| Methionine aminopeptidase, putative [Q4QDG7] | |
|---|---|
| Systematic Name | LmjF.19.0550 [Leishmania major] |
| Gene Name | LMJF_19_0550 |
| Molecular Weight | 44867 Da |
| Protein Sequence Size | 401 |
| Function | |
| Charge | -1 |
| Isoelectric Point | 6.3905 pH |
| Description | Methionine aminopeptidase, putative (Metallo-peptidase, clan mg, family m24) (EC 3.4.11.18). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 3.4.11.18 |
| Sequence | >tr|Q4QDG7|Q4QDG7_LEIMA Methionine aminopeptidase, putative (Metallo-peptidase, clan mg, family m24) (EC 3.4.11.18) - Leishmania major. PCEGCGVGEAGLQCPTCKKLSLPPSFFCTQDCFRAHWGTHKLKHTETKNLPATIPTMTEV DERLFNFTGPLRPGKITPRRAVPKEIARPDYAERNDGVSELEEKDRGSLRVVAHSLKNLH EDYNNAELRRSSDILKIKRVNALSREVLDIACAAVKPGVTTDEIDRIVHEATIERGMYPS PLNYYNFPKSVCTSVNEIICHGIPDNRPLEEGDIVNIDVSCYLDGFHGDLNETVFVGRPD EESVKIVHTAYACMMAGIGVVKPDELYRYIGDAIEARAKKSGCSVVRSYTGHGIGKFFHT APNVCHYKDNKSPGLIKPGHVFTIEPMINLGTWQDVTWPDNWTSATKDGKRTAQFEHTMV CTPEAVELLTDWKDGIPFYQKQLREWGIPIPAEDPSEIKI |
| DNA Sequence | >LmjF19.0550 |||methionine aminopeptidase, putative|Leishmania major|chr 19|||Manual ATGCCCTGCG AAGGCTGCGG CGTCGGCGAG GCCGGCCTGC AGTGCCCCAC TTGCAAGAAACTGAGCTTGC CGCCGAGCTT CTTCTGCACG CAGGACTGCT TCCGGGCGCA CTGGGGAACGCACAAGCTGA AGCACACTGA GACGAAGAAC CTGCCCGCCA CGATCCCCAC CATGACGGAGGTAGACGAGC GGCTCTTCAA CTTCACGGGG CCACTCCGTC CTGGAAAAAT CACGCCACGCCGTGCTGTGC CAAAGGAGAT AGCGCGGCCC GACTACGCGG AGCGAAACGA CGGCGTTTCCGAGCTGGAGG AGAAAGACCG AGGCAGCCTC CGCGTCGTTG CACACAGCCT CAAGAACTTGCACGAAGACT ACAACAACGC CGAGCTGCGC CGGAGCTCCG ACATCCTCAA GATCAAGCGTGTGAACGCGC TCTCGCGTGA AGTGCTGGAC ATCGCCTGCG CGGCGGTGAA GCCGGGCGTCACAACCGACG AAATCGACCG CATCGTCCAC GAGGCGACGA TCGAGCGTGG CATGTACCCATCGCCGCTGA ATTACTACAA CTTCCCCAAG TCCGTCTGCA CCAGCGTGAA TGAGATCATCTGCCACGGCA TTCCAGACAA CCGCCCACTC GAGGAGGGCG ACATCGTGAA CATCGACGTCTCTTGCTACC TCGACGGCTT CCACGGCGAC TTAAACGAAA CGGTGTTTGT CGGCAGGCCAGACGAGGAAA GTGTGAAGAT TGTGCACACG GCGTATGCCT GCATGATGGC GGGCATCGGCGTTGTGAAGC CGGATGAACT CTACCGCTAC ATCGGTGACG CCATCGAGGC ACGGGCGAAGAAGTCGGGCT GCTCTGTCGT GCGCAGCTAC ACCGGACATG GTATCGGCAA GTTCTTCCACACGGCACCGA ACGTCTGCCA CTACAAGGAC AACAAAAGTC CCGGCCTCAT CAAGCCCGGGCACGTCTTCA CGATTGAGCC GATGATCAAC CTTGGCACGT GGCAGGATGT CACATGGCCTGATAACTGGA CGAGTGCCAC GAAGGATGGC AAGCGCACCG CGCAGTTTGA GCACACGATGGTGTGCACAC CAGAAGCGGT AGAGCTTCTG ACGGACTGGA AGGACGGCAT ACCCTTTTATCAGAAGCAGC TGAGGGAATG GGGCATTCCG ATTCCCGCCG AAGACCCCAG CGAAATCAAAATCTGA |
| Methionine aminopeptidase, putative Q4QDG7] | |
|---|---|
| Metabolite Information | |
| Molecular Function | metalloexopeptidase activity; methionyl aminopeptidase activity |
| Biochemical Pathway | proteolysis |
| Regulatory Pathway | |
| KEGG Pathways | K01265 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | methionyl aminopeptidase 1 [Homo sapiens] | 61 | 4e-87 | 318 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| map Methionine aminopeptidase (EC 3.4.11.18) | Escherichia coli MG1655 | 42% | 2e-53 | 204 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00575 | Methionine aminopeptidase signatures | 290-308; | PS00680 | |
| Acylation | N-myristoylation site | 5-10; 7-12; 203-208; | PS00008 | |
| Amidation | Amidation site | 349-352; | PS00009 | |
| Glycosylation | N-glycosylation site | 67-70; 232-235; 342-345; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 19-22; 130-133; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 57-60; 59-62; 100-103; 161-164; 195-198; 333-336; 338-341; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 17-19; 40-42; 78-80; 109-111; 116-118; 244-246; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 263-270; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 117-131; | PS00003 | |
| Methionine aminopeptidase, putative [Q4QDG7] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2nq7A | |
| Percent Identity | 54% | |
| Target Region | 1-383 | |
| Template Region | 90-304 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASN | 304 | Sidechain |
| GLU | 326 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 22541-53-3 | COBALT (II) ION | 58.933 | Co | [Co+2] | 2nq7 |
| 8013-25-0 | GLYCEROL | 92.094 | C3 H8 O3 | OCC(O)CO | 2nq7 |
| 3-[(2,2-DIMETHYLPROPANOYL)AMINO]-N-1,3-THIAZOL- 2-YLPYRIDINE-2-CARBOXAMIDE | 304.367 | C14 H16 N4 O2 S | O=C(c1ncccc1NC(=O)C(C)(C)C)Nc2nccs2 | 2nq7 | |
| 24203-36-9 | POTASSIUM ION | 39.098 | K | [K+] | 2nq7 |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/898765/Q4QDG7.pdb 2.0 383 = residues | | = | *| Ramachandran plot: 93.3% core 5.5% allow 0.6% gener 0.6% = disall | | = | +| All Ramachandrans: 7 labelled residues (out of 381) = | | Chi1-chi2 plots: 0 labelled residues (out of 218) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 9 | *| Bond len/angle: 6.2 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | | G-factors Dihedrals: 0.06 Covalent: -0.16 Overall: = -0.02 | | = | | M/c bond lengths: 98.6% within limits 1.4% highlighted = | *| M/c bond angles: 94.6% within limits 5.4% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |