| Cytochrome-b5 reductase, putative [Q4QFH9] | |
|---|---|
| Systematic Name | LmjF.15.0050 [Leishmania major] |
| Gene Name | LMJF_15_0050 |
| Molecular Weight | 31512 Da |
| Protein Sequence Size | 279 |
| Function | |
| Charge | 11 |
| Isoelectric Point | 10 pH |
| Description | Cytochrome-b5 reductase, putative (EC 1.6.2.2). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 1.6.2.2 |
| Sequence | >tr|Q4QFH9|Q4QFH9_LEIMA Cytochrome-b5 reductase, putative (EC 1.6.2.2) - Leishmania major. WSTRQQSERLAATCAKKPVDTFSPDAYKPFKLISSRYESHDTRRFYFALDSPDDSFYMPV ASCIIAKYTDADGKDVARPYTPISSNSTKGHFELVVKKYPKGKMGNYLFSMQPGDELLVK GPFEKFAYKPNMWKHVGMIAGGTGIAPMYQVLRAVLENPRDKTNISLIYANNQRRDILLA NELIEMQKVYTNFNMYLTLLEVPHRWLGGIGYVNSAMVTTFMPKPGEKNTKILVCGPPPM MQAISGDKLFEPGKPPQQGQVGGLLKTLGYTEEQVFKY |
| DNA Sequence | >LmjF15.0050 |||cytochrome-b5 reductase, putative|Leishmania major|chr 15|||Manual ATGTGGAGTA CCAGGCAGCA GTCGGAGCGC TTAGCGGCCA CCTGCGCCAA GAAACCAGTGGATACGTTCT CCCCCGATGC GTACAAGCCA TTTAAGCTGA TTTCCTCTCG GTACGAGTCGCACGATACGC GCCGCTTTTA CTTTGCCCTC GATAGCCCCG ATGATAGCTT CTACATGCCAGTTGCTTCGT GCATCATCGC GAAGTACACC GATGCAGACG GCAAGGACGT TGCTCGGCCATACACTCCCA TCTCCTCTAA TAGCACCAAG GGCCACTTTG AACTTGTTGT GAAGAAGTATCCAAAAGGAA AAATGGGCAA CTATCTGTTC TCCATGCAAC CAGGCGACGA ACTGCTCGTCAAGGGCCCTT TTGAGAAGTT TGCGTACAAG CCCAACATGT GGAAGCACGT GGGCATGATCGCCGGTGGTA CCGGCATCGC TCCAATGTAC CAGGTGCTTC GTGCCGTGCT AGAGAATCCCAGAGACAAGA CCAACATTTC TCTCATCTAT GCCAACAACC AGCGCCGCGA CATTCTGCTTGCTAACGAAC TGATTGAGAT GCAGAAGGTC TACACCAACT TCAACATGTA TTTGACCCTTCTGGAGGTGC CGCACCGATG GCTAGGCGGT ATCGGCTATG TGAATAGTGC CATGGTAACCACCTTTATGC CGAAGCCGGG GGAGAAGAAC ACGAAGATCC TCGTATGTGG CCCCCCTCCGATGATGCAGG CCATCAGCGG CGACAAGCTG TTTGAGCCTG GCAAGCCGCC CCAGCAGGGCCAGGTAGGCG GCCTGCTGAA AACGCTAGGC TACACGGAGG AGCAGGTCTT CAAGTACTGA |
| Cytochrome-b5 reductase, putative Q4QFH9] | |
|---|---|
| Metabolite Information | |
| Molecular Function | oxidoreductase activity |
| Biochemical Pathway | electron transport |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | cytochrome b5 reductase isoform 2 [Homo sapiens] | 43 | 8e-43 | 171 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| SA0272 hypothetical protein, similar to transmembrane protein Tmp7 | Staphylococcus aureus | 22% | 2.2 | 26.9 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| Acylation | N-myristoylation site | 138-143; 260-265; 264-269; | PS00008 | |
| Glycosylation | N-glycosylation site | 87-90; 165-168; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 36-39; 52-55; 70-73; 199-202; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 3-5; 8-10; 35-37; 43-45; 88-90; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 121-129; | PS00007 | |
| Cytochrome-b5 reductase, putative [Q4QFH9] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2eixB | |
| Percent Identity | 44% | |
| Target Region | 18-279 | |
| Template Region | 39-243 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| TYR | 64 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 16426-55-4 | FLAVIN-ADENINE DINUCLEOTIDE | 785.55 | C27 H33 N9 O15 P2 | O=C2C3=Nc1cc(c(cc1N(C3=NC(=O)N2)CC(O)C(O)C(O)COP(=O)(O)OP(=O)(O)OCC6OC(n5cnc4c(ncnc45)N)C(O)C6O)C)C | 2eix |
| 8013-25-0 | GLYCEROL | 92.094 | C3 H8 O3 | OCC(O)CO | 2eix |
| 20461-54-5 | IODIDE ION | 126.904 | I | [I-] | 2eix |
| 7440-23-5 | SODIUM ION | 22.99 | Na | [Na+] | 2eix |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/43786/Q4QFH9.pdb 2.0 262 = residues | | = | +| Ramachandran plot: 96.3% core 3.2% allow 0.5% gener 0.0% = disall | | = | +| All Ramachandrans: 4 labelled residues (out of 260) = | +| Chi1-chi2 plots: 3 labelled residues (out of 159) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | +| Residue properties: Max.deviation: 5.5 Bad contacts: = 2 | +| Bond len/angle: 4.6 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.05 Covalent: -0.18 Overall: = -0.03 | | = | | M/c bond lengths: 98.5% within limits 1.5% highlighted = | | M/c bond angles: 92.8% within limits 7.2% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |