| Triosephosphate isomerase [Q4QAP8] | |
|---|---|
| Systematic Name | LmjF.24.0850 [Leishmania major] |
| Gene Name | LMJF_24_0850 |
| Molecular Weight | 27195 Da |
| Protein Sequence Size | 251 |
| Function | |
| Charge | 5.5 |
| Isoelectric Point | 8.6018 pH |
| Description | Triosephosphate isomerase (EC 5.3.1.1) (TIM) (Triose-phosphate isomerase). |
| Subcellular Location | glycosome[Predict] |
| E. C. Number | 5.3.1.1 |
| Sequence | >tr|Q4QAP8|Q4QAP8_LEIMA Triosephosphate isomerase (EC 5.3.1.1) (TIM) (Triose-phosphate isomerase) - Leishmania major SAKPQPIAAANWKCNGTTASIEKLVQVLNEHNISHDVQCVVAPTFVHIPLVQAKLRNPKY VVSAENAIAKSGAFTGEVSMPILKDLGINWVILGHSERRTYYGETDETVAQKVADACKQG FMVIACIGETLQQREANQTAKVVLSQTSAIATKLPKEAWDQIVLAYEPVWAIGTGKVATP EQAQEVHALLRKWVSEKIGTDVAAKLRILYGGSVSAGNAKTLYMKPDINGFLVGGASLKP EFRDIIDATR |
| DNA Sequence | >LmjF24.0850 |||triosephosphate isomerase|Leishmania major|chr 24|||Manual ATGTCCGCCA AGCCGCAGCC GATCGCTGCT GCGAACTGGA AGTGCAACGG CACCACCGCCTCTATCGAGA AGCTGGTGCA GGTGCTCAAT GAACACAACA TCTCCCATGA CGTGCAGTGCGTCGTGGCAC CGACCTTTGT GCACATCCCA TTGGTTCAGG CGAAGCTCCG CAACCCCAAGTACGTCGTCA GCGCCGAGAA CGCGATCGCC AAGAGCGGCG CCTTCACCGG TGAAGTGTCAATGCCGATCC TGAAGGACCT GGGCATCAAC TGGGTCATTC TTGGCCACTC GGAGCGCCGCACCTACTACG GTGAGACGGA TGAGACCGTA GCGCAAAAAG TTGCGGATGC GTGCAAGCAGGGCTTCATGG TGATTGCTTG CATTGGAGAG ACGCTGCAGC AGCGTGAAGC GAACCAGACAGCGAAGGTGG TGTTGAGCCA GACGTCAGCG ATCGCCACGA AGCTGCCGAA GGAGGCGTGGGACCAGATTG TGCTCGCCTA CGAGCCGGTA TGGGCCATCG GCACCGGCAA GGTCGCCACGCCCGAGCAGG CGCAGGAGGT GCACGCCCTG TTGCGCAAGT GGGTGAGCGA GAAGATCGGCACCGACGTCG CCGCGAAGCT CCGCATCCTG TACGGCGGCT CTGTCAGCGC CGGCAATGCCAAGACGCTCT ACATGAAGCC TGATATCAAC GGCTTCCTGG TCGGTGGCGC GTCCCTGAAGCCGGAGTTCC GAGACATCAT CGATGCAACC CGGTAA |
| Triosephosphate isomerase Q4QAP8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | triose-phosphate isomerase activity |
| Biochemical Pathway | glycolysis; metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K01803 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | triosephosphate isomerase 1 [Homo sapiens] | 52 | 1e-64 | 243 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| tpiA triosephosphate isomerase | Haemophilus influenzae | 42% | 2e-47 | 182 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00155 | Triosephosphate isomerase active site | 166-176; | PS00171 | |
| Acylation | N-myristoylation site | 17-22; 73-78; 200-205; 212-217; 213-218; 218-223; | PS00008 | |
| Glycosylation | N-glycosylation site | 16-19; 33-36; 138-141; | PS00001 | |
| Phosphorylation | Protein kinase C phosphorylation site | 2-4; 97-99; 140-142; 175-177; 196-198; 238-240; | PS00005 | |
| Triosephosphate isomerase [Q4QAP8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1amkA | |
| Percent Identity | 92% | |
| Target Region | 1-251 | |
| Template Region | 1-250 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASN | 12 | Sidechain |
| LYS | 14 | Sidechain |
| HIS | 96 | Sidechain |
| GLU | 168 | Sidechain |
| GLY | 174 | Backbone amide |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 13147-57-4 | 2-PHOSPHOGLYCOLIC ACID | 156.031 | C2 H5 O6 P | O=P(O)(O)OCC(=O)O | 1amk |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/229782/Q4QAP8.pdb 2.0 251 = residues | | = | +| Ramachandran plot: 95.9% core 3.6% allow 0.5% gener 0.0% = disall | | = | +| All Ramachandrans: 2 labelled residues (out of 249) = | +| Chi1-chi2 plots: 3 labelled residues (out of 135) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | | Residue properties: Max.deviation: 2.5 Bad contacts: = 2 | +| Bond len/angle: 4.7 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.06 Covalent: -0.11 Overall: = 0.00 | | = | | M/c bond lengths: 99.5% within limits 0.5% highlighted = | | M/c bond angles: 94.7% within limits 5.3% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |