| Citrate synthase, putative [Q4QDX3] | |
|---|---|
| Systematic Name | LmjF.18.0680 [Leishmania major] |
| Gene Name | LmjF18.0680 |
| Molecular Weight | 52329 Da |
| Protein Sequence Size | 470 |
| Function | |
| Charge | 5 |
| Isoelectric Point | 7.1929 pH |
| Description | Citrate synthase, putative (EC 2.3.3.1). |
| Subcellular Location | cytoplasm[Predict] |
| E. C. Number | 2.3.3.1 |
| Sequence | >tr|Q4QDX3|Q4QDX3_LEIMA Citrate synthase, putative (EC 2.3.3.1) - Leishmania major. RAVRCSLIRGVAGLRMASSALDEMKEQMLRRWKEDQKKIDDLRKKHGHEKLCDATIDAVY GGMRGITGLVYEPSLLDPAEGIRFRGLTILECQEMLPKAPGGKEPLPEAMFWLLMTGEVP TEEQVRGLNAELHRRADPEAIAAAQKAIAALPRNAHPMTAFSVGVLALQSYSKFAAAYAA GKSNKKTYWEYALEDSLDMLARTPTVAAMIYNRETKGQVELAAPSNSDLDWAANFAKMMG YQDEEFWECMRLYLSVHADHEGGNVSAHTTTLVASALSDPYLAFSAGLNGLAGPLHGLAN QEVLNYLLSMQERVKADGVNMHDEAALEKALSNYTWELLNSGQVVPGYGHAVLRKVDPRY TCQRNFCLRHKFDDDLFKLVNTIYSIMPGILKEHGKTKNPYPNVDAHSGVLLQHYGLTEQ NYYTVLFGLSRQMGVLAGVVWDRLQGRPLERPKSITTEMLAKKYLCNSL |
| DNA Sequence | >LmjF18.0680 |||citrate synthase, putative|Leishmania major|chr 18|||Manual ATGCGCGCTG TTCGCTGCTC CCTAATTCGC GGCGTCGCCG GTCTCCGCAT GGCGTCATCGGCATTGGATG AGATGAAGGA GCAGATGCTG AGGCGCTGGA AAGAGGACCA GAAGAAGATCGACGACCTCA GGAAGAAGCA CGGCCATGAG AAGCTGTGCG ACGCGACCAT CGATGCGGTGTACGGCGGCA TGCGCGGCAT CACCGGCCTC GTGTACGAGC CATCACTGCT GGACCCCGCAGAGGGCATCC GCTTCCGCGG CCTCACGATC CTGGAGTGCC AGGAGATGCT GCCCAAAGCGCCGGGCGGCA AGGAGCCGCT GCCGGAGGCG ATGTTCTGGC TACTGATGAC CGGCGAGGTGCCGACGGAGG AGCAGGTAAG GGGCCTGAAC GCGGAGCTGC ACCGTCGCGC CGACCCCGAGGCGATTGCCG CGGCGCAGAA GGCGATCGCG GCGCTGCCGA GGAACGCGCA CCCGATGACGGCATTCAGTG TGGGCGTGCT TGCGCTGCAG AGCTACTCGA AGTTTGCTGC GGCTTACGCGGCGGGCAAGT CGAACAAGAA GACGTACTGG GAGTACGCGC TGGAGGACTC GCTGGACATGCTGGCGCGCA CGCCGACGGT GGCGGCGATG ATCTACAACC GCGAGACCAA GGGCCAGGTGGAGTTGGCGG CACCGAGCAA CAGCGACCTG GACTGGGCGG CGAACTTTGC GAAAATGATGGGCTACCAGG ACGAGGAGTT CTGGGAGTGC ATGCGTCTGT ACCTGTCTGT CCACGCCGACCACGAGGGTG GAAACGTGTC GGCACACACA ACGACACTGG TTGCGTCAGC ACTGAGCGACCCCTACCTCG CCTTCAGCGC TGGCCTGAAC GGTCTTGCTG GCCCGCTGCA TGGGCTGGCGAACCAGGAGG TGCTGAATTA CTTGCTCAGC ATGCAGGAGC GTGTGAAGGC GGATGGCGTGAACATGCATG ATGAGGCAGC GCTCGAGAAG GCGCTGAGCA ACTACACGTG GGAGTTGCTCAACTCCGGCC AGGTGGTGCC CGGCTACGGC CACGCGGTGC TGCGCAAGGT GGACCCGCGCTACACCTGCC AGCGCAACTT CTGCCTGCGC CACAAATTTG ATGACGACCT GTTCAAGCTGGTCAACACCA TCTACTCGAT CATGCCCGGC ATCCTGAAGG AGCACGGTAA GACCAAGAACCCCTACCCCA ACGTCGACGC GCACTCCGGC GTGCTACTGC AGCACTACGG GCTGACGGAGCAGAACTACT ACACGGTGCT GTTCGGCCTG TCGCGCCAGA TGGGCGTCTT GGCCGGCGTCGTCTGGGACC GCCTGCAGGG CCGCCCGCTC GAGCGCCCGA AGTCGATCAC GACGGAGATGCTCGCAAAGA AATACCTGTG CAACTCCTTG TGA |
| Citrate synthase, putative Q4QDX3] | |
|---|---|
| Metabolite Information | |
| Molecular Function | citrate (Si)-synthase activity; transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer |
| Biochemical Pathway | main pathways of carbohydrate metabolism; tricarboxylic acid cycle |
| Regulatory Pathway | |
| KEGG Pathways | K01647 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | citrate synthase precursor, isoform a [Homo sapiens] | 55 | 1e-137 | 484 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| HP0775 penta-phosphate guanosine-3'-pyrophosphohydrolase (spoT) | Helicobacter pylori | 23% | 0.51 | 30 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 177-184; | PS00017 | |
| PDOC00422 | Citrate synthase signature | 348-360; | PS00480 | |
| PDOC00015 | The rule PS00015 has been deleted from the PROSITE database and replaced by the following motif: PS50079 (Bipartite nuclear localization signal profile | 31-47; | PS00015 | |
| Acylation | N-myristoylation site | 14-19; 62-67; 263-268; 264-269; 435-440; | PS00008 | |
| Glycosylation | N-glycosylation site | 265-268; 334-337; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 20-23; 75-78; 89-92; 188-191; 226-229; 228-231; 310-313; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 184-186; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 355-361; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 185-199; 235-249; | PS00003 | |
| Citrate synthase, putative [Q4QDX3] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2ctsA | |
| Percent Identity | 56% | |
| Target Region | 20-470 | |
| Template Region | 1-437 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| SER | 248 | Sidechain |
| HIS | 278 | Sidechain |
| HIS | 332 | Sidechain |
| ASP | 387 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 5949-29-1 | CITRIC ACID | 192.124 | C6 H8 O7 | O=C(O)CC(O)(C(=O)O)CC(=O)O | 2cts |
| 31416-98-5 | COENZYME A | 767.534 | C21 H36 N7 O16 P3 S | O=C(NCCS)CCNC(=O)C(O)C(C)(C)COP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3OP(=O)(O)O | 2cts |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1388340/Q4QDX3.pdb 2.0 450 = residues | | = | *| Ramachandran plot: 94.6% core 4.6% allow 0.5% gener 0.3% = disall | | = | +| All Ramachandrans: 9 labelled residues (out of 444) = | +| Chi1-chi2 plots: 4 labelled residues (out of 259) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 6.6 Bad contacts: = 6 | *| Bond len/angle: 7.2 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.08 Covalent: -0.16 Overall: = -0.01 | | = | | M/c bond lengths: 98.9% within limits 1.1% highlighted = | | M/c bond angles: 93.4% within limits 6.6% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |