| Phosphoglycerate kinase C, glycosomal [Q4QD34] | |
|---|---|
| Systematic Name | LmjF.20.0100 [Leishmania major] |
| Gene Name | PGKC |
| Molecular Weight | 51542 Da |
| Protein Sequence Size | 479 |
| Function | |
| Charge | 13 |
| Isoelectric Point | 9.1158 pH |
| Description | Phosphoglycerate kinase C, glycosomal (EC 2.7.2.3). |
| Subcellular Location | cytoplasm; glycosome[Predict] |
| E. C. Number | 2.7.2.3 |
| Sequence | >tr|Q4QD34|Q4QD34_LEIMA Phosphoglycerate kinase C, glycosomal (EC 2.7.2.3) - Leishmania major. SLVLKKSIDDATVRDKKVLIRVDFNVPVKNGKITNDFRIRSALPTIQKVLKEGGSCILMS HLGRPKGARMSDPSPEKGVRGYEEAATLRPVAARIAELLGQKVEFAPDCLDAAAYASKLK NGDVLLLENVRFYAEEGSKKEEERDAMAKVLASYADLYVSDAFGTAHRDSATMTGIPKVL GAGYAGYLMEKEINYFSRVLNNPPRPLVAIVGGAKVSDKIELLDNMLSRINYLVIGGAMA YTFQKAQGRKIGISMCEEDKLDLAKSLLKKAQERGVQVFLPVDHVCNKEFKAADSPLVTE SVDVPDGYMALDIGPRTIHMYEEVIGRCKSAIWNGPMGVFEMPCYSKGTFAVAKAMGTGT QKNGLLSIIGGGDSASAAELSGEAKNMSHVSTGGGASLELLEGKTLPGVAILTDKDVKER GASCRFAFGVGSPSREACPLRCGHIFGGASIVREIVKLVVALLIGIFIGRRMSTKLIR |
| DNA Sequence | >LmjF20.0100 |PGKC||phosphoglycerate kinase C, glycosomal|Leishmania major|chr 20|||Manual ATGTCTCTCG TGCTGAAGAA GAGCATCGAT GACGCCACCG TCAGGGACAA GAAGGTGCTCATCCGCGTGG ACTTCAACGT CCCCGTGAAG AACGGTAAGA TCACGAATGA CTTCCGCATTCGCTCCGCCC TTCCGACGAT CCAAAAGGTG CTGAAGGAGG GCGGCTCCTG CATCCTGATGAGCCACCTTG GTCGGCCGAA GGGTGCGAGA ATGAGTGACC CAAGTCCGGA GAAGGGTGTGCGCGGGTACG AGGAGGCCGC CACGCTGCGC CCGGTTGCTG CACGCATCGC GGAGCTGCTGGGGCAGAAGG TGGAGTTTGC ACCGGACTGC CTGGACGCTG CGGCGTACGC GTCGAAGCTGAAGAACGGCG ACGTGCTGTT GCTGGAGAAC GTGCGTTTCT ACGCGGAGGA GGGCAGCAAGAAGGAGGAGG AGCGCGACGC GATGGCGAAG GTACTGGCGT CGTACGCGGA CCTGTATGTCAGTGACGCCT TTGGCACTGC GCACCGCGAC AGCGCGACGA TGACGGGCAT CCCCAAGGTGCTGGGTGCGG GCTACGCCGG GTACCTGATG GAGAAGGAGA TCAACTACTT CTCGCGAGTGCTGAACAACC CGCCGCGGCC GCTGGTTGCG ATTGTCGGCG GTGCGAAGGT CAGCGACAAGATCGAGCTGC TTGACAACAT GCTGAGCCGC ATCAACTACC TTGTGATTGG TGGCGCGATGGCGTACACGT TCCAGAAGGC GCAGGGCCGC AAGATCGGCA TCTCGATGTG CGAGGAGGATAAGCTGGACC TTGCCAAGTC GCTGCTGAAG AAGGCGCAAG AGCGCGGTGT GCAGGTGTTTCTGCCAGTTG ACCACGTGTG CAACAAGGAA TTTAAGGCCG CGGACTCGCC GCTGGTGACGGAGAGCGTGG ACGTCCCGGA CGGCTACATG GCGCTCGACA TTGGCCCAAG GACGATCCACATGTACGAGG AAGTGATTGG CCGCTGCAAG AGCGCGATCT GGAACGGCCC GATGGGCGTGTTCGAGATGC CGTGCTACTC GAAGGGCACA TTTGCCGTTG CGAAGGCGAT GGGGACTGGAACGCAGAAGA ACGGGCTGCT GAGCATCATC GGCGGTGGTG ACAGCGCGAG CGCTGCGGAGCTGAGCGGCG AGGCGAAGAA CATGTCGCAT GTGTCCACCG GCGGCGGCGC GTCCCTGGAGCTGCTGGAGG GCAAGACGCT GCCTGGCGTC GCCATTCTGA CGGACAAGGA CGTGAAGGAGCGGGGAGCTT CGTGCAGGTT CGCTTTCGGG GTCGGGAGCC CGAGCAGGGA GGCTTGTCCGCTCCGTTGTG GGCATATCTT TGGCGGTGCC TCCATCGTAA GAGAGATAGT TAAGCTCGTCGTCGCGCTGC TGATCGGCAT TTTTATTGGT CGCCGCATGA GCACCAAGCT GATCCGGTAA |
| Phosphoglycerate kinase C, glycosomal Q4QD34] | |
|---|---|
| Metabolite Information | |
| Molecular Function | phosphoglycerate kinase activity |
| Biochemical Pathway | glycolysis |
| Regulatory Pathway | |
| KEGG Pathways | K00927 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | phosphoglycerate kinase 1 [Homo sapiens] | 41 | 2e-85 | 313 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| pgk phosphoglycerate kinase | Bacillus subtilis | 45% | 9e-94 | 338 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00102 | Phosphoglycerate kinase signature | 18-28; | PS00111 | |
| Acylation | N-myristoylation site | 68-73; 182-187; 214-219; 237-242; 253-258; 360-365; 371-376; 372-377; 394-399; 466-471; | PS00008 | |
| Amidation | Amidation site | 248-251; 469-472; | PS00009 | |
| Glycosylation | N-glycosylation site | 387-390; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 471-474; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 8-11; 13-16; 139-142; 154-157; 255-258; 377-380; 414-417; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 13-15; 88-90; 139-141; 218-220; 361-363; 414-416; 424-426; 474-476; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 127-141; | PS00003 | |
| Phosphoglycerate kinase C, glycosomal [Q4QD34] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 16pkA | |
| Percent Identity | 71% | |
| Target Region | 1-420 | |
| Template Region | 5-415 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ARG | 39 | Sidechain |
| LYS | 216 | Sidechain |
| GLY | 373 | Backbone amide |
| GLY | 395 | Backbone amide |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 1,1,5,5-TETRAFLUOROPHOSPHOPENTYLPHOSPHONIC ACID ADENYLATE ESTER | 633.276 | C15 H22 F4 N5 O12 P3 | FC(F)(P(=O)(O)O)CCCC(F)(F)P(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 16pk | |
| 7365-45-9 | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | 238.305 | C8 H18 N2 O4 S | O=S(=O)(O)CCN1CCN(CCO)CC1 | 16pk |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/3011904/Q4QD34.pdb 2.0 416 = residues | | = | *| Ramachandran plot: 96.1% core 3.4% allow 0.3% gener 0.3% = disall | | = | +| All Ramachandrans: 3 labelled residues (out of 414) = | +| Chi1-chi2 plots: 2 labelled residues (out of 233) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 11.7 Bad contacts: = 1 | *| Bond len/angle: 10.7 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.13 Covalent: -0.15 Overall: = 0.03 | | = | | M/c bond lengths: 99.6% within limits 0.4% highlighted = | *| M/c bond angles: 94.3% within limits 5.7% highlighted 3 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |