| Elongation factor TU, putative [Q4QDW8] | |
|---|---|
| Systematic Name | LmjF.18.0740 [Leishmania major] |
| Gene Name | LMJF_18_0740 |
| Molecular Weight | 51536 Da |
| Protein Sequence Size | 466 |
| Function | |
| Charge | 6.5 |
| Isoelectric Point | 7.3 pH |
| Description | Elongation factor TU, putative. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QDW8|Q4QDW8_LEIMA Elongation factor TU, putative - Leishmania major FRSGVHRLAPKAKEAFVRGKPHLIIGTIGHVDHGKTTLTSAITTVLAKRGQAQALDYFAI DKSPEEKSRKITINATHVEYESEKRHYGHIDCPGHMDFVKNMITGAAQMDGGIIVVAATD GVMPQTREHLLICSQIGLPALVGFINKVDMTDEDTCDLVDMEVREQLEKYKFPAEETPIV RGSALKAVEGDAKYEENILELVRKCDEWIPDPPRNTDKPFLMAIEHVYEIGKDKKSVIVT GRVDQGVLKLNTDAELAGFSAKKSTVRVTGIEMYHKTLSECMPGDSVGVSIVGTGDTTSL SKDNVERGMVMAATGSTNLYNKVKAQVYVLTKDEGGRHTGFSPHYRPQLFFHCADVTADM SFPEAEKHREELNKKFGRGPEEDKKKEAEMKEFESKLVCMPGDNRELILTLAYPMPIEKG LKFTIREGKITVGWGAVVETMGLDTKVSIEGKRIGAKPVTGKKKK |
| DNA Sequence | >LmjF18.0740 |||elongation factor TU, putative|Leishmania major|chr 18|||Manual ATGTTTCGCT CGGGTGTGCA TCGCCTTGCC CCCAAGGCGA AGGAGGCGTT TGTTCGCGGTAAGCCGCATC TCATCATCGG CACCATCGGT CACGTCGACC ACGGCAAGAC GACGCTGACGTCGGCCATCA CGACGGTTCT GGCGAAGCGC GGTCAGGCAC AGGCGCTGGA CTACTTCGCCATCGACAAGT CCCCGGAGGA GAAGAGCCGC AAAATCACCA TCAATGCCAC GCACGTCGAGTACGAGTCGG AGAAACGCCA CTACGGACAC ATCGACTGTC CCGGCCACAT GGACTTTGTGAAAAACATGA TCACCGGAGC TGCGCAGATG GACGGTGGCA TCATTGTGGT GGCGGCCACCGACGGCGTCA TGCCGCAGAC ACGCGAACAC CTCCTGATCT GCTCGCAAAT TGGCCTTCCGGCGCTCGTAG GGTTCATCAA CAAAGTGGAC ATGACGGACG AGGACACGTG TGACCTGGTGGACATGGAGG TGCGCGAGCA GCTGGAGAAA TACAAGTTTC CGGCGGAGGA GACACCAATCGTGCGCGGCT CGGCCCTCAA GGCCGTCGAG GGCGACGCGA AGTACGAGGA GAACATCCTCGAACTGGTGC GGAAGTGCGA CGAGTGGATC CCCGACCCGC CGCGCAACAC AGACAAGCCTTTCCTTATGG CCATCGAGCA CGTTTACGAG ATCGGCAAGG ACAAGAAGAG CGTCATCGTGACCGGCCGCG TCGATCAGGG CGTGCTGAAG CTCAACACAG ACGCCGAGCT GGCCGGCTTCAGCGCCAAGA AGTCGACGGT GAGGGTGACG GGCATTGAAA TGTACCACAA GACGCTGAGCGAGTGCATGC CCGGTGACTC CGTCGGCGTC AGCATTGTCG GCACCGGCGA CACGACCAGTCTGTCCAAGG ACAACGTGGA GCGCGGCATG GTAATGGCGG CGACGGGTAG CACGAACCTGTACAACAAGG TGAAGGCGCA GGTGTACGTG CTGACGAAGG ATGAGGGCGG CCGCCACACTGGCTTCAGCC CCCACTACCG CCCGCAGCTC TTCTTCCATT GCGCTGACGT GACAGCGGACATGAGCTTCC CGGAGGCGGA GAAGCACCGC GAGGAGCTCA ACAAGAAATT CGGCCGCGGCCCCGAGGAGG ACAAGAAGAA AGAGGCGGAG ATGAAGGAGT TCGAGAGCAA GCTCGTCTGCATGCCGGGCG ATAACCGCGA GCTGATCCTG ACGCTGGCGT ACCCGATGCC CATTGAAAAGGGTCTGAAGT TCACCATCCG TGAGGGCAAG ATCACCGTCG GCTGGGGCGC CGTGGTGGAGACGATGGGCC TCGACACGAA GGTGAGCATT GAAGGCAAGC GCATCGGCGC AAAGCCGGTGACTGGCAAGA AGAAGAAGTA G |
| Elongation factor TU, putative Q4QDW8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | GTP binding; translation elongation factor activity |
| Biochemical Pathway | protein biosynthesis; translational elongation |
| Regulatory Pathway | |
| KEGG Pathways | K02358 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | Tu translation elongation factor, mitochondrial [Homo sapiens] | 43 | 3e-97 | 352 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| tufA translational elongation factor TU | Staphylococcus aureus | 49% | 1e-117 | 414 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 30-37; | PS00017 | |
| Acylation | N-myristoylation site | 51-56; 294-299; 309-314; 336-341; 421-426; | PS00008 | |
| Amidation | Amidation site | 451-454; 461-464; | PS00009 | |
| Glycosylation | N-glycosylation site | 75-78; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 70-73; 263-266; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 64-67; 77-80; 152-155; 253-256; 270-273; 278-281; 332-335; 362-365; 425-428; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 69-71; 83-85; 217-219; 241-243; 261-263; 266-268; 425-427; 461-463; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 188-202; | PS00003 | |
| Elongation factor TU, putative [Q4QDW8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2fx3A | |
| Percent Identity | 49% | |
| Target Region | 19-444 | |
| Template Region | 7-384 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 15 | Sidechain |
| HIS | 78 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 146-91-8 | GUANOSINE-5'-DIPHOSPHATE | 443.201 | C10 H15 N5 O11 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c2N=C(N)NC1=O)C(O)C3O | 2fx3 |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 2fx3 |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1528562/Q4QDW8.pdb 2.0 425 = residues | | = | *| Ramachandran plot: 89.1% core 8.7% allow 0.8% gener 1.4% = disall | | = | *| All Ramachandrans: 14 labelled residues (out of 423) = | +| Chi1-chi2 plots: 5 labelled residues (out of 250) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 7 | *| Bond len/angle: 6.8 Morris et al class: 1 = 1 3 | | = | | G-factors Dihedrals: 0.00 Covalent: -0.19 Overall: = -0.07 | | = | | M/c bond lengths: 98.8% within limits 1.2% highlighted = | | M/c bond angles: 93.5% within limits 6.5% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |