| Acetyl-CoA synthetase, putative [Q4QBC0] | |
|---|---|
| Systematic Name | LmjF.23.0540 [Leishmania major] |
| Gene Name | LMJF_23_0540 |
| Molecular Weight | 78522 Da |
| Protein Sequence Size | 705 |
| Function | |
| Charge | 2.5 |
| Isoelectric Point | 6.6886 pH |
| Description | Acetyl-CoA synthetase, putative (EC 6.2.1.1). |
| Subcellular Location | integral to membrane[Predict] |
| E. C. Number | 6.2.1.1 |
| Sequence | >tr|Q4QBC0|Q4QBC0_LEIMA Acetyl-CoA synthetase, putative (EC 6.2.1.1) - Leishmania major SDNPHSLHPLSSGSTSQSLHSSTKPPATQHVGEFNSVRDTNVVEPTEANRTKSHVGPHLG SRMRIYEYSIEHNDVFWAEIARRDFYWKTTWPDDQHVKSYNFDKSKGPIFVKWFEGAVTN ICYNALDRHLPAHKDRVCFYFEGNDPSVTEKVTYGSMYTRVVELANVLKHQYGITKGDRV GLYLPMIPFAAVAMLACARIGAVITVIFGGFSAQALSSRLKDAQAKLLITADASARGTKP ILLKDMADEALKVCEEEGMSIACLVFENMNRQFCKMKEGRDTWYGDALARLTPEQHEECP VEWMDAEDVLFLLYTSGSTGKPKAIVHTTAGYMVYASTTFMYSFDYHMDDVYFCTADIGW ITGHSYVVYGPMIHCATSVLFEGVANYPDYSRWWQLVEKYKVSILYTAPTAIRSLMQAGD DYVKVGNRSTLRVLGSVGEPINVEAWKWLRDVGGEGHCDVSDTWWQTETGGHMITPMPGC TPMKPGSATLPFFGVQPVILDPMKLNEKQGPAEGLLAIRAPWPGMARTIYGDHARFEKTY FDVDGYYMTGDGARRDSDGYYWITGRVDDVLNVSGHRIGTSEIEDAVNTHPAVVESAVVG FPHNIKGEGIYVFLTFRQGTEVTPELLAAVKATVRKVIGPLATPDVMQVARVGLPKTRSG KIVRRILRKVSSGQYTGLGDTSTLANPDVVEDLIAEHRRLCPRN |
| DNA Sequence | >LmjF23.0540 |||acetyl-CoA synthetase, putative|Leishmania major|chr 23|||Manual ATGAGCGACA ATCCTCACTC CCTGCATCCG CTGTCGTCGG GCTCGACAAG CCAATCATTGCACTCCTCGA CCAAGCCCCC CGCCACCCAG CATGTAGGCG AGTTTAACAG TGTGCGGGACACGAACGTTG TGGAGCCGAC GGAGGCAAAC CGGACAAAGT CGCACGTCGG CCCCCACCTTGGCTCCCGCA TGCGCATCTA CGAGTACTCC ATCGAGCACA ATGACGTCTT CTGGGCCGAAATTGCACGGC GCGACTTCTA CTGGAAGACG ACCTGGCCGG ATGACCAGCA CGTCAAGTCGTACAACTTCG ACAAATCCAA GGGCCCCATC TTTGTGAAAT GGTTCGAGGG CGCCGTGACAAACATCTGCT ACAACGCGTT GGACCGCCAC CTGCCGGCGC ACAAGGATCG CGTGTGCTTTTACTTTGAAG GCAACGACCC GAGTGTGACG GAAAAGGTCA CGTACGGCAG CATGTACACGAGGGTGGTGG AGCTGGCGAA CGTGCTGAAG CACCAGTACG GTATTACAAA GGGCGACCGCGTTGGCCTGT ATCTACCCAT GATCCCGTTC GCTGCAGTGG CCATGCTCGC CTGCGCCCGCATCGGTGCTG TCATCACCGT GATCTTCGGC GGTTTCTCGG CTCAAGCGCT CAGCTCACGTCTGAAAGACG CCCAGGCGAA GCTGCTTATC ACCGCCGACG CGTCGGCGCG GGGCACAAAGCCGATTCTTC TCAAGGACAT GGCAGACGAG GCGCTGAAGG TGTGTGAGGA GGAAGGCATGTCCATCGCGT GCCTCGTATT CGAAAACATG AACCGTCAAT TCTGCAAGAT GAAGGAAGGCCGCGATACCT GGTACGGCGA TGCCTTAGCG CGGCTGACGC CGGAGCAGCA CGAGGAGTGCCCGGTGGAGT GGATGGACGC GGAGGATGTG CTGTTCCTGC TCTACACCTC CGGCAGCACCGGGAAACCCA AGGCCATCGT GCACACGACG GCGGGCTACA TGGTGTACGC CTCCACCACCTTCATGTACA GCTTCGACTA CCACATGGAC GACGTGTACT TCTGCACGGC TGATATTGGCTGGATCACAG GCCACAGCTA CGTCGTGTAT GGTCCGATGA TCCACTGCGC CACGTCGGTGCTGTTCGAGG GTGTGGCGAA CTACCCCGAC TACTCGCGCT GGTGGCAGCT CGTCGAGAAGTACAAGGTGT CCATTCTCTA CACCGCGCCC ACCGCAATCC GCTCCCTCAT GCAGGCCGGCGACGACTACG TCAAGGTGGG CAACCGCAGC ACCCTGCGTG TGCTGGGCTC CGTCGGCGAGCCGATCAACG TGGAGGCGTG GAAGTGGCTG CGCGATGTCG GCGGCGAGGG CCACTGCGACGTGTCAGACA CGTGGTGGCA GACTGAGACG GGTGGCCACA TGATTACACC GATGCCGGGCTGCACCCCCA TGAAGCCCGG TAGTGCAACG CTGCCGTTCT TCGGTGTCCA GCCGGTTATTCTCGACCCCA TGAAGCTGAA TGAGAAGCAG GGCCCAGCGG AGGGCCTGCT CGCCATTCGTGCGCCGTGGC CAGGTATGGC CCGCACCATC TACGGCGACC ACGCACGCTT TGAGAAGACCTACTTTGACG TGGACGGGTA CTACATGACA GGCGACGGTG CTCGCCGCGA CTCGGACGGCTATTACTGGA TCACGGGCCG CGTCGATGAC GTGCTGAACG TGAGCGGGCA CCGCATCGGCACCAGCGAGA TCGAGGACGC CGTCAACACC CACCCGGCAG TGGTGGAGAG CGCCGTGGTCGGCTTCCCTC ACAACATCAA GGGTGAGGGC ATCTACGTCT TCCTTACGTT CCGCCAGGGGACGGAGGTGA CGCCCGAGCT GCTGGCGGCG GTAAAGGCGA CTGTGCGCAA GGTGATTGGTCCGCTGGCCA CACCGGACGT GATGCAGGTG GCTCGCGTCG GACTGCCGAA AACGCGCTCCGGCAAGATTG TGCGCCGCAT CCTGCGCAAG GTATCCAGTG GGCAGTACAC GGGGCTGGGCGACACCTCGA CGCTGGCGAA CCCCGACGTT GTTGAGGACC TCATCGCCGA GCACCGGAGGCTGTGCCCTC GCAACTAG |
| Acetyl-CoA synthetase, putative Q4QBC0] | |
|---|---|
| Metabolite Information | |
| Molecular Function | AMP binding; acetate-CoA ligase activity; catalytic activity |
| Biochemical Pathway | metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K01895 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | acyl-CoA synthetase short-chain family member 2 isoform a [Homo sapiens] | 52 | 0 | 701 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| acsA Acetyl-CoA synthetase | Staphylococcus aureus | 35% | 1e-90 | 328 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00427 | Putative AMP-binding domain signature | 313-324; | PS00455 | |
| Acylation | N-myristoylation site | 117-122; 144-149; 156-161; 174-179; 202-207; 210-215; 259-264; 427-432; 525-530; 654-659; 674-679; 678-683; | PS00008 | |
| Glycosylation | N-glycosylation site | 50-53; 428-431; 573-576; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 555-558; 669-672; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 37-40; 91-94; 148-151; 176-179; 437-440; 540-543; 582-585; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 23-25; 37-39; 150-152; 218-220; 235-237; 320-322; 431-433; 565-567; 616-618; 634-636; 660-662; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 278-285; 539-547; 555-562; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 540-554; | PS00003 | |
| Acetyl-CoA synthetase, putative [Q4QBC0] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2p2fB | |
| Percent Identity | 52% | |
| Target Region | 1-705 | |
| Template Region | 5-632 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 71-50-1 | ACETATE ION | 59.044 | C2 H3 O2 | [O-]C(=O)C | 2p2f |
| 61-19-8 | ADENOSINE MONOPHOSPHATE  | 347.221 | C10 H14 N5 O7 P   | O=P(O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 2p2f |
| 31416-98-5 | COENZYME A | 767.534 | C21 H36 N7 O16 P3 S | O=C(NCCS)CCNC(=O)C(O)C(C)(C)COP(=O)(O)OP(=O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3OP(=O)(O)O | 2p2f |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
|
|
|
| Modeled Protein | Template Structure |
|
|
+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/1087960/Q4QBC0.pdb 2.0 705 = residues | | = | *| Ramachandran plot: 91.1% core 7.9% allow 0.8% gener 0.2% = disall | | = | +| All Ramachandrans: 16 labelled residues (out of 703) = | +| Chi1-chi2 plots: 5 labelled residues (out of 387) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 9.1 Bad contacts: = 8 | *| Bond len/angle: 8.0 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: -0.03 Covalent: -0.20 Overall: = -0.09 | | = | | M/c bond lengths: 98.9% within limits 1.1% highlighted = | | M/c bond angles: 92.6% within limits 7.4% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |