| Dihydroorotate dehydrogenase, putative [Q4QEW7] | |
|---|---|
| Systematic Name | LmjF.16.0530 [Leishmania major] |
| Gene Name | DHODH |
| Molecular Weight | 34662 Da |
| Protein Sequence Size | 320 |
| Function | |
| Charge | -2 |
| Isoelectric Point | 5.7446 pH |
| Description | Dihydroorotate dehydrogenase, putative. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QEW7|Q4QEW7_LEIMA Dihydroorotate dehydrogenase, putative - Leishmania major. SLQVNLLNNTFANPFMNAAGVMCTTTEELVAMTESASGSLVSKSCTPALREGNPTPRYQA LPLGSINSMGLPNNGFDFYLAYAAEQHDYGKKPLFLSMSGLSMRENVEMCKRLAAVATEK GVILELNLSCPNVPGKPQVAYDFDAMRQCLTAVSEVYPHSFGVKMPPYFDFAHFDAAAEI LNEFPKVQFITCINSIGNGLVIDAETESVVIKPKQGFGGLGGRYVLPTALANINAFYRRC PGKLIFGCGGVYTGEDAFLHVLAGASMVQVGTALQEEGPSIFERLTSELLGVMAKKRYQT LDEFRGKVRTLDGTAESTR |
| DNA Sequence | >LmjF16.0530 |||dihydroorotate dehydrogenase, putative|Leishmania major|chr 16|||Manual ATGAGCCTTC AGGTGAATCT TCTCAACAAC ACGTTTGCCA ACCCGTTCAT GAACGCGGCTGGCGTGATGT GCACCACGAC GGAAGAGCTG GTGGCCATGA CCGAGTCGGC GAGTGGCTCTCTGGTCTCGA AGAGTTGCAC CCCCGCTCTC CGCGAGGGCA ATCCGACTCC GCGCTACCAGGCCCTGCCAC TTGGCAGCAT CAACTCGATG GGCCTGCCAA ACAACGGGTT CGACTTCTATCTGGCCTACG CCGCGGAGCA GCACGACTAC GGCAAGAAGC CGCTCTTTCT CTCCATGTCCGGCTTGTCAA TGAGGGAGAA TGTTGAGATG TGCAAGCGCC TTGCCGCCGT GGCGACAGAGAAGGGCGTCA TCCTGGAGCT GAATCTCTCC TGCCCCAACG TTCCTGGCAA GCCGCAGGTGGCCTACGACT TCGACGCGAT GCGGCAGTGC TTGACGGCGG TCTCTGAGGT TTACCCGCACTCGTTTGGTG TGAAGATGCC GCCGTACTTC GACTTTGCCC ACTTTGATGC CGCCGCCGAGATCCTCAACG AGTTCCCGAA GGTGCAGTTC ATTACCTGCA TCAACAGCAT CGGTAACGGCCTCGTGATCG ACGCGGAGAC GGAGTCCGTG GTGATCAAGC CGAAGCAGGG CTTTGGCGGCCTCGGTGGTC GCTACGTCCT CCCCACGGCG CTGGCGAACA TCAACGCGTT CTACCGCCGTTGCCCGGGCA AGCTCATCTT TGGCTGCGGT GGCGTGTACA CCGGTGAAGA CGCCTTCCTGCACGTGCTCG CTGGCGCCTC CATGGTGCAG GTCGGCACGG CTCTGCAGGA GGAGGGACCCTCCATCTTCG AGCGGCTCAC GTCGGAGCTG CTGGGCGTGA TGGCGAAGAA GCGCTACCAGACGCTGGACG AGTTCCGCGG CAAGGTCAGG ACACTGGATG GAACGGCCGA GAGCACGCGCTAA |
| Dihydroorotate dehydrogenase, putative Q4QEW7] | |
|---|---|
| Metabolite Information | |
| Molecular Function | dihydroorotate dehydrogenase activity |
| Biochemical Pathway | de novo' pyrimidine base biosynthesis |
| Regulatory Pathway | |
| KEGG Pathways | K00226 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | dihydroorotate dehydrogenase isoform 1 precursor [Homo sapiens] | 27 | 0.000000000005 | 69 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| fbaA Fructose-bisphosphate aldolase class II (EC 4.1.2.13) | Escherichia coli MG1655 | 37% | 0.11 | 31.6 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| Acylation | N-myristoylation site | 21-26; 39-44; 65-70; 71-76; 219-224; 250-255; 251-256; 314-319; | PS00008 | |
| Amidation | Amidation site | 90-93; | PS00009 | |
| Glycosylation | N-glycosylation site | 9-12; 128-131; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 25-28; 26-29; 103-106; 254-257; 281-284; 301-304; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 56-58; 103-105; 119-121; 318-320; | PS00005 | |
| Dihydroorotate dehydrogenase, putative [Q4QEW7] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2djxB | |
| Percent Identity | 75% | |
| Target Region | 1-320 | |
| Template Region | 1-313 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| LYS | 44 | Sidechain |
| CYS | 131 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 6184-17-4 | FLAVIN MONONUCLEOTIDE | 456.344 | C17 H21 N4 O9 P | O=P(O)(O)OCC(O)C(O)C(O)CN2c3cc(c(cc3N=C1C2=NC(=O)NC1=O)C)C | 2djx |
| COBALT HEXAMMINE ION | 161.116 | Co H18 N6 | [NH3][Co+3]([NH3])([NH3])([NH3])([NH3])[NH3] | 2djx | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1699178/Q4QEW7.pdb 2.0 320 = residues | | = | | Ramachandran plot: 95.2% core 4.8% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 2 labelled residues (out of 318) = | +| Chi1-chi2 plots: 1 labelled residues (out of 173) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.9 Bad contacts: = 0 | *| Bond len/angle: 5.2 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | | G-factors Dihedrals: 0.04 Covalent: -0.11 Overall: = -0.01 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | | M/c bond angles: 94.3% within limits 5.7% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |