| Cystathionine beta-synthase [Q4QEG9] | |
|---|---|
| Systematic Name | LmjF.17.0250 [Leishmania major] |
| Gene Name | CYSB |
| Molecular Weight | 39205 Da |
| Protein Sequence Size | 359 |
| Function | |
| Charge | -1.5 |
| Isoelectric Point | 6.2 pH |
| Description | Cystathionine beta-synthase (EC 4.2.1.22). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 2.5.1.47; 4.2.1.22 |
| Sequence | >tr|Q4QEG9|Q4QEG9_LEIMA Cystathionine beta-synthase (EC 4.2.1.22) - Leishmania major. ASANPRNQILANALEAVGNTPCIRLNRVPQKHGIRCEVVAKCEFFNPGGSVKDRIGKQMV LDAEKNGTLKPGSVIVEATSGNTGIGLSMAAAIRGYHMVITMPKKMSHEKETTLQSLGAE VIRTETSLPYDHPDSLIGVARRLRDEKGYVLLDQYTNPSNPGAHYEFTGQEIYDQCGSKV DMVVICAGTGGTITGVAKKLKELIPGVIVVGVDPVGSIIADPEHPGEPVMYHVEGIGYDF VPDVCERKYVDRWVKTRDQESFDLALELHREEGLLVGGSSGSAMAGVLEAAKDLRPDQRC VVLMADGIRNYMGKFADVNWMIEHGFRQGEVTRPTYDTLKKELEEVKAKLAKYESGTQ |
| DNA Sequence | >LmjF17.0250 |CYSB||cystathionine beta-synthase|Leishmania major|chr 17|||Manual ATGGCATCCG CAAACCCCCG CAACCAAATC CTGGCCAACG CCCTCGAGGC GGTCGGCAACACCCCTTGCA TCCGCTTGAA CCGCGTCCCA CAGAAGCATG GCATTCGGTG TGAGGTCGTGGCCAAGTGCG AATTCTTCAA CCCTGGCGGC AGCGTGAAGG ATCGCATCGG CAAGCAGATGGTACTCGACG CCGAAAAGAA CGGGACGCTC AAGCCGGGAT CCGTGATTGT GGAGGCTACAAGTGGCAACA CTGGCATCGG TCTCTCCATG GCGGCGGCGA TCCGCGGCTA CCACATGGTGATCACGATGC CGAAGAAGAT GTCGCACGAG AAGGAGACGA CGCTACAGTC CCTTGGCGCCGAGGTGATCC GCACGGAGAC GTCTCTTCCG TACGACCACC CGGACAGCCT CATCGGGGTCGCTCGCCGCC TGCGCGATGA GAAGGGCTAT GTGCTGCTGG ACCAGTACAC GAACCCCAGCAACCCGGGTG CGCACTACGA GTTTACTGGC CAGGAGATCT ACGACCAGTG TGGCAGTAAGGTCGACATGG TAGTCATCTG CGCCGGAACC GGTGGCACGA TCACTGGAGT GGCCAAGAAGCTGAAGGAAC TCATCCCCGG AGTCATCGTT GTCGGTGTAG ACCCGGTCGG CAGCATCATCGCCGATCCCG AACACCCCGG CGAGCCTGTC ATGTACCACG TAGAGGGAAT CGGCTATGACTTTGTGCCAG ATGTGTGCGA GCGTAAGTAC GTCGATCGCT GGGTCAAGAC GCGCGATCAGGAGAGTTTCG ACCTCGCCCT CGAGCTGCAC CGCGAGGAGG GTCTGCTGGT CGGCGGTAGCAGCGGCTCCG CGATGGCCGG CGTGTTGGAG GCAGCCAAGG ACCTTCGCCC GGATCAGCGCTGCGTCGTGC TCATGGCGGA CGGCATCCGC AACTACATGG GGAAGTTTGC CGATGTCAACTGGATGATCG AGCACGGCTT CCGGCAGGGT GAGGTGACCC GCCCCACCTA CGACACGCTGAAGAAGGAGC TGGAGGAGGT CAAGGCGAAG CTGGCCAAGT ACGAGAGCGG CACTCAGTAG |
| Cystathionine beta-synthase Q4QEG9] | |
|---|---|
| Metabolite Information | |
| Molecular Function | catalytic activity; cystathionine beta-synthase activity |
| Biochemical Pathway | cysteine biosynthesis; metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K01697 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | cystathionine-beta-synthase [Homo sapiens] | 48 | 1e-86 | 317 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| thrC threonine synthase | Mycobacterium tuberculosis H37Rv | 26% | 0.0000004 | 49.7 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00700 | Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site | 42-60; | PS00901 | |
| Acylation | N-myristoylation site | 19-24; 85-90; 87-92; 189-194; 191-196; 192-197; 217-222; 274-279; 278-283; 279-284; 282-287; 287-292; | PS00008 | |
| Glycosylation | N-glycosylation site | 67-70; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 105-108; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 51-54; 169-172; 179-182; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 51-53; 69-71; 339-341; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 142-150; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 124-138; | PS00003 | |
| Cystathionine beta-synthase [Q4QEG9] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1m54F | |
| Percent Identity | 48% | |
| Target Region | 1-330 | |
| Template Region | 45-343 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| LYS | 58 | Sidechain |
| SER | 280 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 104414-01-9 | PROTOPORPHYRIN IX CONTAINING FE | 616.487 | C34 H32 Fe N4 O4 | Cc1c2n3c(c1C=C)C=C4C(=C(C5=[N]4[Fe]36[N]7=C(C=C8N6C(=C5)C(=C8C)CCC(=O)O)C(=C(C7=C2)C=C)C)CCC(=O)O)C | 1m54 |
| 54-47-7 | PYRIDOXAL-5'-PHOSPHATE | 247.142 | C8 H10 N O6 P | O=P(O)(O)OCc1cnc(c(O)c1C=O)C | 1m54 |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1825494/Q4QEG9.pdb 2.0 327 = residues | | = | *| Ramachandran plot: 86.8% core 11.0% allow 1.8% gener 0.4% = disall | | = | *| All Ramachandrans: 17 labelled residues (out of 325) = | +| Chi1-chi2 plots: 4 labelled residues (out of 181) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 7 | *| Bond len/angle: 6.0 Morris et al class: 1 = 1 3 | | = | | G-factors Dihedrals: -0.08 Covalent: -0.21 Overall: = -0.12 | | = | | M/c bond lengths: 98.9% within limits 1.1% highlighted = | | M/c bond angles: 92.8% within limits 7.2% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |