| Pyruvate dehydrogenase E1 component alpha subunit, putative [Q4QDQ1] | |
|---|---|
| Systematic Name | LmjF.18.1380 [Leishmania major] |
| Gene Name | LMJF_18_1380 |
| Molecular Weight | 42874 Da |
| Protein Sequence Size | 378 |
| Function | |
| Charge | 7 |
| Isoelectric Point | 7.8 pH |
| Description | Pyruvate dehydrogenase E1 component alpha subunit, putative (EC 1.2.4.1). |
| Subcellular Location | Mitochondrion[Predict] |
| E. C. Number | 1.2.4.1 |
| Sequence | >tr|Q4QDQ1|Q4QDQ1_LEIMA Pyruvate dehydrogenase E1 component alpha subunit, putative (EC 1.2.4.1) - Leishmania major. FKCATRCLLDTKTVPLKPQRPFKLHTAGRTDMAPLPTQAVYDAEQLKQSLALMFRIRRME SLCDQSYKLKKIRGFCHLYIGQEAIPAGMENVLTFEDPIITGYRDHGWYISRGGKPEDVF AEMFGRQGGCSKGKGGSMHMYRVDNGFYGGNGIVGAQVSIGAGLAWRFAMENRDSPKHVA VTFYGDGAANQGQIYESMNIAALQRLPVIFAVENNHFGMGTSAARGSYQAEFYRRGDYIP GIKVDGMDVLAVQEGTRYARDYCMSGKGPIVMELDCYRYMGHSMSDPDNQYRTKSDIQHV KQERDCIRKMREFMATEGIMTEDEMSKMEKDVKKEVDQDLQKAQKQPMTKLDELFTDIYV GEQYEHRTCQGTVYHKP |
| DNA Sequence | >LmjF18.1380 |||pyruvate dehydrogenase E1 component alpha subunit, putative|Leishmania major|chr 18|||Manual ATGTTCAAGT GCGCGACTCG CTGCCTTCTG GACACCAAGA CGGTGCCGCT GAAGCCGCAGCGCCCCTTCA AGCTGCACAC GGCTGGCCGC ACCGACATGG CACCCCTGCC GACGCAGGCGGTATACGACG CAGAGCAGCT GAAGCAGAGC CTTGCCCTGA TGTTCCGCAT CCGCCGCATGGAGTCTCTGT GCGATCAGTC CTACAAGCTA AAGAAGATTC GCGGCTTCTG CCACCTCTACATCGGGCAGG AGGCCATCCC GGCTGGCATG GAGAACGTTC TGACCTTCGA GGACCCCATTATCACTGGCT ACCGTGATCA CGGCTGGTAC ATTTCTCGCG GTGGCAAGCC CGAGGATGTCTTCGCTGAAA TGTTCGGTCG TCAGGGCGGC TGCAGCAAGG GCAAGGGTGG CTCGATGCACATGTACAGGG TGGATAACGG CTTCTACGGC GGTAATGGTA TCGTCGGTGC GCAGGTCTCCATCGGCGCTG GCCTTGCCTG GCGCTTCGCG ATGGAGAACC GCGACAGCCC GAAGCACGTCGCGGTCACCT TCTACGGCGA CGGTGCTGCC AACCAGGGCC AGATCTACGA GTCCATGAACATTGCCGCTC TGCAGCGCCT CCCCGTCATC TTCGCCGTGG AGAACAACCA CTTCGGTATGGGCACTAGCG CCGCTCGCGG CTCCTATCAG GCGGAGTTTT ACCGCCGTGG TGACTACATCCCTGGTATCA AGGTCGATGG CATGGACGTG CTCGCGGTGC AGGAGGGCAC CCGCTACGCACGCGACTACT GCATGTCGGG CAAAGGCCCG ATTGTGATGG AGCTGGACTG CTACCGCTACATGGGCCATA GCATGTCAGA CCCCGACAAC CAGTACCGCA CCAAGAGCGA CATCCAGCACGTGAAGCAGG AGCGTGACTG CATTCGCAAG ATGCGCGAGT TCATGGCAAC GGAGGGCATCATGACCGAGG ACGAGATGAG CAAGATGGAG AAAGACGTAA AGAAGGAGGT GGACCAGGACCTGCAGAAGG CCCAGAAACA GCCTATGACA AAGCTGGATG AGCTCTTCAC GGACATCTACGTGGGTGAGC AGTACGAGCA CCGCACTTGC CAGGGCACTG TGTACCACAA GCCCTAA |
| Pyruvate dehydrogenase E1 component alpha subunit, putative Q4QDQ1] | |
|---|---|
| Metabolite Information | |
| Molecular Function | oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor |
| Biochemical Pathway | metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K00161 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | pyruvate dehydrogenase (lipoamide) alpha 1 [Homo sapiens] | 46 | 5e-79 | 291 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| pdhA pyruvate dehydrogenase (E1 alpha subunit) | Bacillus subtilis | 30% | 4e-32 | 132 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00016 | Cell attachment sequence | 236-238; | PS00016 | |
| PDOC00015 | The rule PS00015 has been deleted from the PROSITE database and replaced by the following motif: PS50079 (Bipartite nuclear localization signal profile | 58-74; | PS00015 | |
| Acylation | N-myristoylation site | 130-135; 153-158; 156-161; 162-167; 219-224; 221-226; 227-232; 256-261; | PS00008 | |
| Phosphorylation | Casein kinase II phosphorylation site | 62-65; 95-98; 284-287; 286-289; 294-297; 322-325; 327-330; 350-353; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 67-69; 176-178; 266-268; | PS00005 | |
| Pyruvate dehydrogenase E1 component alpha subunit, putative [Q4QDQ1] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1ni4C | |
| Percent Identity | 46% | |
| Target Region | 1-381 | |
| Template Region | 1-362 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| HIS | 276 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Thiamine pyrophosphate | |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 24203-36-9 | POTASSIUM ION | 39.098 | K | [K+] | 1ni4 |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 1ni4 |
| 154-87-0 | THIAMINE DIPHOSPHATE | 425.314 | C12 H19 N4 O7 P2 S | O=P(O)(O)OP(=O)(O)OCCc1sc[n+](c1C)Cc2c(nc(nc2)C)N | 1ni4 |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2945696/Q4QDQ1.pdb 2.0 378 = residues | | = | *| Ramachandran plot: 92.0% core 6.2% allow 1.2% gener 0.6% = disall | | = | +| All Ramachandrans: 5 labelled residues (out of 376) = | +| Chi1-chi2 plots: 4 labelled residues (out of 237) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 6 | *| Bond len/angle: 7.6 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.15 Covalent: -0.12 Overall: = 0.05 | | = | | M/c bond lengths: 99.5% within limits 0.5% highlighted = | | M/c bond angles: 94.7% within limits 5.3% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |