| Elongation factor 1-alpha [Q4QEI8] | |
|---|---|
| Systematic Name | LmjF.17.0082 [Leishmania major] |
| Gene Name | LMJF_17_0082 |
| Molecular Weight | 49059 Da |
| Protein Sequence Size | 449 |
| Function | |
| Charge | 16.5 |
| Isoelectric Point | 9.453 pH |
| Description | Elongation factor 1-alpha. |
| Subcellular Location | cytoplasm[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QEI8|Q4QEI8_LEIMA Elongation factor 1-alpha - Leishmania major. GKDKVHMNLVVVGHVDAGKSTATGHLIYKCGGIDKRTIEKFEKEAAEIGKASFKYAWVLD KLKAERERGITIDIALWKFESPKSVFTIIDAPGHRDFIKNMITGTSQADAAILMIDSTHG GFEAGISKDGQTREHALLAFTLGVKQMVVCCNKMDDKTVTYAQSRYDEISKEVGAYLKRV GYNPEKVRFIPISGWQGDNMIEKSDNMPWYKGPTLLDALGMLEPPVRPVDKPLRLPLQDV YKIGGIGTVPVGRVETGIMKPGDVVTFAPANVTTEVKSIEMHHEQLAEAQPGDNVGFNVK NVSVKDIRRGNVCGNSKNDPPKEAADFTAQVIVLNHPGQISNGYAPVLDCHTSHIACRFA EIESKIDRRSGKELEKNPKAIKSGDAAIVKMVPQKPMCVEVFNDYAPLGRFAVRDMRQTV AVGIIKGVNKKEGSGGKVTKAAAKASKK |
| DNA Sequence | >LmjF17.0082 |||elongation factor 1-alpha|Leishmania major|chr 17|||Manual ATGGGCAAGG ATAAGGTGCA CATGAACCTT GTGGTCGTCG GCCATGTCGA CGCCGGCAAGTCCACCGCCA CTGGCCACTT GATCTACAAG TGCGGTGGCA TCGACAAGCG CACGATCGAGAAGTTCGAGA AGGAGGCCGC CGAGATCGGC AAGGCGTCCT TCAAGTACGC GTGGGTGCTCGACAAGCTGA AGGCGGAGCG CGAGCGCGGC ATCACGATCG ACATTGCGCT GTGGAAGTTCGAGTCGCCCA AGTCCGTGTT CACGATCATC GATGCGCCCG GCCACCGCGA CTTCATCAAGAACATGATCA CGGGCACGTC GCAGGCGGAC GCCGCCATTC TGATGATCGA CTCGACGCATGGCGGCTTCG AGGCTGGCAT CTCGAAGGAC GGCCAGACCC GCGAGCACGC GCTGCTTGCCTTCACTCTTG GCGTGAAGCA GATGGTGGTG TGCTGCAACA AGATGGACGA CAAGACGGTGACGTACGCGC AGTCGCGCTA CGATGAGATC AGCAAGGAGG TGGGCGCGTA CCTGAAGCGCGTGGGCTACA ACCCGGAGAA GGTGCGCTTC ATCCCGATCT CGGGCTGGCA GGGCGACAACATGATCGAGA AGTCGGACAA CATGCCGTGG TACAAGGGTC CCACGCTGCT GGACGCGCTCGGCATGCTGG AGCCGCCGGT GCGCCCGGTG GACAAGCCGC TGCGCCTGCC CCTGCAGGACGTGTACAAGA TCGGCGGTAT CGGGACGGTG CCCGTGGGCC GCGTGGAGAC CGGCATCATGAAGCCGGGCG ACGTGGTGAC GTTCGCGCCC GCCAACGTGA CGACTGAGGT GAAGTCGATCGAGATGCACC ACGAGCAGCT GGCGGAGGCG CAGCCCGGCG ACAACGTCGG CTTCAACGTGAAGAACGTGT CGGTGAAGGA CATCCGCCGT GGTAACGTGT GCGGCAACTC GAAGAACGACCCGCCGAAGG AGGCGGCCGA CTTCACGGCG CAGGTGATCG TGCTGAACCA CCCCGGCCAGATCAGCAACG GCTATGCGCC GGTGCTGGAC TGCCACACGA GCCACATTGC GTGCCGCTTCGCGGAAATCG AGTCCAAGAT CGACCGCCGC TCCGGCAAGG AGCTGGAGAA GAACCCCAAGGCGATCAAGT CTGGCGATGC CGCGATCGTG AAGATGGTGC CGCAGAAGCC GATGTGCGTGGAGGTGTTCA ACGACTACGC GCCGCTGGGC CGCTTTGCCG TGCGCGACAT GCGCCAGACGGTGGCCGTGG GCATCATCAA GGGCGTGAAC AAGAAGGAGG GCAGCGGCGG TAAGGTGACCAAGGCGGCCG CGAAGGCTTC GAAGAAGTAA |
| Elongation factor 1-alpha Q4QEI8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | GTP binding; calmodulin binding; translation elongation factor activity |
| Biochemical Pathway | calcium-mediated signaling; microtubule-based process; protein biosynthesis; translational elongation |
| Regulatory Pathway | |
| KEGG Pathways | K03231 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | eukaryotic translation elongation factor 1 alpha 2 [Homo sapiens] | 75 | 0 | 707 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YDR172w eukaryotic peptide chain release factor GTP-binding subunit | Saccharomyces cerevisiae | 38% | 7e-85 | 308 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 14-21; | PS00017 | |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 272-275; 302-305; | PS00722 | |
| PDOC00273 | GTP-binding elongation factors signature | 61-76; | PS00301 | |
| Acylation | N-myristoylation site | 105-110; 121-126; 245-250; 311-316; 315-320; 339-344; 424-429; 436-441; | PS00008 | |
| Glycosylation | N-glycosylation site | 272-275; 302-305; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 88-91; 107-110; 165-168; 215-218; 304-307; 317-320; 365-368; 371-374; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 53-55; 82-84; 304-306; 371-373; 447-449; | PS00005 | |
| Elongation factor 1-alpha [Q4QEI8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2b7cA | |
| Percent Identity | 74% | |
| Target Region | 1-442 | |
| Template Region | 5-436 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 17 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1691353/Q4QEI8.pdb 2.0 430 = residues | | = | *| Ramachandran plot: 93.7% core 5.2% allow 0.8% gener 0.3% = disall | | = | +| All Ramachandrans: 8 labelled residues (out of 428) = | +| Chi1-chi2 plots: 1 labelled residues (out of 248) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 3 | *| Bond len/angle: 5.0 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.04 Covalent: -0.09 Overall: = -0.01 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | | M/c bond angles: 94.3% within limits 5.7% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |