| Aspartate aminotransferase, putative [Q4QAU4] | |
|---|---|
| Systematic Name | LmjF.24.0370 [Leishmania major] |
| Gene Name | LMJF_24_0370 |
| Molecular Weight | 47653 Da |
| Protein Sequence Size | 431 |
| Function | |
| Charge | 9.5 |
| Isoelectric Point | 7.9 pH |
| Description | Aspartate aminotransferase, putative (EC 2.6.1.1). |
| Subcellular Location | Mitochondrion[Predict] |
| E. C. Number | 2.6.1.1 |
| Sequence | >tr|Q4QAU4|Q4QAU4_LEIMA Aspartate aminotransferase, putative (EC 2.6.1.1) - Leishmania major LRHVCSSAVVAYSAGLAPVSCRRDGSTSYFSAVPRAPPDAIMGIAADFAKDMCPSKVNLC IGVYRDEQNKPFVLESVRKAMSHIVERDTQMDYAPIAGLPSFVNSVQRLCFGKPMLDVQG DRIASAQTLSGTGALHLGVQLLQRSSGGSGTATLHIPSPSYPNHLNILQHLNVEASYYPY YNLNTHRLNIEAMLNYLRQLPAGSVVLLHACAHNPTGCDPTPEEWQQIVDVICRSDLIPF VDMAYQGFATGDIERDAYVLRALNQQEVPTYLVAQSLAKSFGLYGHRTGAVHIRCTTQKE KANVLSQLQSTIRATYSNPPIFGARIADEILRTPQLRELWKSELNQMSSRLQDVRHRLVA QLRACGSTRDWEYLEKGVGMMSLTGLTEEQVMALQQKYSVYLTRNGRIAFSGLSSENVAY VAQSIHDVSR |
| DNA Sequence | >LmjF24.0370 |||aspartate aminotransferase, putative|Leishmania major|chr 24|||Manual ATGCTTCGTC ACGTGTGCTC GTCCGCCGTC GTGGCCTACA GTGCTGGCCT GGCGCCGGTCAGCTGCCGCC GTGACGGGTC GACGAGCTAC TTCTCGGCCG TGCCGCGCGC GCCTCCAGATGCCATCATGG GCATTGCTGC GGACTTTGCC AAGGACATGT GCCCAAGCAA GGTAAACCTTTGCATCGGAG TCTACCGTGA TGAGCAGAAC AAGCCCTTTG TGCTGGAGTC TGTGCGCAAGGCCATGTCGC ACATTGTGGA GCGCGACACG CAGATGGACT ATGCACCCAT TGCGGGTCTGCCGTCCTTCG TGAATAGCGT GCAGCGGCTA TGCTTTGGAA AGCCAATGCT GGACGTTCAAGGGGACCGCA TCGCCTCGGC ACAAACTCTT AGTGGCACCG GTGCCCTGCA CCTCGGCGTGCAGCTACTGC AGCGCTCCTC TGGTGGCTCC GGCACCGCGA CGCTGCACAT CCCGAGCCCCTCCTACCCGA ATCACCTGAA TATCCTGCAG CATCTAAACG TTGAGGCTAG CTATTACCCGTACTACAATC TGAACACGCA TCGCTTGAAC ATCGAGGCGA TGCTCAACTA TCTGCGCCAGCTGCCCGCCG GCTCCGTGGT CCTGCTGCAC GCCTGTGCGC ACAACCCGAC TGGGTGCGACCCTACTCCAG AGGAATGGCA GCAGATTGTA GACGTCATCT GCCGAAGCGA TCTCATTCCGTTCGTTGACA TGGCATACCA GGGTTTTGCG ACGGGTGACA TCGAGCGTGA CGCCTACGTCCTACGCGCGT TGAATCAGCA AGAGGTCCCC ACGTACTTGG TGGCCCAGTC GTTGGCGAAGAGCTTTGGTC TCTACGGGCA TCGCACTGGC GCCGTCCACA TCCGCTGCAC CACGCAGAAGGAGAAGGCAA ATGTCCTGTC GCAGCTGCAG TCGACGATTC GCGCCACCTA CAGCAACCCTCCCATCTTCG GTGCTCGCAT CGCGGACGAA ATTTTGCGAA CCCCGCAGCT GCGCGAGCTGTGGAAGTCGG AGCTGAACCA GATGTCGAGC CGACTGCAGG ATGTTCGGCA CCGCCTCGTGGCCCAGCTGC GTGCTTGTGG CTCGACGCGC GACTGGGAGT ACCTCGAGAA AGGTGTGGGTATGATGTCGC TGACGGGGCT GACGGAGGAG CAGGTGATGG CGCTGCAGCA GAAGTACAGCGTCTACCTGA CGCGCAACGG TCGCATCGCA TTTTCTGGAC TGAGCTCCGA AAATGTGGCCTACGTCGCTC AGTCCATCCA CGATGTGTCC CGGTAG |
| Aspartate aminotransferase, putative Q4QAU4] | |
|---|---|
| Metabolite Information | |
| Molecular Function | alanine transaminase activity; transaminase activity; transferase activity, transferring nitrogenous groups |
| Biochemical Pathway | amino acid metabolism; biosynthesis; methionine salvage |
| Regulatory Pathway | |
| KEGG Pathways | K00813 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | aspartate aminotransferase 2 precursor [Homo sapiens] | 40 | 2e-89 | 327 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| aspC aspartate aminotransferase | Haemophilus influenzae | 35% | 1e-72 | 268 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| Acylation | N-myristoylation site | 148-153; 149-154; 324-329; | PS00008 | |
| Phosphorylation | Casein kinase II phosphorylation site | 90-93; 217-220; 222-225; 298-301; 368-371; 425-428; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 21-23; 77-79; 186-188; 298-300; 312-314; 349-351; 368-370; | PS00005 | |
| Aspartate aminotransferase, putative [Q4QAU4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 9aatB | |
| Percent Identity | 40% | |
| Target Region | 8-431 | |
| Template Region | 3-401 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| TYR | 135 | Sidechain (TRP 140 In template) |
| ASP | 216 | Sidechain |
| LYS | 253 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE | 248.173 | C8 H13 N2 O5 P | O=P(O)(O)OCc1cnc(c(O)c1CN)C | 9aat | |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/557600/Q4QAU4.pdb 2.0 404 = residues | | = | *| Ramachandran plot: 93.3% core 5.9% allow 0.6% gener 0.3% = disall | | = | +| All Ramachandrans: 5 labelled residues (out of 402) = | +| Chi1-chi2 plots: 4 labelled residues (out of 232) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 11.9 Bad contacts: = 4 | *| Bond len/angle: 6.1 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | | G-factors Dihedrals: 0.14 Covalent: -0.12 Overall: = 0.04 | | = | | M/c bond lengths: 99.2% within limits 0.8% highlighted = | | M/c bond angles: 93.9% within limits 6.1% highlighted = | +| Planar groups: 99.3% within limits 0.7% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |