| Mitogen-activated protein kinase [Q4QD66] | |
|---|---|
| Systematic Name | LmjF.19.1440 [Leishmania major] |
| Gene Name | MPK4 |
| Molecular Weight | 41481 Da |
| Protein Sequence Size | 363 |
| Function | |
| Charge | 0.5 |
| Isoelectric Point | 6.5 pH |
| Description | Mitogen-activated protein kinase (Map kinase). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QD66|Q4QD66_LEIMA Mitogen-activated protein kinase (Map kinase) - Leishmania major. AQLVPLAELPSGKKIYSVRGQRFEVDREYDLVKVVGFGACGTVCSAVANGSGERVAIKRL SRVFGDLREGKRILREMEIMTSLKHNNLIRLHHFMRPQSKETFEDIYLVMDLYDTDLNRI IRSRQKLTDEHLQYFMIQAFRGLHYLHSAKVMHRDLKPSNLLVNADCALAICDFGLARDD QVMSSSDLTQYVVTRWYRPPEVLGMGSNQYTSAVDVWSLGLIFAELMVGRALLPGTDYIG QLVMIVNLLGSPSIDDMEFLSSEAKAFILSQPHRPALSFRDLFSMATEEATDLLSKLLVF HPARRLTAKQVMEHPYFSKYRDAAEEADAPDPFVWNHSHIETKEQLREDLWRVVEAHSQL NE |
| DNA Sequence | >LmjF19.1440 |||mitogen-activated protein kinase|Leishmania major|chr 19|||Manual ATGGCTCAAC TCGTCCCTTT AGCTGAACTG CCCAGCGGCA AAAAAATATA TAGTGTCCGGGGGCAGCGTT TCGAAGTGGA CAGGGAATAT GATCTGGTCA AGGTTGTTGG ATTTGGTGCGTGTGGCACTG TTTGTTCAGC GGTCGCGAAC GGGTCGGGTG AGCGAGTGGC GATCAAGCGGTTGTCGCGTG TTTTTGGTGA TCTTCGTGAA GGGAAACGAA TTTTGCGGGA GATGGAGATAATGACGTCGC TGAAGCACAA TAATCTGATT CGCCTCCACC ACTTCATGCG GCCGCAGTCAAAGGAGACGT TTGAGGACAT TTACTTGGTG ATGGATCTTT ATGACACAGA TTTAAATCGTATTATACGAA GTCGGCAGAA ACTCACTGAT GAGCATCTGC AGTATTTTAT GATTCAAGCGTTCCGCGGAT TGCATTACCT TCACTCTGCC AAGGTGATGC ATCGCGATCT GAAGCCGAGCAACTTGCTTG TAAATGCGGA CTGCGCGCTA GCAATCTGCG ATTTTGGGCT GGCTCGTGATGATCAAGTCA TGAGCTCGTC AGATCTCACA CAGTACGTCG TAACACGGTG GTACAGACCCCCTGAGGTAC TCGGGATGGG ATCCAATCAG TACACGAGCG CGGTAGATGT CTGGAGCCTTGGTCTGATCT TTGCGGAGCT AATGGTGGGG CGTGCCTTGC TTCCGGGAAC AGATTATATTGGACAGCTAG TGATGATTGT CAACCTATTA GGGTCCCCGT CCATAGATGA CATGGAGTTTCTGAGCTCAG AAGCAAAGGC GTTTATTCTC TCTCAGCCGC ATCGGCCGGC TCTCTCCTTCAGAGATCTTT TTTCAATGGC TACAGAAGAG GCCACTGACC TTCTGTCGAA GCTGCTAGTTTTCCATCCAG CGAGACGATT AACTGCGAAG CAAGTGATGG AACATCCATA TTTTTCGAAGTACAGAGATG CCGCAGAAGA AGCTGACGCT CCTGATCCGT TTGTGTGGAA TCATAGCCATATAGAAACCA AGGAGCAACT CCGTGAGGAT TTGTGGCGGG TTGTTGAAGC CCATTCACAATTGAACGAAT AG |
| Mitogen-activated protein kinase Q4QD66] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; protein kinase activity; protein serine/threonine kinase activity; protein-tyrosine kinase activity |
| Biochemical Pathway | protein amino acid phosphorylation |
| Regulatory Pathway | |
| KEGG Pathways | K08293 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | mitogen-activated protein kinase 1 [Homo sapiens] | 43 | 7e-69 | 258 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YDL108w cyclin-dependent serthr protein kinase | Saccharomyces cerevisiae | 34% | 1e-40 | 161 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00595 | Formate--tetrahydrofolate ligase signatures | 50-53; 337-340; | PS00722 | |
| PDOC01049 | MAP kinase signature | 65-168; | PS01351 | |
| PDOC00100 | Protein kinases signatures and profile | 152-164; | PS00108 | |
| PDOC00100 | Protein kinases signatures and profile | 44.471 | 30-318 | PS50011 |
| Acylation | N-myristoylation site | 39-44; 42-47; 205-210; 221-226; | PS00008 | |
| Amidation | Amidation site | 12-15; 70-73; | PS00009 | |
| Glycosylation | N-glycosylation site | 50-53; 337-340; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 59-62; 305-308; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 103-106; 185-188; 213-216; 254-257; 279-282; 339-342; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 12-14; 18-20; 83-85; 149-151; 279-281; 308-310; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 23-30; 101-108; 127-135; 310-317; | PS00007 | |
| Mitogen-activated protein kinase [Q4QD66] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2ozaB | |
| Percent Identity | 45% | |
| Target Region | 12-365 | |
| Template Region | 6-340 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 145 | Sidechain |
| LYS | 147 | Sidechain |
| ASN | 150 | Sidechain |
| THR | 184 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/464625/Q4QD66.pdb 2.0 352 = residues | | = | *| Ramachandran plot: 87.8% core 10.6% allow 1.2% gener 0.3% = disall | | = | *| All Ramachandrans: 13 labelled residues (out of 350) = | +| Chi1-chi2 plots: 1 labelled residues (out of 227) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 5 | *| Bond len/angle: 6.9 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.00 Covalent: -0.19 Overall: = -0.07 | | = | | M/c bond lengths: 99.5% within limits 0.5% highlighted = | | M/c bond angles: 93.7% within limits 6.3% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |