |
| 20461-54-5 | IODIDE ION | 126.904 | I | [I-] | 1vko |
| 24203-36-9 | POTASSIUM ION | 39.098 | K | [K+] | 1vko |
| NICOTINAMIDE-ADENINE-DINUCLEOTIDE | 663.425 | C21 H27 N7 O14 P2 | c1cc(c[n+](c1)C2C(C(C(O2)COP(=O)([O-])OP(=O)(O)OCC3C(C(C(O3)n4cnc5c4ncnc5N)O)O)O)O)C(=O)N | 1vko | |
| PYROPHOSPHATE 2- | 175.959 | H2 O7 P2 | [O-]P(=O)(O)OP([O-])(=O)O | 1vko | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/54772/Q4QFJ8.pdb 2.0 526 = residues | | = | *| Ramachandran plot: 92.3% core 7.1% allow 0.4% gener 0.2% = disall | | = | +| All Ramachandrans: 9 labelled residues (out of 524) = | +| Chi1-chi2 plots: 5 labelled residues (out of 306) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 5.7 Bad contacts: = 7 | *| Bond len/angle: 12.8 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.03 Covalent: -0.20 Overall: = -0.06 | | = | | M/c bond lengths: 99.0% within limits 1.0% highlighted = | *| M/c bond angles: 93.6% within limits 6.4% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |