| Tryptophanyl-tRNA synthetase, putative [Q4QBE4] | |
|---|---|
| Systematic Name | LmjF.23.0300 [Leishmania major] |
| Gene Name | LMJF_23_0300 |
| Molecular Weight | 46256 Da |
| Protein Sequence Size | 412 |
| Function | |
| Charge | 11.5 |
| Isoelectric Point | 8.2 pH |
| Description | Tryptophanyl-tRNA synthetase, putative (EC 6.1.1.2). |
| Subcellular Location | cytosol[Predict] |
| E. C. Number | 6.1.1.2 |
| Sequence | >tr|Q4QBE4|Q4QBE4_LEIMA Tryptophanyl-tRNA synthetase, putative (EC 6.1.1.2) - Leishmania major DDGARQHMRDVMAKCRKRTMTTTPSANAEGVATPVLDAVPAGDAALQAAASPAMQQRQQP PALALHHFFHRDIAFSHRDLHKALVDIEASVKTGERSVFLYTGRGPSAGTMHLGHVLPFM LTKYLQDVFSLPLVIQITDDEKFLFRDVPFEGAKADELIRSNIKDIIAFNFNPRHTFIFR NTHYMGDMYPTVLRLQRSMTGNAVKHTLGITDSDNVGKLAFPATQAAPCFSTAFRRVLRN GDRPMRCLIPCAIDQDPFFVLTRAAALRLKQLPPALLHTKFLPALKGLEHKMSSSAEEKG VITLHDTDKQVRKKLRRAFSGGCATLEQMQETGANLELDVAYQYLRFFCPDDTLFADVTQ RYRSGTLNSGEVKDLAADCIIREVLHDWRERRATVTDDDVVEFCRIRDILL |
| DNA Sequence | >LmjF23.0300 |||tryptophanyl-tRNA synthetase, putative|Leishmania major|chr 23|||Manual ATGGACGACG GTGCGCGTCA GCACATGCGC GATGTCATGG CCAAGTGCCG TAAGCGGACCATGACCACCA CCCCATCCGC TAACGCCGAG GGCGTCGCGA CCCCGGTTTT GGATGCCGTGCCAGCTGGAG ATGCGGCACT GCAGGCCGCC GCATCTCCTG CCATGCAGCA GCGGCAGCAGCCACCAGCGC TGGCGCTTCA CCACTTCTTC CACCGCGACA TCGCCTTCTC ACACCGCGATTTGCACAAGG CACTTGTGGA TATCGAGGCC TCCGTCAAAA CGGGAGAGCG CTCTGTCTTCCTCTACACTG GCCGTGGCCC CAGCGCTGGC ACCATGCACT TGGGCCATGT GCTGCCCTTCATGCTCACCA AGTACCTCCA AGACGTCTTC AGCCTTCCGC TCGTTATACA AATCACAGATGACGAAAAGT TTCTCTTTCG CGACGTCCCC TTCGAGGGTG CCAAAGCGGA CGAGCTGATTCGCAGCAACA TCAAGGATAT CATCGCCTTC AACTTCAACC CGCGCCACAC CTTCATATTCCGCAACACGC ACTACATGGG CGATATGTAC CCGACAGTGC TCCGGCTGCA ACGTAGCATGACAGGCAACG CCGTGAAGCA CACGCTCGGC ATCACGGACA GCGATAATGT CGGCAAGCTTGCCTTTCCTG CCACCCAGGC TGCTCCGTGC TTCAGCACCG CTTTCCGTCG TGTTCTGCGGAACGGCGACA GGCCGATGCG CTGCCTGATT CCCTGCGCCA TTGATCAGGA CCCCTTCTTCGTTCTCACGC GCGCCGCCGC GCTGCGGCTG AAGCAGCTAC CGCCGGCCCT GCTTCACACCAAGTTCCTGC CGGCGCTCAA AGGCCTGGAG CACAAGATGA GCAGCAGTGC AGAGGAGAAGGGCGTCATTA CTCTACATGA CACCGATAAA CAGGTGCGCA AGAAGCTGCG CCGCGCCTTTTCGGGCGGCT GCGCCACCCT AGAGCAGATG CAAGAGACGG GCGCCAACCT GGAGTTGGATGTGGCGTACC AGTACCTGCG GTTCTTCTGC CCTGATGACA CACTCTTCGC CGACGTGACGCAGCGCTACC GTAGCGGTAC CCTGAATAGC GGAGAGGTGA AGGACCTGGC GGCGGATTGCATCATCCGTG AGGTGCTGCA CGATTGGCGG GAGCGGCGCG CAACGGTGAC GGACGATGACGTGGTGGAGT TCTGCCGTAT CCGTGACATT CTTCTCTGA |
| Tryptophanyl-tRNA synthetase, putative Q4QBE4] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; aminoacyl-tRNA ligase activity; tryptophan-tRNA ligase activity |
| Biochemical Pathway | tRNA aminoacylation for protein translation; tryptophanyl-tRNA aminoacylation |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | tryptophanyl-tRNA synthetase isoform b [Homo sapiens] | 44 | 2e-76 | 283 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YOL097c tryptophan--tRNA ligase | Saccharomyces cerevisiae | 45% | 5e-75 | 275 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00161 | Aminoacyl-transfer RNA synthetases class-I signature | 107-117; | PS00178 | |
| Acylation | N-myristoylation site | 210-215; 322-327; 366-371; | PS00008 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 17-20; 392-395; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 77-80; 139-142; 212-215; 295-298; 296-299; 304-307; 395-398; 397-400; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 77-79; 91-93; 103-105; 308-310; 360-362; | PS00005 | |
| Tryptophanyl-tRNA synthetase, putative [Q4QBE4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2azxB | |
| Percent Identity | 44% | |
| Target Region | 20-413 | |
| Template Region | 82-388 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| LYS | 273 | Sidechain |
| ALA | 278 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 8013-25-0 | GLYCEROL | 92.094 | C3 H8 O3 | OCC(O)CO | 2azx |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 2azx |
| 14808-79-8 | SULFATE ION | 96.063 | O4 S | [O-]S([O-])(=O)=O | 2azx |
| 6912-86-3 | TRYPTOPHAN | 204.225 | C11 H12 N2 O2 | O=C(O)C(N)Cc2c1ccccc1nc2 | 2azx |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/210920/Q4QBE4.pdb 2.0 393 = residues | | = | *| Ramachandran plot: 92.6% core 6.9% allow 0.3% gener 0.3% = disall | | = | +| All Ramachandrans: 5 labelled residues (out of 391) = | +| Chi1-chi2 plots: 2 labelled residues (out of 234) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 3 | *| Bond len/angle: 6.9 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.08 Covalent: -0.12 Overall: = 0.00 | | = | | M/c bond lengths: 99.5% within limits 0.5% highlighted = | | M/c bond angles: 94.4% within limits 5.6% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |