| Ribonuclease L inhibitor, putative [Q4QCE4] | |
|---|---|
| Systematic Name | LmjF.21.0710 [Leishmania major] |
| Gene Name | ABCE1 |
| Molecular Weight | 73977 Da |
| Protein Sequence Size | 659 |
| Function | |
| Charge | 14 |
| Isoelectric Point | 8.2 pH |
| Description | Ribonuclease L inhibitor, putative (Atp-binding cassette sub-family e, putative). |
| Subcellular Location | cytoplasm[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QCE4|Q4QCE4_LEIMA Ribonuclease L inhibitor, putative (Atp-binding cassette sub-family e, putative) - Leishmania major. PPKKQAEEQSRLTRIAVVNADRCKPRKCNLECYKCCPVVLQGKLCIEVSHQSTISKISEE LCIGCALCVKKCPYDAIRIINLPSNLERDTVHRYGPNSFKLHRLPLPRPGQVLGLVGANG TGKSTALSVLKGRIRPNLGRYMNEPGWDEILKYFRGSEHQAYFQHLLDDKMRVLLKPQYV DKVPKTNNGVVEVLLTKADERSMKDYFMEVLDLKNVADRTLDKLSGGELQRFTIAALCVQ NAQVYMYDEPSSYLDVRQRLTAAQVIRSMLTETNYVIVVEHDLSVVDYMSDFVCVLYGVP GIYGVVTMPYGVREGINIFLDGFVPTENLRFREEGLTFHIADDIEEGVSKRRAMNEYPAM SKNLGSFSLSVDAGTFSDSEIVVLLGENGCGKTTFIRMLAGHVKPDNDVEVPKLSISYKP QKIAPKFQGTVRDLLQTKIYEMYCHPQFQTDVLKPLTIEELLDQEVQNLSGGQLQRVGLC IALGTPANIYLIDEPSAYLDSDQRIIASRVIKRFILNSKRTAFIVEHDFIMATYLADKVI VYDGTPAVNCTARTPCGLLEGMNRFLKSLNITFRRDPTNFRPRINKLDSVKDRDQKASGN YFYMTDMEDPKGKKPAQKNGDDSDNGDGADVKAAPKKQQNQRHDGGGNDGKGKKAHKN |
| DNA Sequence | >LmjF21.0710 |||ribonuclease L inhibitor, putative|Leishmania major|chr 21|||Manual ATGCCACCAA AGAAGCAGGC CGAAGAGCAG TCGCGGCTTA CCCGTATTGC AGTCGTCAATGCGGACCGCT GCAAGCCGCG CAAGTGCAAC CTCGAGTGCT ACAAGTGCTG CCCTGTTGTGCTGCAGGGTA AGCTGTGTAT CGAGGTGAGC CACCAAAGCA CCATCTCCAA GATTTCCGAGGAACTTTGCA TTGGCTGCGC GCTGTGCGTG AAGAAGTGCC CCTACGATGC GATTCGTATCATCAACCTCC CCTCTAACCT AGAGAGGGAC ACTGTGCACC GGTACGGCCC TAACAGCTTCAAGCTGCATC GCCTTCCACT GCCTCGTCCC GGACAGGTGC TTGGCCTTGT TGGTGCGAACGGTACGGGCA AATCGACTGC CCTCTCGGTC CTGAAGGGCC GCATTAGGCC GAACCTCGGAAGATACATGA ATGAGCCTGG GTGGGACGAG ATTCTCAAGT ACTTCCGCGG CTCTGAGCATCAGGCGTACT TCCAGCATCT ACTAGACGAC AAAATGCGCG TTCTTCTGAA GCCGCAGTACGTAGACAAGG TGCCAAAGAC CAACAACGGG GTGGTGGAGG TTCTTCTTAC CAAGGCAGACGAGCGTAGCA TGAAGGATTA CTTCATGGAG GTTCTTGATC TCAAGAACGT GGCGGATCGTACCTTGGACA AGCTGTCGGG TGGTGAGCTG CAGCGCTTCA CCATTGCCGC TCTTTGCGTGCAGAATGCGC AGGTGTACAT GTACGATGAG CCCTCTAGCT ACCTTGACGT GCGCCAGCGTCTTACCGCCG CCCAGGTCAT CCGCTCCATG CTGACTGAGA CCAACTACGT GATCGTCGTAGAGCATGACC TGTCCGTGGT GGACTACATG TCTGATTTCG TCTGTGTGCT GTACGGCGTTCCTGGCATCT ACGGTGTGGT GACAATGCCC TATGGTGTGC GCGAGGGCAT CAACATCTTCCTTGATGGTT TTGTGCCGAC GGAGAACCTG CGGTTCCGTG AGGAGGGCCT CACATTCCACATCGCAGACG ATATTGAAGA GGGCGTCAGC AAGCGCCGCG CTATGAATGA GTACCCAGCCATGAGCAAGA ACCTAGGCTC CTTCAGCCTC TCCGTAGATG CCGGCACCTT CTCCGACTCTGAGATTGTGG TGCTTCTGGG CGAGAATGGT TGCGGGAAGA CCACTTTCAT TCGCATGCTCGCTGGCCACG TGAAGCCGGA CAACGACGTC GAGGTGCCGA AGCTGTCCAT CAGTTACAAGCCCCAGAAGA TCGCGCCAAA GTTCCAGGGC ACGGTGCGTG ACCTTCTCCA GACGAAGATCTACGAAATGT ACTGCCATCC ACAGTTTCAG ACGGATGTTC TGAAGCCGCT CACGATCGAGGAGCTTCTGG ACCAGGAAGT CCAGAATCTC TCCGGTGGTC AGCTCCAGCG TGTCGGTCTGTGCATTGCAC TGGGTACGCC GGCCAACATC TACCTCATCG ATGAGCCCAG TGCGTACCTTGACTCCGATC AGCGTATCAT CGCCTCCCGC GTGATCAAGC GCTTCATCCT GAACAGCAAGCGCACGGCGT TCATTGTGGA GCACGACTTT ATCATGGCCA CATACCTGGC CGACAAAGTTATCGTGTACG ACGGTACACC CGCCGTGAAT TGCACAGCAC GGACGCCGTG CGGCCTCCTTGAAGGCATGA ATAGGTTCCT CAAGAGCCTC AACATTACCT TCCGCCGCGA CCCCACGAACTTCCGACCTC GCATCAACAA GCTGGACTCC GTCAAGGACC GCGATCAGAA GGCTTCGGGCAACTACTTCT ACATGACGGA TATGGAGGAC CCGAAGGGGA AGAAGCCTGC GCAGAAGAACGGAGACGACT CAGACAACGG CGACGGAGCT GACGTGAAGG CGGCGCCCAA GAAGCAACAGAACCAGCGCC ACGACGGTGG CGGCAATGAT GGGAAGGGGA AGAAGGCCCA CAAGAACTAG |
| Ribonuclease L inhibitor, putative Q4QCE4] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; ATPase activity; electron transporter activity; iron ion binding; nucleoside-triphosphatase activity; nucleotide binding; ribonuclease activity |
| Biochemical Pathway | electron transport; mRNA catabolism, exonucleolytic |
| Regulatory Pathway | |
| KEGG Pathways | K06174 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | ATP-binding cassette, sub-family E, member 1 [Homo sapiens] | 62 | 0 | 780 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YDR091c strong similarity to human RNase L inhibitor and M.jannasch | Saccharomyces cerevisiae | 63% | 0 | 776 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00176 | 4Fe-4S ferredoxins, iron-sulfur binding region signature | 63-74; | PS00198 | |
| PDOC00185 | ATP-binding cassette, ABC transporter-type, signature and profile | 470-484; | PS00211 | |
| PDOC00185 | ATP-binding cassette, ABC transporter-type, signature and profile | 13.085; 14.233 | 78-323; 352-570 | PS50893 |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 118-125; 387-394; | PS00017 | |
| Acylation | N-myristoylation site | 65-70; 111-116; 115-120; 118-123; 121-126; 312-317; 390-395; 479-484; 485-490; 558-563; 647-652; | PS00008 | |
| Amidation | Amidation site | 612-615; 652-655; | PS00009 | |
| Glycosylation | N-glycosylation site | 120-123; 469-472; 550-553; 571-574; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 85-88; 197-200; 203-206; 226-229; 253-256; 285-288; 376-379; 378-381; 431-434; 458-461; 590-593; 606-609; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 99-101; 122-124; 203-205; 350-352; 418-420; 431-433; 519-521; 552-554; 573-575; 590-592; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 156-163; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 247-261; 492-506; | PS00003 | |
| Ribonuclease L inhibitor, putative [Q4QCE4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1yqtA | |
| Percent Identity | 47% | |
| Target Region | 90-606 | |
| Template Region | 78-515 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 58-64-0 | ADENOSINE-5'-DIPHOSPHATE | 427.201 | C10 H15 N5 O10 P2 | O=P(O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 1yqt |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 1yqt |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/2249882/Q4QCE4.pdb 2.0 515 = residues | | = | *| Ramachandran plot: 92.4% core 7.0% allow 0.2% gener 0.4% = disall | | = | +| All Ramachandrans: 11 labelled residues (out of 505) = | +| Chi1-chi2 plots: 1 labelled residues (out of 311) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 3 | *| Bond len/angle: 6.1 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.14 Covalent: -0.10 Overall: = 0.05 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | | M/c bond angles: 94.5% within limits 5.5% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |