Aconitase, putative [Q4QDZ1] | |
---|---|
Systematic Name | LmjF.18.0510 [Leishmania major] |
Gene Name | LMJF_18_0510 |
Molecular Weight | 97492 Da |
Protein Sequence Size | 896 |
Function | |
Charge | 1 |
Isoelectric Point | 6.6285 pH |
Description | Aconitase, putative (EC 4.2.1.3). |
Subcellular Location | N.A.[Predict] |
E. C. Number | 4.2.1.3 |
Sequence | >tr|Q4QDZ1|Q4QDZ1_LEIMA Aconitase, putative (EC 4.2.1.3) - Leishmania major. FRTGVQLAKKGVSPNPFNAKFLASLQVDGGSAKYYKINEISAKYNNLPFSIRVLLESAVR NCDEFDVTSKTVESIFDWKDNCTKGIEIPFKPARVVLQDFTGVPCIVDLAAMRDAMKRLG GDSLRINPQVPVDLVVDHSVQVDCAGVQDAVVQNQSIEMQRNRERFEFLKWGSRAFDNLL IVPPGSGIVHQVNLEYLARVVFNADGMLYPDSVVGTDSHTTMVNGLGVVGWGVGGIEAEA GMLGQSLSLVLPQVVGYKFTGKLQEGCTATDLVLTVVRNLRKLGVVGKFVEFYGPGVDAL SVADRATLANMAPEYGATTGYFPIDNETIEYLKNTNRSAEHVARIESYVKAVGLFRTGNE QIEYSQHLELDLSTVAPCVAGPKRPQDNVPLTDVSRDFKACMSAKSGFKGFGIPEGEHNK KVKYTVNGQEATMEHGSVVIAAITSCTNTSNPTVLIAAGLLAQKALEKGLRVPPGIKTSL SPGSHVVTKYLENAGLQKSLEALGFHTTGYGCMTCIGNSGDIAPEVSKCITDNNFVAAAV LSGNRNFESRIHPLTAANYLASPPLVVAFALAGRANIDFAKEPIANGVYLRDIWPSNEEI VAVVNKYVTPDLFKEVYSNITTMNKQWNELQVENGEFYKWDPKSLYIHSPPYFDDMTLDP PGVKSIENAACLAIFGDSITTDHISPAGNIAKDSPAAKFLMERGVERKDFNTYGSRRGND EVMVRGTFANTRLGNRLVGDGQTGPYTLYHPTGEKMFIFDAAMNYKAAGVPTVILAGKEY GSGSSRDWAAKGPFLQGVKAVIAESFERIHRSNLVGMGVIPLQFKEGENAASLGLTGKEC FSMNFAGELRPRQDIVVKCDNGKTFTTTLRIDTEVEVKYVENGGILNYVLRTKIQ |
DNA Sequence | >LmjF18.0510 |||aconitase, putative|Leishmania major|chr 18|||Manual ATGTTCCGCA CTGGCGTGCA GCTGGCCAAG AAGGGCGTTA GCCCAAACCC CTTCAATGCCAAGTTCCTGG CGTCTCTGCA GGTCGACGGC GGCAGCGCCA AGTACTACAA GATCAACGAGATCAGCGCAA AGTACAACAA CCTCCCCTTC TCCATCCGTG TTCTGCTAGA GTCCGCGGTGCGCAACTGCG ATGAGTTCGA TGTGACCTCG AAGACAGTAG AGAGCATCTT TGACTGGAAGGACAACTGCA CGAAGGGAAT CGAGATTCCG TTCAAGCCAG CTCGCGTGGT GCTGCAGGACTTCACCGGCG TACCGTGCAT CGTGGATCTA GCGGCCATGC GTGACGCGAT GAAGCGCCTTGGCGGTGACT CGCTCCGCAT CAACCCACAA GTTCCCGTCG ACCTCGTAGT GGACCACTCCGTACAGGTGG ACTGCGCTGG CGTGCAGGAC GCAGTGGTGC AAAACCAGAG CATTGAGATGCAACGCAACC GTGAGCGCTT CGAGTTCCTC AAGTGGGGCT CGAGGGCGTT CGACAACCTTCTGATTGTCC CGCCTGGCTC GGGCATTGTG CATCAGGTGA ACCTTGAGTA CCTCGCTCGCGTCGTGTTTA ACGCGGACGG GATGCTATAC CCGGACTCTG TTGTCGGAAC CGACTCGCACACGACGATGG TCAATGGCCT GGGCGTTGTG GGCTGGGGTG TTGGTGGCAT CGAAGCCGAAGCCGGCATGC TGGGCCAGTC CCTCTCCCTG GTGCTGCCGC AGGTCGTTGG CTACAAGTTCACTGGCAAGC TGCAGGAGGG CTGCACGGCA ACCGATCTCG TGCTTACTGT CGTGAGGAACCTTCGCAAGC TCGGTGTGGT GGGCAAGTTT GTCGAGTTCT ACGGCCCCGG TGTCGACGCGCTCTCAGTCG CTGACCGTGC CACGTTGGCC AACATGGCTC CCGAGTACGG CGCCACTACTGGCTACTTCC CCATCGACAA CGAGACGATC GAATACCTCA AGAACACGAA CCGCTCTGCCGAGCACGTGG CCCGCATTGA GAGCTACGTC AAGGCTGTCG GGCTCTTCCG AACCGGCAACGAGCAGATCG AGTACTCGCA GCACCTGGAG CTTGACCTCT CCACAGTGGC GCCGTGCGTTGCCGGCCCGA AGCGCCCTCA AGACAACGTG CCCCTGACGG ATGTGTCGAG GGACTTCAAGGCCTGCATGT CGGCCAAGTC TGGCTTCAAG GGCTTTGGCA TCCCGGAGGG GGAGCACAATAAGAAGGTCA AGTACACCGT CAACGGCCAG GAGGCGACGA TGGAGCACGG CAGCGTCGTGATCGCGGCTA TCACCTCCTG CACGAACACC TCGAACCCCA CCGTGCTCAT CGCGGCGGGTTTGTTGGCAC AGAAGGCCCT GGAAAAGGGC TTGAGGGTGC CGCCGGGCAT TAAGACATCTCTCTCGCCGG GCTCGCACGT GGTGACCAAG TACCTCGAGA ACGCCGGCCT GCAGAAGAGCCTCGAGGCCC TCGGCTTCCA CACGACAGGT TACGGCTGCA TGACGTGCAT CGGCAACTCGGGTGATATCG CGCCGGAGGT GTCCAAGTGC ATCACAGACA ACAATTTCGT TGCCGCAGCGGTGCTCTCCG GCAACCGCAA CTTTGAGTCT CGCATTCACC CGCTGACGGC CGCCAACTACTTGGCATCGC CGCCGCTCGT CGTCGCCTTC GCGCTCGCCG GACGCGCGAA TATCGACTTCGCCAAGGAGC CGATCGCGAA CGGCGTGTAC CTGCGCGACA TCTGGCCAAG CAACGAGGAAATTGTGGCGG TGGTGAACAA GTACGTGACG CCGGACCTGT TCAAGGAGGT GTACTCCAACATCACGACCA TGAACAAGCA ATGGAACGAG CTTCAGGTGG AGAATGGCGA GTTCTACAAGTGGGACCCCA AGTCGTTATA CATTCACAGC CCGCCGTACT TCGACGACAT GACCCTCGACCCACCCGGTG TGAAGAGCAT CGAGAACGCC GCCTGCCTTG CCATCTTCGG TGACTCCATCACGACGGACC ACATCTCGCC GGCCGGCAAC ATAGCCAAGG ACTCACCGGC AGCGAAGTTTCTCATGGAGC GTGGGGTGGA GCGGAAAGAC TTCAACACGT ACGGCTCGCG CCGCGGAAACGACGAGGTGA TGGTGCGCGG CACCTTCGCC AACACGCGTC TCGGCAACCG CCTTGTCGGCGATGGTCAGA CGGGCCCGTA TACGTTGTAC CACCCGACCG GCGAGAAGAT GTTCATCTTCGACGCGGCCA TGAATTACAA GGCGGCCGGC GTGCCGACGG TCATTCTGGC TGGTAAGGAGTACGGCAGCG GCTCGTCGCG CGATTGGGCG GCCAAGGGCC CGTTCCTGCA GGGTGTGAAGGCCGTCATCG CGGAGAGCTT CGAGCGCATT CACCGCTCGA ACCTGGTCGG CATGGGCGTCATTCCGCTGC AGTTCAAGGA AGGCGAGAAT GCCGCCTCCC TTGGCCTGAC CGGCAAGGAGTGCTTCTCGA TGAACTTCGC CGGGGAGCTG CGCCCGCGCC AGGACATCGT CGTGAAGTGCGACAACGGCA AGACCTTCAC GACGACGCTG CGCATTGATA CCGAGGTGGA GGTGAAGTACGTTGAAAACG GTGGCATCCT CAACTATGTG CTGCGCACCA AGATCCAGTA A |
Aconitase, putative Q4QDZ1] | |
---|---|
Metabolite Information | |
Molecular Function | |
Biochemical Pathway | |
Regulatory Pathway | |
KEGG Pathways | K01680 |
Orthologs | ||||
Homologs | GI | Percent Identity | Evalue | Score |
Homo sapiens | aconitase 1 [Homo sapiens] | 57 | 0 | 1019 |
DEG Information | ||||
DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
acn aconitate hydratase | Mycobacterium tuberculosis H37Rv | 49% | 0 | 856 |
Post Translational Modification | ||||
PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
PDOC00423 | Aconitase family signatures | 439-455; | PS00450 | |
PDOC00423 | Aconitase family signatures | 505-518; | PS01244 | |
Acylation | N-myristoylation site | 5-10; 147-152; 235-240; 245-250; 267-272; 317-322; 354-359; 413-418; 429-434; 476-481; 496-501; 512-517; 715-720; 727-732; 782-787; 884-889; | PS00008 | |
Glycosylation | N-glycosylation site | 82-85; 155-158; 327-330; 337-340; 449-452; 620-623; | PS00001 | |
Phosphorylation | Casein kinase II phosphorylation site | 75-78; 269-272; 302-305; 358-361; 597-600; 785-788; 837-840; 874-877; | PS00006 | |
Phosphorylation | Protein kinase C phosphorylation site | 32-34; 42-44; 51-53; 69-71; 124-126; 261-263; 336-338; 404-406; 716-718; 785-787; 837-839; 869-871; | PS00005 | |
Phosphorylation | Tyrosine kinase phosphorylation site | 357-365; | PS00007 | |
Sulfation | Tyrosine sulfation site | 358-372; 873-887; | PS00003 |
Aconitase, putative [Q4QDZ1] | ||
---|---|---|
Model Information | ||
Template PDB ID | 2b3yB | |
Percent Identity | 57% | |
Target Region | 1-896 | |
Template Region | 2-888 |
Domain Information | ||
---|---|---|
Domains | Start | End |
Active Site Information | ||
---|---|---|
Residue | Active Site Number | Functional Part |
ASP | 138 | Sidechain |
HIS | 139 | Sidechain |
HIS | 191 | Sidechain |
ASP | 218 | Sidechain |
HIS | 220 | Sidechain |
GLU | 315 | Sidechain |
SER | 785 | Sidechain |
Co-Factor | |
---|---|
Metal | Description |
Fe, S | Binds 1 4Fe-4S cluster per subunit |
Ligands | |||||
---|---|---|---|---|---|
CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
71-50-1 | ACETATE ION | 59.044 | C2 H3 O2 | [O-]C(=O)C | 2b3y |
992-98-3 | FORMIC ACID | 46.025 | C H2 O2 | O=CO | 2b3y |
8013-25-0 | GLYCEROL | 92.094 | C3 H8 O3 | OCC(O)CO | 2b3y |
IRON/SULFUR CLUSTER | 351.64 | Fe4 S4 | [S]12[Fe]3[S]4[Fe]1[S]5[Fe]2[S]3[Fe]45 | 2b3y |
Mutational Information | ||
---|---|---|
Residue | Feature | Description |
|
|
Modeled Protein | Template Structure |
|
+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/79266/Q4QDZ1.pdb 2.0 896 = residues | | = | *| Ramachandran plot: 93.6% core 5.7% allow 0.4% gener 0.3% = disall | | = | +| All Ramachandrans: 15 labelled residues (out of 894) = | +| Chi1-chi2 plots: 4 labelled residues (out of 491) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 6.8 Bad contacts: = 7 | *| Bond len/angle: 5.8 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.08 Covalent: -0.11 Overall: = 0.01 | | = | | M/c bond lengths: 99.5% within limits 0.5% highlighted = | | M/c bond angles: 93.5% within limits 6.5% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |