| Ribonucleoside-diphosphate reductase small chain, putative [Q4QBL8] | |
|---|---|
| Systematic Name | LmjF.22.1290 [Leishmania major] |
| Gene Name | LMJF_22_1290 |
| Molecular Weight | 44395 Da |
| Protein Sequence Size | 392 |
| Function | |
| Charge | -15 |
| Isoelectric Point | 4.7491 pH |
| Description | Ribonucleoside-diphosphate reductase small chain, putative. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QBL8|Q4QBL8_LEIMA Ribonucleoside-diphosphate reductase small chain, putative - Leishmania major SSEPKAMDTCAAPAKRARTEEAETEVARPSGEAAGVSNPSTAEDLVTKPVAAGAEVLPAD KVAEGTNAEEEPLQQENPFRYVLFPIQYHDIWRKYKEQESCIWTVEEIDLGNDMKDWATL NDGERHFIKHVLAFFAGSDGIVIENLAQRFMSDVKVPEARAFYGFQLMMENIHSETYSVL LDTYITDSEEKLRLLHAIQTIPCIQKKAEWAVRWIGSSASFQERLIGFAAVEGIFFSGSF CALFWLKKRGLMPGLTFSNELISRDEGLHTDFACLLYNSHIKHKLPRERVLEIIVDAVNI EREFICDALPVRLIGMNAELMAQYIEFVADRLLVSLGEEKHYHSTQPFEFMEMISLQGKT NFFERKVGEYQKAGVMSTEGTSKKFSLSEDF |
| DNA Sequence | >LmjF22.1290 |||ribonucleoside-diphosphate reductase small chain, putative|Leishmania major|chr 22|||Manual ATGTCTAGCG AGCCGAAGGC GATGGATACG TGCGCTGCGC CTGCGAAGCG TGCGCGCACGGAGGAGGCGG AGACGGAGGT TGCGCGGCCG TCCGGCGAGG CGGCCGGCGT GTCAAACCCGTCGACCGCGG AGGATCTTGT CACGAAACCA GTTGCGGCTG GCGCCGAGGT GCTGCCGGCGGACAAGGTTG CCGAAGGGAC GAACGCAGAG GAGGAGCCGC TGCAGCAGGA GAACCCGTTCCGCTACGTTC TCTTCCCAAT CCAGTACCAT GACATCTGGC GCAAGTACAA GGAGCAGGAGAGCTGCATCT GGACGGTCGA GGAGATCGAC CTGGGCAACG ACATGAAGGA CTGGGCAACGCTGAACGACG GGGAGCGGCA CTTCATCAAG CATGTGCTCG CCTTCTTCGC CGGCAGCGACGGCATAGTCA TCGAGAATCT CGCGCAGCGC TTCATGAGCG ACGTGAAGGT GCCAGAGGCACGCGCATTCT ACGGGTTCCA GCTGATGATG GAGAACATCC ACTCGGAGAC GTACTCTGTGCTGCTGGACA CGTACATCAC GGACAGCGAG GAGAAGCTGC GGCTGCTGCA CGCGATACAGACGATCCCGT GCATCCAGAA GAAGGCGGAG TGGGCCGTGC GGTGGATCGG GAGCAGCGCGAGCTTTCAGG AGCGGCTGAT CGGCTTTGCC GCGGTGGAGG GCATTTTCTT TTCCGGGTCGTTCTGCGCAC TGTTCTGGCT GAAGAAGCGC GGTCTGATGC CAGGGCTGAC GTTCAGCAACGAGCTCATCT CGCGCGACGA GGGTCTGCAC ACGGACTTCG CATGCCTGCT GTACAACTCGCACATCAAGC ACAAGCTGCC GCGCGAGCGC GTGTTGGAGA TTATCGTGGA TGCGGTGAACATCGAGCGCG AGTTCATCTG CGACGCGCTG CCGGTGCGGC TGATTGGCAT GAACGCGGAGCTGATGGCGC AGTACATCGA GTTCGTCGCG GACCGGCTGC TGGTGTCGCT CGGCGAGGAGAAGCACTACC ACTCGACGCA GCCGTTCGAA TTCATGGAGA TGATCTCGCT GCAGGGCAAAACAAACTTCT TCGAGAGGAA GGTGGGGGAG TACCAGAAGG CCGGCGTGAT GAGCACGGAGGGCACGTCGA AGAAGTTTTC CCTCTCGGAG GACTTCTAG |
| Ribonucleoside-diphosphate reductase small chain, putative Q4QBL8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ribonucleoside-diphosphate reductase activity |
| Biochemical Pathway | deoxyribonucleoside diphosphate metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K00526 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | ribonucleotide reductase M2 polypeptide [Homo sapiens] | 62 | 1e-120 | 429 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YJL026w ribonucleoside-diphosphate reductase, small subunit | Saccharomyces cerevisiae | 60% | 1e-113 | 401 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00317 | Ribonucleotide reductase small subunit signature | 170-186; | PS00368 | |
| Acylation | N-myristoylation site | 217-222; 234-239; 239-244; 251-256; 255-260; 375-380; | PS00008 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 384-387; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 41-44; 42-45; 67-70; 105-108; 120-123; 187-190; 221-224; 264-267; 336-339; 387-390; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 382-384; 383-385; | PS00005 | |
| Ribonucleoside-diphosphate reductase small chain, putative [Q4QBL8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1xsmA | |
| Percent Identity | 62% | |
| Target Region | 66-254 | |
| Template Region | 65-288 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| TYR | 113 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| FE (III) ION | 55.845 | Fe | [Fe+3] | 1xsm | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1060931/Q4QBL8.pdb 2.0 288 = residues | | = | +| Ramachandran plot: 93.5% core 4.9% allow 1.5% gener 0.0% = disall | | = | +| All Ramachandrans: 4 labelled residues (out of 286) = | +| Chi1-chi2 plots: 1 labelled residues (out of 198) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 3.0 Bad contacts: = 0 | *| Bond len/angle: 5.6 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.24 Covalent: -0.08 Overall: = 0.12 | | = | | M/c bond lengths: 99.4% within limits 0.6% highlighted = | | M/c bond angles: 95.7% within limits 4.3% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |