| Argininosuccinate synthase [Q4QBE8] | |
|---|---|
| Systematic Name | LmjF.23.0260 [Leishmania major] |
| Gene Name | LMJF_23_0260 |
| Molecular Weight | 46272 Da |
| Protein Sequence Size | 419 |
| Function | |
| Charge | -5.5 |
| Isoelectric Point | 5.7701 pH |
| Description | Argininosuccinate synthase (EC 6.3.4.5) (Citrulline--aspartate ligase). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 6.3.4.5 |
| Sequence | >tr|Q4QBE8|Q4QBE8_LEIMA Argininosuccinate synthase (EC 6.3.4.5) (Citrulline--aspartate ligase) - Leishmania major LATTTAIVCGQKPSVVLAYSGGLDTSVIIPWLKENYDYEVIACCANVGQGAGEIDGLEEK AKKAGASKLYLLDLREEYVTDYIFPTLKAGAMYEGKYMLGTSHARPLIAKHLVEVAHKEG AVAICHGATGKGNDQVRFELAIMALDPSLKCVAPWREWNIKSREDAIDYAEAHGVPVPCT KSDLYSRDRNLWHISHEGMDLEDPAKEPAHAKLLRLCNTVEKAPDEAEYVTVQFEKGIPV AVNGRKMASVELVEELNALGGKHAIGIEDIVEDRLVGMKSRGVYETPAGTILYKALDMLE SLCLDRDTQSFKRLSAVRFSELVYDGKWFTPLRESMSAMFDKMAETVTGEATLKLYKGNL MPAGAKSPYSLYDESIASFGDSHHLYNHHDAEGFIRLFGLPLRVRSMMLQAKEMPSSL |
| DNA Sequence | >LmjF23.0260 |||argininosuccinate synthase, putative|Leishmania major|chr 23|||Manual ATGCTCGCAA CGACGACGGC GATTGTGTGC GGCCAAAAGC CGAGTGTGGT GCTTGCGTACAGCGGCGGCC TGGACACGTC TGTAATCATC CCGTGGCTGA AGGAAAACTA CGACTACGAGGTGATTGCGT GCTGTGCAAA CGTTGGGCAG GGTGCCGGTG AGATAGACGG GCTGGAGGAAAAGGCGAAGA AGGCCGGTGC GTCGAAGCTG TACCTGCTGG ACCTGCGGGA GGAGTACGTGACGGACTACA TCTTCCCAAC GTTGAAGGCT GGCGCGATGT ACGAGGGGAA GTATATGCTGGGGACGTCGC ACGCGCGACC CCTGATTGCG AAGCACCTTG TGGAGGTTGC GCACAAGGAGGGCGCTGTCG CGATCTGCCA CGGCGCCACG GGGAAGGGCA ACGACCAAGT GCGGTTCGAGCTGGCGATCA TGGCGCTGGA CCCAAGCCTG AAGTGCGTGG CGCCGTGGCG GGAATGGAACATCAAGTCGC GCGAGGACGC GATCGACTAC GCGGAGGCCC ATGGCGTCCC TGTACCGTGCACGAAGTCTG ACCTTTACTC GCGTGACCGC AACCTGTGGC ACATCAGCCA CGAGGGCATGGACCTGGAGG ACCCCGCAAA GGAGCCGGCG CACGCGAAGC TTCTGAGGCT GTGCAACACGGTGGAGAAGG CGCCCGACGA GGCAGAGTAC GTGACGGTCC AGTTCGAGAA GGGCATCCCCGTCGCCGTGA ATGGCAGAAA AATGGCGAGC GTGGAGCTTG TGGAGGAGCT GAACGCGCTTGGCGGCAAGC ATGCGATCGG GATCGAGGAC ATCGTGGAGG ACCGCTTGGT AGGCATGAAGTCGCGCGGCG TGTATGAGAC GCCTGCAGGG ACGATCCTGT ACAAGGCGTT GGACATGCTGGAGTCGTTGT GTCTCGACCG TGACACGCAG TCCTTCAAGC GCCTGTCGGC AGTGCGCTTCTCCGAGCTTG TGTACGACGG CAAGTGGTTT ACGCCGCTGC GCGAGTCGAT GTCCGCGATGTTTGACAAGA TGGCGGAGAC GGTGACAGGC GAGGCGACAC TGAAGCTGTA CAAGGGTAACCTGATGCCTG CGGGTGCAAA GTCGCCGTAC TCGCTGTATG ACGAGAGCAT CGCATCGTTCGGCGACAGCC ACCACCTATA CAATCACCAC GATGCGGAGG GCTTCATCCG GCTGTTCGGGCTGCCGCTTC GTGTGCGCTC GATGATGCTG CAGGCAAAAG AGATGCCGAG CAGTTTGTGA |
| Argininosuccinate synthase Q4QBE8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; argininosuccinate synthase activity |
| Biochemical Pathway | arginine biosynthesis |
| Regulatory Pathway | |
| KEGG Pathways | K01940 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | argininosuccinate synthetase [Homo sapiens] | 45 | 4e-88 | 322 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| argG argininosuccinate synthase | Mycobacterium tuberculosis H37Rv | 39% | 5e-74 | 272 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00488 | Argininosuccinate synthase signatures | 19-27; | PS00564 | |
| PDOC00488 | Argininosuccinate synthase signatures | 128-139; | PS00565 | |
| Acylation | N-myristoylation site | 11-16; 22-27; 23-28; 49-54; 101-106; 238-243; 261-266; | PS00008 | |
| Amidation | Amidation site | 244-247; | PS00009 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 313-316; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 163-166; 181-184; 371-374; 379-382; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 87-89; 130-132; 149-151; 311-313; 353-355; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 162-170; 223-230; 319-325; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 32-46; 72-86; 163-177; | PS00003 | |
| Argininosuccinate synthase [Q4QBE8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1korD | |
| Percent Identity | 55% | |
| Target Region | 14-411 | |
| Template Region | 1-386 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ARG | 93 | Sidechain |
| ASP | 122 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | 506.196 | C10 H17 N6 O12 P3 | O=P(O)(O)NP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 1kor | |
| 7200-25-1 | ARGININE | 175.209 | C6 H15 N4 O2 | O=C(O)C(N)CCCN\C(=[NH2+])N | 1kor |
| 6283-68-7 | SUCCINIC ACID | 118.088 | C4 H6 O4 | O=C(O)CCC(=O)O | 1kor |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/231567/Q4QBE8.pdb 2.0 398 = residues | | = | +| Ramachandran plot: 92.3% core 6.9% allow 0.9% gener 0.0% = disall | | = | +| All Ramachandrans: 9 labelled residues (out of 396) = | +| Chi1-chi2 plots: 7 labelled residues (out of 237) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 3.7 Bad contacts: = 4 | *| Bond len/angle: 10.0 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.09 Covalent: -0.15 Overall: = 0.00 | | = | | M/c bond lengths: 98.8% within limits 1.2% highlighted = | *| M/c bond angles: 94.7% within limits 5.3% highlighted 2 off = graph | +| Planar groups: 99.3% within limits 0.7% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |