| Hypoxanthine-guanine phosphoribosyltransferase [Q4QCC3] | |
|---|---|
| Systematic Name | LmjF.21.0845 [Leishmania major] |
| Gene Name | HGPRT |
| Molecular Weight | 23570 Da |
| Protein Sequence Size | 211 |
| Function | |
| Charge | 2.5 |
| Isoelectric Point | 7.9259 pH |
| Description | Hypoxanthine-guanine phosphoribosyltransferase. |
| Subcellular Location | cytoplasm[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QCC3|Q4QCC3_LEIMA Hypoxanthine-guanine phosphoribosyltransferase - Leishmania major. SNSAKSPSGIVGDEGRRNYPMSAHTLVTQEQVWAATAKCAKKIAEDYRSFKLTTGNPLYL LCVLKGSFIFTADLARFLADEGVPVKVEFICASSYGTGVETSGQVRMLLDVRDSVENRHI LIVEDIVDSAITLQYLMRFMLAKKPASLKTVVLLDKPSGRKVEVLVDYPVITIPHAFVIG YGMDYAESYRELRDICVLKKEYYEKPESKV |
| DNA Sequence | >LmjF21.0845 |HGPRT||hypoxanthine-guanine phosphoribosyltransferase|Leishmania major|chr 21|||Manual ATGAGCAACT CCGCCAAGTC GCCCTCCGGC ATCGTCGGCG ATGAGGGGCG GCGCAACTATCCGATGTCAG CCCACACACT CGTCACACAG GAGCAGGTAT GGGCCGCCAC GGCGAAGTGCGCAAAGAAGA TTGCAGAGGA CTACAGGAGT TTTAAGTTGA CGACCGGGAA CCCGCTCTACCTGCTGTGCG TGCTCAAGGG CAGCTTCATC TTCACGGCCG ACCTCGCCCG CTTTCTCGCCGACGAGGGTG TCCCGGTAAA GGTGGAGTTT ATTTGCGCGA GCTCGTACGG CACGGGCGTGGAGACGTCGG GCCAGGTGCG CATGCTCCTC GACGTGCGCG ACTCCGTGGA GAATCGCCATATTCTGATTG TCGAGGACAT CGTCGACAGC GCCATCACGC TGCAGTATCT GATGCGGTTCATGCTCGCCA AGAAGCCGGC CTCGCTCAAG ACGGTGGTGC TGCTGGACAA GCCGTCGGGGCGAAAGGTGG AGGTACTAGT CGACTACCCT GTCATCACGA TCCCGCACGC GTTTGTGATTGGCTACGGTA TGGACTACGC CGAGTCGTAC CGCGAGCTGC GCGATATCTG CGTGCTCAAGAAGGAGTACT ACGAGAAGCC GGAGAGCAAG GTGTAG |
| Hypoxanthine-guanine phosphoribosyltransferase Q4QCC3] | |
|---|---|
| Metabolite Information | |
| Molecular Function | hypoxanthine phosphoribosyltransferase activity |
| Biochemical Pathway | nucleoside metabolism; purine ribonucleoside salvage |
| Regulatory Pathway | |
| KEGG Pathways | K00760 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | hypoxanthine phosphoribosyltransferase 1 [Homo sapiens] | 33 | 9e-19 | 90 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| hprT hypoxanthine-guanine phosphoribosyltransferase | Bacillus subtilis | 40% | 9e-33 | 134 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00299 | Microbodies C-terminal targeting signal | 209-211; | PS00342 | |
| PDOC00096 | Purine/pyrimidine phosphoribosyl transferases signature | 121-133; | PS00103 | |
| Acylation | N-myristoylation site | 99-104; 183-188; | PS00008 | |
| Amidation | Amidation site | 15-18; 159-162; | PS00009 | |
| Phosphorylation | Casein kinase II phosphorylation site | 98-101; 189-192; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 4-6; 37-39; 50-52; 148-150; 159-161; 189-191; | PS00005 | |
| Hypoxanthine-guanine phosphoribosyltransferase [Q4QCC3] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1pzmB | |
| Percent Identity | 90% | |
| Target Region | 20-201 | |
| Template Region | 20-170 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| GLU | 106 | Sidechain |
| ASP | 107 | Sidechain |
| ASP | 110 | Sidechain |
| LYS | 138 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 85-32-5 | GUANOSINE-5'-MONOPHOSPHATE | 363.221 | C10 H14 N5 O8 P | O=C1c2ncn(c2N=C(N)N1)C3OC(C(O)C3O)COP(=O)(O)O | 1pzm |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/4411305/Q4QCC3.pdb 2.0 182 = residues | | = | +| Ramachandran plot: 90.9% core 7.9% allow 1.2% gener 0.0% = disall | | = | +| All Ramachandrans: 3 labelled residues (out of 180) = | +| Chi1-chi2 plots: 1 labelled residues (out of 105) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 6.2 Bad contacts: = 2 | *| Bond len/angle: 12.2 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.07 Covalent: -0.25 Overall: = -0.04 | | = | | M/c bond lengths: 99.2% within limits 0.8% highlighted = | *| M/c bond angles: 93.9% within limits 6.1% highlighted 2 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |