| ADP-ribosylation factor, putative [Q4QEJ1] | |
|---|---|
| Systematic Name | LmjF.17.0070 [Leishmania major] |
| Gene Name | ARL-1 |
| Molecular Weight | 20791 Da |
| Protein Sequence Size | 187 |
| Function | |
| Charge | -5 |
| Isoelectric Point | 4.7371 pH |
| Description | ADP-ribosylation factor, putative. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QEJ1|Q4QEJ1_LEIMA ADP-ribosylation factor, putative - Leishmania major. GAWLASLKQTLGLLPADRKIRVLMLGLDNAGKTSILYRLHLGDVVTTVPTVGVNLETLQY KNISFEVWDLGGQTGVRPYWRCYFSDTDAVIYVVDSTDRDRMGVAKHELYALLDEDELRK SLLLIFANKQDLPDAASEAEIAEQLGVSSIMNRTWTIVKSSSKTGDGLVEGMDWLVERLR EQGLGA |
| DNA Sequence | >LmjF17.0070 |ARL-1||ADP-ribosylation factor, putative|Leishmania major|chr 17|||Manual ATGGGTGCGT GGCTGGCAAG TCTGAAGCAG ACACTTGGTC TGCTGCCAGC AGACCGCAAAATTCGTGTCT TGATGCTCGG CCTGGACAAT GCGGGGAAGA CTTCCATTCT CTATCGGCTACACCTCGGTG ATGTGGTTAC CACCGTCCCC ACTGTGGGAG TCAACCTAGA AACTCTTCAGTACAAGAACA TCTCCTTCGA AGTGTGGGAC CTTGGCGGCC AAACGGGCGT GCGACCGTATTGGCGTTGCT ACTTCAGCGA CACAGACGCC GTCATCTACG TGGTGGACAG CACTGACAGGGATCGCATGG GCGTCGCCAA GCACGAACTG TACGCTCTCC TAGACGAGGA CGAGCTCAGAAAGAGTCTCT TGCTTATATT TGCCAACAAG CAAGATTTGC CGGACGCCGC CAGCGAGGCAGAGATTGCCG AACAGCTTGG TGTGTCGTCC ATTATGAACC GTACGTGGAC GATTGTAAAAAGCAGTTCAA AAACAGGTGA TGGCCTGGTG GAAGGCATGG ACTGGCTCGT GGAGAGGCTTCGTGAGCAAG GGCTCGGCGC TTAG |
| ADP-ribosylation factor, putative Q4QEJ1] | |
|---|---|
| Metabolite Information | |
| Molecular Function | GTP binding |
| Biochemical Pathway | ER to Golgi vesicle-mediated transport; intracellular protein transport; small GTPase mediated signal transduction |
| Regulatory Pathway | |
| KEGG Pathways | K07942 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | ADP-ribosylation factor-like 1 [Homo sapiens] | 52 | 7e-54 | 206 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YPL218w GTP-binding protein of the ARF family | Saccharomyces cerevisiae | 32% | 3e-20 | 92 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 27-34; | PS00017 | |
| Acylation | N-myristoylation site | 2-7; 13-18; 27-32; 72-77; 168-173; | PS00008 | |
| Glycosylation | N-glycosylation site | 63-66; 153-156; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 86-89; 98-101; 138-141; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 7-9; 98-100; 162-164; | PS00005 | |
| ADP-ribosylation factor, putative [Q4QEJ1] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1r4aD | |
| Percent Identity | 55% | |
| Target Region | 19-187 | |
| Template Region | 16-165 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASN | 12 | Sidechain (GLY 27 In Template) |
| GLN | 56 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | 522.196 | C10 H17 N6 O13 P3 | O=P(O)(O)NP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 1r4a | |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 1r4a |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/168064/Q4QEJ1.pdb 2.0 169 = residues | | = | +| Ramachandran plot: 92.0% core 7.3% allow 0.7% gener 0.0% = disall | | = | +| All Ramachandrans: 5 labelled residues (out of 167) = | +| Chi1-chi2 plots: 1 labelled residues (out of 103) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 2.3 Bad contacts: = 5 | *| Bond len/angle: 6.7 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.01 Covalent: -0.10 Overall: = -0.03 | | = | | M/c bond lengths: 99.6% within limits 0.4% highlighted = | | M/c bond angles: 94.3% within limits 5.7% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |