| Glycosomal malate dehydrogenase [Q4QDF0] | |
|---|---|
| Systematic Name | LmjF.19.0710 [Leishmania major] |
| Gene Name | gMDH |
| Molecular Weight | 33631 Da |
| Protein Sequence Size | 322 |
| Function | |
| Charge | 8 |
| Isoelectric Point | 9.2 pH |
| Description | Glycosomal malate dehydrogenase (EC 1.1.1.37). |
| Subcellular Location | glycosome[Predict] |
| E. C. Number | 1.1.1.37 |
| Sequence | >tr|Q4QDF0|Q4QDF0_LEIMA Glycosomal malate dehydrogenase (EC 1.1.1.37) - Leishmania major. VNVCVVGAAGGIGQSLSLLLVRQLPYGSTLSLFDVVGAAGVAADLSHVDNAGVQVKFAEG KIGHKRDPALAELAKGVDVFVMVAGVPRKPGMTRDDLFKINAGIILDLVLTCASSSPKAV FCIVTNPVNSTVAIAAEALKSLGVYDRNRLLGVSLLDGLRATCFINEARKPLVVSQVPVV GGHSDTTIVPLFYQLPGPLPEQATLDKIVKRVQVAGTEVVKAKAGRGSATLSMAEAGARF ALKVVEGLTGTGNPLVYAYVDTDGQHETTFLAIPVVLGMNGIEKRLPIGPLHSTEETLLK AALPVIKKNIVKGSEFARSHL |
| DNA Sequence | >LmjF19.0710 |||glycosomal malate dehydrogenase|Leishmania major|chr 19|||Manual ATGGTGAACG TGTGCGTTGT TGGTGCTGCC GGCGGCATTG GCCAGTCGCT GTCGCTGCTGTTGGTGCGCC AGCTGCCGTA CGGGAGCACG TTGTCGCTGT TCGACGTTGT GGGCGCTGCAGGCGTCGCAG CAGACCTGTC GCATGTGGAC AACGCCGGTG TGCAGGTGAA GTTTGCGGAGGGCAAGATCG GCCATAAGCG CGACCCTGCG CTGGCAGAGC TTGCGAAGGG CGTGGATGTGTTTGTAATGG TGGCTGGCGT TCCACGCAAG CCGGGCATGA CGCGCGACGA CCTTTTCAAAATCAACGCCG GAATCATCCT GGACCTTGTG CTGACGTGCG CGTCGTCGAG TCCAAAGGCGGTGTTCTGCA TTGTGACGAA CCCTGTGAAC AGCACGGTCG CGATCGCGGC AGAGGCGCTGAAGAGCCTTG GCGTATACGA CCGAAACCGG CTGCTTGGCG TGTCGCTGCT GGACGGGCTGCGCGCGACGT GCTTCATCAA CGAGGCGCGC AAGCCCTTAG TCGTGTCGCA GGTACCAGTTGTTGGCGGGC ACAGCGACAC AACGATTGTG CCGTTGTTCT ACCAGCTACC GGGGCCGTTGCCGGAGCAGG CGACGCTGGA CAAGATCGTG AAGCGCGTGC AGGTCGCAGG CACAGAAGTGGTGAAGGCGA AGGCCGGGCG CGGGTCTGCG ACGCTGTCGA TGGCGGAGGC TGGCGCGCGGTTCGCGTTGA AGGTTGTGGA GGGTCTGACC GGCACGGGTA ACCCGCTGGT GTACGCATATGTAGACACAG ACGGGCAGCA CGAGACGACG TTCCTCGCGA TCCCTGTGGT GCTTGGCATGAATGGAATCG AGAAGCGCCT GCCGATTGGT CCGCTGCACT CGACGGAGGA AACGCTGCTGAAGGCGGCAC TGCCGGTGAT CAAGAAGAAT ATCGTGAAGG GCAGCGAGTT CGCGCGCTCACACCTGTAG |
| Glycosomal malate dehydrogenase Q4QDF0] | |
|---|---|
| Metabolite Information | |
| Molecular Function | L-malate dehydrogenase activity; oxidoreductase activity |
| Biochemical Pathway | malate metabolism; tricarboxylic acid cycle; tricarboxylic acid cycle intermediate metabolism |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | mitochondrial malate dehydrogenase precursor [Homo sapiens] | 44 | 3e-65 | 245 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| mdh malate dehydrogenase | Haemophilus influenzae | 48% | 1e-67 | 250 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00299 | Microbodies C-terminal targeting signal | 320-322; | PS00342 | |
| Acylation | N-myristoylation site | 8-13; 12-17; 14-19; 28-33; 159-164; 238-243; 248-253; 265-270; 314-319; | PS00008 | |
| Glycosylation | N-glycosylation site | 130-133; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 32-35; 47-50; 94-97; 155-158; 233-236; 294-297; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 117-119; | PS00005 | |
| Glycosomal malate dehydrogenase [Q4QDF0] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2pwzG | |
| Percent Identity | 49% | |
| Target Region | 1-324 | |
| Template Region | 1-312 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 158 | Sidechain |
| ARG | 161 | Sidechain |
| HIS | 184 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/344164/Q4QDF0.pdb 2.0 322 = residues | | = | +| Ramachandran plot: 94.5% core 5.2% allow 0.4% gener 0.0% = disall | | = | +| All Ramachandrans: 4 labelled residues (out of 320) = | *| Chi1-chi2 plots: 5 labelled residues (out of 156) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 3.2 Bad contacts: = 4 | *| Bond len/angle: 5.9 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.07 Covalent: -0.11 Overall: = 0.00 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | | M/c bond angles: 94.3% within limits 5.7% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |