| Thymidine kinase, putative [Q4QC75] | |
|---|---|
| Systematic Name | LmjF.21.1210 [Leishmania major] |
| Gene Name | LMJF_21_1210 |
| Molecular Weight | 31602 Da |
| Protein Sequence Size | 284 |
| Function | |
| Charge | 5 |
| Isoelectric Point | 7.9918 pH |
| Description | Thymidine kinase, putative (EC 2.7.1.21). |
| Subcellular Location | Mitochondrion[Predict] |
| E. C. Number | 2.7.1.21 |
| Sequence | >tr|Q4QC75|Q4QC75_LEIMA Thymidine kinase, putative (EC 2.7.1.21) - Leishmania major. FRGRIELIIGPMFAGKTTELMRRVKREIHARRSCFVIKYSKDTRYDEHNVASHDQLMLRA QAAVSQLTEVRDTWKRFDVLAIDEGQFFSDLVDFCNTAADAGKVVMVSALDGDYRRKPFG QICELVPYCEAVDKLTAVCMMCHEQPACFTRRTVNVEQQELIGGADMYIATCRECYSKQQ LPSIEEMRTQRMAIKEVEKRYLGLSDKRATASPQTPEKPAGGWCTKAGVATLPTMVKEGA ASSGASAGMKSGRGLCEMQAFTTETPKYQRVEPACTASAASSE |
| DNA Sequence | >LmjF21.1210 |||thymidine kinase, putative|Leishmania major|chr 21|||Manual ATGTTCCGCG GTCGTATAGA GCTCATTATC GGCCCGATGT TTGCCGGCAA GACAACGGAGCTGATGCGCC GCGTCAAGCG CGAGATCCAC GCCCGTCGCA GCTGCTTCGT CATCAAGTACTCCAAGGATA CCCGCTACGA TGAGCACAAC GTTGCTTCAC ACGACCAGCT GATGCTGCGGGCGCAGGCGG CCGTCTCGCA GCTGACGGAG GTGCGGGACA CGTGGAAGCG GTTCGACGTGCTGGCGATCG ACGAGGGTCA GTTTTTTTCT GACCTGGTAG ATTTTTGCAA CACTGCCGCAGACGCGGGCA AGGTAGTCAT GGTGTCGGCC CTCGACGGCG ATTACCGGCG CAAGCCGTTTGGGCAGATCT GCGAGCTCGT CCCGTACTGC GAGGCGGTGG ACAAGCTGAC GGCGGTGTGCATGATGTGCC ACGAGCAGCC CGCCTGCTTT ACTCGGCGCA CCGTGAACGT GGAGCAGCAGGAGCTCATTG GTGGCGCAGA CATGTACATC GCGACCTGCC GCGAGTGCTA CTCGAAGCAGCAGTTGCCCT CCATCGAGGA AATGCGGACG CAGCGGATGG CGATCAAGGA GGTGGAGAAGCGGTACCTCG GCTTGTCAGA CAAGAGGGCA ACCGCCAGTC CCCAGACACC CGAGAAACCGGCTGGTGGCT GGTGCACTAA GGCCGGGGTC GCGACGCTCC CCACGATGGT AAAGGAGGGAGCGGCCTCGT CCGGGGCGTC GGCCGGTATG AAGTCGGGTC GAGGCCTGTG CGAGATGCAGGCCTTCACCA CCGAGACGCC CAAGTACCAG CGTGTGGAGC CTGCGTGCAC GGCCTCGGCTGCATCCTCAG AGTGA |
| Thymidine kinase, putative Q4QC75] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; thymidine kinase activity |
| Biochemical Pathway | nucleobase, nucleoside and nucleotide interconversion |
| Regulatory Pathway | |
| KEGG Pathways | K00857 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | thymidine kinase 1, soluble [Homo sapiens] | 50 | 2e-42 | 169 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| tdk thymidine kinase | Mycoplasma genitalium | 34% | 3e-20 | 93.2 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00017 | ATP/GTP-binding site motif A (P-loop) | 11-18; | PS00017 | |
| PDOC00524 | Thymidine kinase cellular-type signature | 164-177; | PS00603 | |
| Acylation | N-myristoylation site | 86-91; 222-227; 240-245; 245-250; 249-254; | PS00008 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 208-211; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 44-47; 98-101; 109-112; 172-175; 184-187; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 74-76; 151-153; 172-174; 190-192; 206-208; 252-254; 266-268; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 39-46; 196-202; | PS00007 | |
| Thymidine kinase, putative [Q4QC75] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2orvB | |
| Percent Identity | 51% | |
| Target Region | 1-179 | |
| Template Region | 18-160 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| P1-(5'-ADENOSYL)P4-(5'-(2'-DEOXY-THYMIDYL))TETRAPHOSPHATE | 807.342 | C20 H25 N7 O20 P4 | CC1=CN(C(=O)NC1=O)C2CC(C(O2)COP(=O)([O-])OP(=O)([O-])OP(=O)([O-])OP(=O)([O-])OCC3C(C(C(O3)n4cnc5c4ncnc5N)O)O)O | 2orv | |
| 23713-49-7 | ZINC ION | 65.409 | Zn | [Zn+2] | 2orv |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/4607940/Q4QC75.pdb 2.0 179 = residues | | = | +| Ramachandran plot: 87.8% core 8.5% allow 3.7% gener 0.0% = disall | | = | *| All Ramachandrans: 7 labelled residues (out of 177) = | *| Chi1-chi2 plots: 5 labelled residues (out of 109) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.2 Bad contacts: = 10 | *| Bond len/angle: 18.5 Morris et al class: 1 = 1 2 | | = | +| G-factors Dihedrals: -0.16 Covalent: -1.27 Overall: = -0.55 | | = | *| M/c bond lengths: 93.8% within limits 6.2% highlighted 3 off = graph | *| M/c bond angles: 86.6% within limits 13.4% highlighted 10 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |