| Diphosphomevalonate decarboxylase, putative [Q4QE40] | |
|---|---|
| Systematic Name | LmjF.18.0020 [Leishmania major] |
| Gene Name | LMJF_18_0020 |
| Molecular Weight | 42417 Da |
| Protein Sequence Size | 383 |
| Function | |
| Charge | -1 |
| Isoelectric Point | 6.1648 pH |
| Description | Diphosphomevalonate decarboxylase, putative (EC 4.1.1.33). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 4.1.1.33 |
| Sequence | >tr|Q4QE40|Q4QE40_LEIMA Diphosphomevalonate decarboxylase, putative (EC 4.1.1.33) - Leishmania major. SAPIRVTVEAPINIAFIKYWGKREGGETLILPTNDSFSITLSTKPFRSKTSVELRSDASE DELWLNGKKSNIQETPRIQSVLSCIRDNCPDNTKNLKAYIVSENNFPTAAGMASSASGYC ALAAALVKAYGATVDVSMLSRLGSGSACRSVYGGFVIWHKGEKPDGTDCIATQFLDEKYW PEVQVMCAVLKGEKKDVSSTSGMQQSLKTSSMMRERIESIVPARMSAVKEAIQQRDFNQF AAIAMADSDDLQEICRTTKPPIQYATDDSYAMIRLIRAFNAKKGYNVMAYTFDAGANCFM FTLKQDLPEVVVMLRAHFPTSWDKLLFHDADLLEKCKAYQLPASFEGLIDYPKKSFEMLL QSPMGQGIVYLDDAESLIPPHA |
| DNA Sequence | >LmjF18.0020 |||diphosphomevalonate decarboxylase, putative|Leishmania major|chr 18|||Manual ATGTCGGCGC CGATTCGCGT GACGGTCGAG GCACCCATCA ACATTGCATT CATCAAGTACTGGGGAAAGC GTGAGGGAGG TGAGACGCTG ATCTTACCCA CCAATGACTC CTTTAGTATAACACTCTCCA CAAAACCGTT CCGCTCCAAG ACCTCGGTCG AACTCCGTAG CGACGCCAGCGAAGATGAAC TGTGGCTGAA TGGGAAGAAG AGCAACATTC AAGAGACACC GCGCATCCAGTCAGTTCTCT CGTGTATCCG CGATAACTGC CCAGACAACA CGAAGAATCT GAAGGCGTACATCGTCTCCG AGAACAACTT CCCGACAGCG GCCGGCATGG CCTCCTCTGC CAGCGGCTACTGCGCGCTCG CCGCGGCGCT CGTGAAGGCA TACGGAGCCA CCGTGGACGT GTCGATGCTGTCGCGGCTGG GCTCCGGCAG CGCGTGCCGC AGCGTCTACG GGGGCTTTGT CATTTGGCACAAGGGGGAGA AGCCCGATGG CACGGACTGC ATCGCGACAC AGTTTCTGGA TGAGAAGTACTGGCCAGAGG TGCAGGTGAT GTGCGCCGTT CTCAAGGGCG AGAAGAAGGA TGTGTCGAGCACATCTGGCA TGCAGCAGTC GCTCAAGACG TCCTCCATGA TGCGAGAGCG CATTGAATCCATCGTGCCGG CACGCATGAG CGCCGTCAAG GAGGCCATTC AGCAGCGCGA CTTCAACCAGTTTGCTGCGA TCGCTATGGC TGACTCTGAT GACCTGCAGG AGATTTGCCG CACAACCAAGCCCCCCATCC AGTACGCCAC GGACGACAGC TACGCCATGA TCCGCCTCAT CCGCGCCTTCAACGCCAAGA AGGGCTACAA TGTAATGGCC TACACCTTCG ACGCCGGCGC GAATTGCTTCATGTTCACCC TCAAGCAGGA TCTGCCGGAG GTGGTGGTGA TGCTGCGTGC GCACTTTCCCACCTCCTGGG ACAAGCTGCT TTTTCACGAT GCAGACCTCT TGGAGAAGTG CAAGGCGTACCAGCTGCCCG CATCGTTCGA GGGTCTCATC GATTATCCGA AAAAGTCGTT TGAGATGCTTCTACAGTCTC CCATGGGTCA AGGCATCGTG TACCTCGACG ACGCGGAGTC CCTTATTCCGCCGCACGCCT AG |
| Diphosphomevalonate decarboxylase, putative Q4QE40] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; diphosphomevalonate decarboxylase activity; kinase activity |
| Biochemical Pathway | isoprenoid biosynthesis; phosphorylation |
| Regulatory Pathway | |
| KEGG Pathways | K01597 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | diphosphomevalonate decarboxylase [Homo sapiens] | 43 | 2e-68 | 256 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YNR043w mevalonate pyrophosphate decarboxylase | Saccharomyces cerevisiae | 39% | 4e-60 | 226 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| Acylation | N-myristoylation site | 112-117; 144-149; 203-208; | PS00008 | |
| Amidation | Amidation site | 21-24; 67-70; | PS00009 | |
| Glycosylation | N-glycosylation site | 35-38; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 51-54; 60-63; 321-324; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 43-45; 76-78; 207-209; 258-260; 303-305; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 364-378; | PS00003 | |
| Diphosphomevalonate decarboxylase, putative [Q4QE40] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2hkeB | |
| Percent Identity | 67% | |
| Target Region | 1-383 | |
| Template Region | 4-376 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| THR | 201 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 14808-79-8 | SULFATE ION | 96.063 | O4 S | [O-]S([O-])(=O)=O | 2hke |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/4466840/Q4QE40.pdb 2.0 383 = residues | | = | | Ramachandran plot: 95.3% core 4.7% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 3 labelled residues (out of 381) = | +| Chi1-chi2 plots: 5 labelled residues (out of 221) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | | Residue properties: Max.deviation: 2.5 Bad contacts: = 4 | +| Bond len/angle: 4.8 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | | G-factors Dihedrals: 0.09 Covalent: -0.08 Overall: = 0.03 | | = | | M/c bond lengths: 99.2% within limits 0.8% highlighted = | | M/c bond angles: 95.0% within limits 5.0% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |