| Heat shock protein HslVU, ATPase subunit HslU, putative [Q4QFH5] | |
|---|---|
| Systematic Name | LmjF.15.0090 [Leishmania major] |
| Gene Name | LMJF_15_0090 |
| Molecular Weight | 47495 Da |
| Protein Sequence Size | 423 |
| Function | |
| Charge | 0 |
| Isoelectric Point | 6.5 pH |
| Description | Heat shock protein HslVU, ATPase subunit HslU, putative. |
| Subcellular Location | cytoplasm; HslUV protease complex[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QFH5|Q4QFH5_LEIMA Heat shock protein HslVU, ATPase subunit HslU, putative - Leishmania major. RPRELMKELDRYIVGQSEAKKAVSVALRNRWRRHQVPSDIREEIAPKNILMIGPTGVGKT EIARRLAKLVDAPFVKVEATKFTEVGFHGRDVESIIEDLYKASLSQTKQNIRRQHEEEAK LKAEDRILKSLAGVSDGFREHLRSGALDDIEVMVELQEKKEKPKTASGEGVFISLDIPSM MGGQRQQTVKKVMKIKDAFPAVLQEELDKMMDTEDVTAEALRACEEDGIVVIDEIDKIVT AAGGYKGHQASAEGVQQDLLPLVEGTTVSTKFNVQVKTDKILFICSGAFHSVKPSDMLAE LQGRLPIRVELQQLSKEDFHRIITEPRFNLIAQHKAMMATEGVDLIFEDDALWEIASIAA YINSTVQNIGARRLITITEKVVEEISFEGPERKSEKFVIDAAYVKKAVDKMVKKVDIKKF IL |
| DNA Sequence | >LmjF15.0090 |||heat shock protein HslVU, ATPase subunit HslU, putative|Leishmania major|chr 15|||Manual ATGCGTCCAC GCGAGCTGAT GAAGGAGCTA GACCGCTACA TTGTGGGTCA GAGCGAGGCGAAGAAAGCTG TGTCGGTGGC GCTGCGCAAT CGCTGGCGCC GCCACCAGGT GCCGAGCGACATTCGCGAGG AAATCGCGCC AAAGAATATT CTGATGATTG GCCCGACCGG CGTGGGCAAGACGGAGATTG CCCGCCGCCT CGCCAAGCTC GTAGATGCTC CGTTCGTCAA GGTGGAGGCGACCAAGTTCA CGGAGGTCGG TTTCCACGGC CGCGACGTGG AGAGCATCAT CGAGGACCTCTATAAGGCCT CTTTGTCGCA GACCAAGCAG AACATCCGTC GCCAGCATGA GGAAGAGGCGAAGTTGAAGG CGGAGGACCG CATTCTTAAG TCTTTGGCCG GCGTGTCAGA CGGCTTCCGCGAGCACCTGC GCAGCGGGGC ATTGGACGAC ATTGAGGTCA TGGTAGAGCT TCAAGAGAAGAAAGAGAAAC CCAAGACAGC GAGCGGCGAG GGCGTCTTCA TTTCTCTCGA CATCCCTTCCATGATGGGCG GGCAGCGCCA GCAGACGGTG AAGAAGGTGA TGAAGATCAA GGACGCCTTCCCGGCTGTGC TGCAGGAGGA GCTGGACAAG ATGATGGACA CCGAGGACGT CACGGCTGAGGCGCTGCGCG CCTGCGAGGA GGACGGCATC GTTGTCATTG ACGAGATCGA CAAGATCGTGACGGCGGCGG GCGGGTACAA GGGTCACCAG GCTTCCGCCG AGGGCGTCCA GCAGGATCTGCTACCTTTGG TAGAGGGCAC CACGGTGTCG ACGAAGTTTA ATGTGCAGGT GAAGACGGACAAGATCTTGT TTATCTGCAG CGGCGCCTTC CACAGCGTTA AGCCGTCCGA CATGCTGGCGGAGCTGCAGG GTCGTCTGCC CATCCGCGTC GAGCTCCAGC AGCTGAGCAA GGAGGACTTTCACCGCATCA TCACCGAGCC TCGTTTCAAC CTTATTGCGC AACACAAGGC GATGATGGCTACAGAGGGTG TCGACCTTAT CTTTGAGGAC GACGCACTCT GGGAGATTGC TAGCATCGCCGCGTATATCA ACTCGACAGT GCAGAACATC GGTGCCCGCC GCCTCATCAC AATCACGGAGAAGGTTGTTG AGGAGATCAG CTTCGAGGGG CCGGAGCGGA AGAGCGAGAA GTTCGTCATTGACGCCGCTT ACGTCAAGAA GGCCGTGGAC AAGATGGTCA AGAAAGTGGA CATCAAGAAGTTCATCTTGT GA |
| Heat shock protein HslVU, ATPase subunit HslU, putative Q4QFH5] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; ATPase activity; GTP binding; HslUV protease activity; RNA binding; nucleoside-triphosphatase activity; nucleotide binding; protein binding |
| Biochemical Pathway | ATP-dependent proteolysis; SRP-dependent cotranslational protein targeting to membrane |
| Regulatory Pathway | |
| KEGG Pathways | K03667 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | ClpX caseinolytic protease X homolog [Homo sapiens] | 23 | 0.0000000000003 | 74 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| clpX ATP-dependent Clp protease ATP-binding subunit (class III heat-shock protein) | Streptococcus pneumoniae | 28% | 2e-30 | 127 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 54-61; | PS00017 | |
| Acylation | N-myristoylation site | 16-21; 57-62; 134-139; 171-176; 244-249; 266-271; 288-293; | PS00008 | |
| Glycosylation | N-glycosylation site | 364-367; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 95-98; 316-319; 377-380; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 189-191; 270-272; 279-281; 292-294; 379-381; 395-397; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 397-404; | PS00007 | |
| Heat shock protein HslVU, ATPase subunit HslU, putative [Q4QFH5] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1yyfB | |
| Percent Identity | 48% | |
| Target Region | 1-423 | |
| Template Region | 1-408 | |
| Domain Information | ||
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| Domains | Start | End |
| Active Site Information | ||
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| Residue | Active Site Number | Functional Part |
| Co-Factor | |
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| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 58-64-0 | ADENOSINE-5'-DIPHOSPHATE | 507.181 | C10 H16 N5 O13 P3 | O=P(O)(O)OP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 1yyf |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1623666/Q4QFH5.pdb 2.0 423 = residues | | = | *| Ramachandran plot: 89.6% core 7.6% allow 2.1% gener 0.8% = disall | | = | *| All Ramachandrans: 20 labelled residues (out of 421) = | +| Chi1-chi2 plots: 1 labelled residues (out of 270) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 19.5 Bad contacts: = 12 | *| Bond len/angle: 6.2 Morris et al class: 1 = 1 3 | | = | | G-factors Dihedrals: -0.09 Covalent: -0.28 Overall: = -0.15 | | = | | M/c bond lengths: 98.8% within limits 1.2% highlighted = | | M/c bond angles: 91.8% within limits 8.2% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |