| Succinate dehydrogenase flavoprotein, putative [Q4QAG8] | |
|---|---|
| Systematic Name | LmjF.24.1630 [Leishmania major] |
| Gene Name | LMJF_24_1630 |
| Molecular Weight | 66736 Da |
| Protein Sequence Size | 607 |
| Function | |
| Charge | 5 |
| Isoelectric Point | 6.8935 pH |
| Description | Succinate dehydrogenase flavoprotein, putative. |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QAG8|Q4QAG8_LEIMA Succinate dehydrogenase flavoprotein, putative - Leishmania major. LRRTFTRLALSRAYPVIDHTYDCVVVGAGGSGLRAAMGVAASGYDVACISKLYPSRSHTI AAQGGINAALANCEEDDWRWHVYDTVKGSDWLGDQDAIHYMCQEAPCVVSELESMGLPFL RTKDGFIYQRAFGGQSIHYGGKQARRTCAASDRTGHAMLHTLYGQSFQYGVNFYNEYYCL DLIMEDGCCRGVMAMSIDDGTIHRFKSKYTVLCTGGYGRVYFTTTSAKSCTGDGTAMVAR AGLPAEDMEFVQFHPTGIYGPGVLITEGARGEGGYLVNSEGERFMERYAPKAKDLASRDV VSRAITMEILAGRGCGPKKDHVMLQLHHLDPATLHKKLPGISESAHIFAGVDVTKEPIPI VPTVHYSMGGVPTLWTGEVVSPRNGDDNAIVPGLLAAGECACASVHGANRLGANSLLDIV VFGKSCANTVIFNLTKEGRKQPELRADAGEASIADLDRILTNKGDIPIARIRERMKETMA LYAAVFRTEENMQTGKKIIEECYRDFSHAFVHDKSPVWNSNLIEALELRNLLTNAMMTIS GAVVRKESRGAHARDDYQERDDHNWMKHTLAYLDDANGKVRLAYRPVHMEMLTNEVESIP PAKRVY |
| DNA Sequence | >LmjF24.1630 |||succinate dehydrogenase flavoprotein, putative|Leishmania major|chr 24|||Manual ATGCTGCGCC GCACCTTTAC GCGTCTTGCC CTTAGCCGCG CCTACCCGGT CATCGACCACACGTACGACT GCGTCGTCGT GGGCGCCGGT GGCTCTGGTC TCCGCGCGGC CATGGGTGTGGCGGCCTCTG GCTACGATGT GGCGTGCATC TCGAAGCTGT ACCCGTCTCG CTCGCACACGATAGCGGCGC AGGGTGGCAT TAACGCTGCT CTCGCCAACT GCGAGGAGGA TGACTGGCGCTGGCATGTAT ACGACACAGT AAAGGGCTCG GACTGGCTTG GCGACCAGGA TGCAATCCATTACATGTGCC AGGAGGCCCC ATGCGTTGTG TCGGAGCTGG AGAGCATGGG TCTGCCCTTCCTGCGCACAA AGGATGGCTT CATCTACCAG CGCGCCTTTG GTGGCCAGTC GATCCACTACGGTGGCAAGC AGGCGCGCCG CACCTGTGCG GCGTCGGACC GCACGGGCCA CGCCATGCTTCACACCCTGT ACGGCCAGTC CTTCCAATAC GGCGTGAACT TTTACAACGA ATACTACTGCCTCGATCTGA TCATGGAGGA TGGCTGCTGC CGCGGTGTGA TGGCCATGAG CATCGACGATGGCACGATCC ACCGCTTCAA GTCCAAGTAC ACGGTGCTCT GCACGGGTGG CTACGGCCGCGTATACTTCA CGACGACAAG CGCGAAGAGC TGCACAGGCG ACGGCACGGC CATGGTGGCGCGCGCCGGTC TGCCGGCGGA GGACATGGAG TTTGTGCAAT TCCATCCCAC CGGCATCTACGGCCCTGGCG TGCTCATCAC CGAGGGCGCG CGCGGCGAGG GTGGCTACCT TGTGAACAGCGAGGGTGAGC GCTTCATGGA GCGCTACGCC CCCAAGGCGA AGGATCTGGC GTCGCGCGATGTCGTGTCGC GGGCCATTAC GATGGAGATC CTAGCGGGTC GCGGCTGCGG TCCCAAGAAGGACCACGTGA TGCTGCAGCT ACACCACCTC GACCCTGCCA CTCTGCATAA GAAGCTGCCGGGCATCTCAG AGAGCGCTCA CATCTTCGCC GGCGTTGACG TGACCAAGGA GCCGATTCCGATTGTGCCAA CGGTGCACTA CTCCATGGGC GGCGTGCCGA CACTGTGGAC GGGCGAGGTGGTCAGCCCGC GCAACGGTGA CGACAACGCG ATTGTTCCCG GCCTTCTCGC AGCTGGTGAGTGCGCCTGTG CTAGCGTGCA CGGCGCCAAC CGCCTCGGCG CGAACTCGCT GCTGGACATTGTCGTGTTCG GCAAGTCGTG TGCCAACACG GTCATCTTTA ATCTGACGAA GGAGGGCCGCAAGCAGCCCG AGCTGCGCGC CGACGCCGGC GAGGCCTCGA TCGCCGATCT GGACCGCATCCTCACTAACA AGGGCGACAT TCCCATCGCC CGTATCCGTG AGCGTATGAA GGAGACGATGGCGCTCTACG CCGCCGTCTT CCGCACAGAG GAGAACATGC AAACAGGAAA GAAGATAATCGAGGAGTGCT ACCGCGACTT CTCTCACGCC TTCGTGCACG ATAAATCGCC GGTGTGGAACAGCAACCTCA TCGAGGCGCT CGAACTGCGC AACCTGCTGA CGAACGCTAT GATGACGATTAGTGGCGCTG TGGTGCGCAA GGAGAGCCGC GGTGCCCACG CACGTGACGA CTACCAAGAGCGTGACGACC ACAACTGGAT GAAGCACACT CTCGCATACC TCGACGACGC CAACGGCAAGGTGCGCCTTG CGTACCGCCC GGTGCACATG GAGATGCTGA CGAATGAGGT GGAGAGCATTCCGCCGGCGA AGCGAGTGTA CTAG |
| Succinate dehydrogenase flavoprotein, putative Q4QAG8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | FAD binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-CH group of donors; succinate dehydrogenase activity |
| Biochemical Pathway | electron transport; tricarboxylic acid cycle |
| Regulatory Pathway | |
| KEGG Pathways | K00239 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | succinate dehydrogenase complex, subunit A, flavoprotein precursor [Homo sapiens] | 59 | 0 | 746 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| nadB L-aspartate oxidase | Mycobacterium tuberculosis H37Rv | 30% | 4e-44 | 173 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00393 | Fumarate reductase / succinate dehydrogenase FAD-binding site | 57-66; | PS00504 | |
| Acylation | N-myristoylation site | 28-33; 33-38; 39-44; 65-70; 66-71; 141-146; 188-193; 263-268; 341-346; 351-356; 370-375; 394-399; | PS00008 | |
| Amidation | Amidation site | 438-441; 495-498; | PS00009 | |
| Glycosylation | N-glycosylation site | 434-437; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 546-549; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 43-46; 111-114; 197-200; 280-283; 416-419; 453-456; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 86-88; 152-154; 227-229; 382-384; 462-464; 495-497; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 122-129; 497-504; | PS00007 | |
| Succinate dehydrogenase flavoprotein, putative [Q4QAG8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2h89A | |
| Percent Identity | 60% | |
| Target Region | 12-626 | |
| Template Region | 9-613 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| HIS | 244 | Sidechain |
| ARG | 288 | Sidechain |
| HIS | 355 | Sidechain |
| ARG | 400 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 2-HEXYLOXY-6-HYDROXYMETHYL-TETRAHYDRO-PYRAN- 3,4,5-TRIOL | 264.315 | C12 H24 O6 | O(CCCCCC)C1OC(C(O)C(O)C1O)CO | 2h89 | |
| FE3-S4 CLUSTER | 295.795 | Fe3 S4 | S1[Fe]2S[Fe]3[S]2[Fe]1S3 | 2h89 | |
| 16426-55-4 | FLAVIN-ADENINE DINUCLEOTIDE | 785.55 | C27 H33 N9 O15 P2 | O=C2C3=Nc1cc(c(cc1N(C3=NC(=O)N2)CC(O)C(O)C(O)COP(=O)(O)OP(=O)(O)OCC6OC(n5cnc4c(ncnc45)N)C(O)C6O)C)C | 2h89 |
| FE2/S2 (INORGANIC) CLUSTER | 175.82 | Fe2 S2 | [Fe]1S[Fe]S1 | 2h89 | |
| 104414-01-9 | PROTOPORPHYRIN IX CONTAINING FE | 616.487 | C34 H32 Fe N4 O4 | Cc1c2n3c(c1C=C)C=C4C(=C(C5=[N]4[Fe]36[N]7=C(C=C8N6C(=C5)C(=C8C)CCC(=O)O)C(=C(C7=C2)C=C)C)CCC(=O)O)C | 2h89 |
| 24203-36-9 | POTASSIUM ION | 39.098 | K | [K+] | 2h89 |
| MALONATE ION | 102.046 | C3 H2 O4 | [O-]C(=O)CC([O-])=O | 2h89 | |
| 39382-08-6 | PHOSPHATIDYLETHANOLAMINE | 749.073 | C41 H83 N O8 P | O=C(OCC(OC(=O)CCCCCCCCCCCCCCCCC)COP(=O)(OCC[NH3+])O)CCCCCCCCCCCCCCCCC | 2h89 |
| IRON/SULFUR CLUSTER | 351.64 | Fe4 S4 | [S]12[Fe]3[S]4[Fe]1[S]5[Fe]2[S]3[Fe]45 | 2h89 | |
| Mutational Information | ||
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| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/484700/Q4QAG8.pdb 2.0 596 = residues | | = | +| Ramachandran plot: 89.9% core 9.7% allow 0.4% gener 0.0% = disall | | = | +| All Ramachandrans: 12 labelled residues (out of 594) = | +| Chi1-chi2 plots: 2 labelled residues (out of 332) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 3.1 Bad contacts: = 3 | *| Bond len/angle: 8.5 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.03 Covalent: -0.14 Overall: = -0.03 | | = | | M/c bond lengths: 99.5% within limits 0.5% highlighted = | *| M/c bond angles: 94.5% within limits 5.5% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |