| Protein kinase, putative [Q4QE15] | |
|---|---|
| Systematic Name | LmjF.18.0270 [Leishmania major] |
| Gene Name | GSK3 |
| Molecular Weight | 40712 Da |
| Protein Sequence Size | 355 |
| Function | |
| Charge | 7 |
| Isoelectric Point | 7.6 pH |
| Description | Protein kinase, putative (Glycogen synthase kinase, putative) (EC 2.7.1.37). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 2.7.1.37 |
| Sequence | >tr|Q4QE15|Q4QE15_LEIMA Protein kinase, putative (Glycogen synthase kinase, putative) (EC 2.7.1.37) - Leishmania major. SLNAAAAADERSRKEMDRFQVERMAGQGTFGTVQLGKEKSTGMSVAIKKVIQDPRFRNRE LQIMQDLAVLHHPNIVQLQSYFYTLGERDRRDIYLNVVMEYVPDTLHRCCRNYYRRQVAP PPILIKVFLFQLIRSIGCLHLPSVNVCHRDIKPHNVLVNEADGTLKLCDFGSAKKLSPSE PNVAYICSRYYRAPELIFGNQHYTTAVDIWSVGCIFAEMMLGEPIFRGDNSAGQLHEIVR VLGCPSREVLRKLNPSHTDVDLYNSKGIPWSNVFSDHSLKDAKEAYDLLSALLQYLPEER MKPYEALCHPYFDELHDPATKLPNNKDLPEDLFRFLPNEIEVMSEAQKAKLVRK |
| DNA Sequence | >LmjF18.0270 |||protein kinase, putative|Leishmania major|chr 18|||Manual ATGTCGCTCA ACGCTGCCGC TGCCGCGGAT GAGCGAAGTC GCAAGGAGAT GGACCGGTTCCAGGTGGAGC GCATGGCTGG GCAGGGTACA TTCGGCACAG TGCAACTAGG CAAGGAGAAGTCGACGGGCA TGAGCGTGGC GATCAAGAAG GTTATCCAGG ACCCGCGCTT CCGCAACCGCGAGCTGCAGA TCATGCAGGA CCTCGCCGTG CTGCACCACC CCAACATTGT CCAGCTCCAGAGCTACTTCT ACACCCTGGG TGAGCGCGAC CGCCGCGATA TCTACCTCAA TGTCGTGATGGAGTACGTGC CGGATACGTT GCACCGCTGC TGCCGCAACT ACTACCGCCG TCAAGTGGCGCCACCGCCGA TCCTGATCAA GGTCTTCCTT TTTCAGCTGA TCCGAAGTAT CGGGTGCTTGCACCTGCCCT CCGTAAACGT GTGCCACCGC GACATCAAGC CGCACAACGT GCTCGTCAACGAGGCGGACG GCACACTCAA GCTGTGCGAT TTTGGCAGTG CGAAGAAACT CTCGCCGTCGGAGCCGAACG TGGCATACAT CTGCTCTCGT TACTACCGCG CCCCTGAGCT CATCTTCGGTAACCAGCACT ACACGACCGC AGTCGACATC TGGTCGGTGG GGTGTATCTT CGCTGAGATGATGCTTGGCG AGCCCATCTT CCGCGGCGAC AACAGCGCCG GCCAGCTGCA CGAAATTGTGCGCGTGCTCG GCTGCCCTTC GCGCGAGGTG CTGCGTAAGC TGAATCCGTC GCACACGGACGTGGATCTAT ACAATAGCAA GGGCATCCCG TGGAGCAACG TGTTCTCTGA TCATTCGCTGAAGGACGCCA AGGAGGCGTA CGATCTTCTT AGTGCCCTGC TGCAGTACTT GCCGGAGGAGCGCATGAAGC CTTACGAGGC ACTGTGCCAC CCTTACTTCG ACGAGCTTCA CGACCCCGCCACGAAGCTGC CGAATAACAA GGATCTCCCT GAAGACCTCT TCCGCTTCCT GCCGAACGAGATCGAGGTCA TGAGCGAAGC GCAGAAGGCC AAGCTAGTTC GCAAGTA |
| Protein kinase, putative Q4QE15] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity |
| Biochemical Pathway | protein amino acid phosphorylation |
| Regulatory Pathway | |
| KEGG Pathways | K03083 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | glycogen synthase kinase 3 alpha [Homo sapiens] | 49 | 7e-86 | 314 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YDL108w cyclin-dependent serthr protein kinase | Saccharomyces cerevisiae | 32% | 5e-37 | 149 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00016 | Cell attachment sequence | 228-230; | PS00016 | |
| PDOC00100 | Protein kinases signatures and profile | 147-159; | PS00108 | |
| PDOC00100 | Protein kinases signatures and profile | 40.923 | 20-313 | PS50011 |
| Acylation | N-myristoylation site | 29-34; 43-48; 214-219; 268-273; | PS00008 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 175-178; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 13-16; 85-88; 178-181; 206-209; 257-260; 259-262; 279-282; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 13-15; 165-167; 173-175; 279-281; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 280-294; | PS00003 | |
| Protein kinase, putative [Q4QE15] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2o5kA | |
| Percent Identity | 49% | |
| Target Region | 1-355 | |
| Template Region | 35-350 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 151 | Sidechain |
| LYS | 153 | Sidechain |
| ASN | 156 | Sidechain |
| SER | 189 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 2-(2,4-DICHLORO-PHENYL)-7-HYDROXY-1H-BENZOIMIDAZOLE- 4-CARBOXYLIC ACID [2-(4-METHANESULFONYLAMINO- PHENYL)-ETHYL]-AMIDE | 519.4 | C23 H20 Cl2 N4 O4 S | O=S(=O)(Nc1ccc(cc1)CCNC(=O)c2ccc(O)c3nc(nc23)c4ccc(Cl)cc4Cl)C | 2o5k | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1533015/Q4QE15.pdb 2.0 355 = residues | | = | *| Ramachandran plot: 83.8% core 13.0% allow 2.5% gener 0.6% = disall | | = | *| All Ramachandrans: 23 labelled residues (out of 353) = | +| Chi1-chi2 plots: 1 labelled residues (out of 229) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 5.3 Bad contacts: = 11 | *| Bond len/angle: 8.0 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: -0.03 Covalent: -0.20 Overall: = -0.09 | | = | | M/c bond lengths: 98.5% within limits 1.5% highlighted = | *| M/c bond angles: 93.6% within limits 6.4% highlighted 2 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |