| Transketolase, putative [Q4QAC4] | |
|---|---|
| Systematic Name | LmjF.24.2060 [Leishmania major] |
| Gene Name | LMJF_24_2060 |
| Molecular Weight | 71667 Da |
| Protein Sequence Size | 671 |
| Function | |
| Charge | 0 |
| Isoelectric Point | 6.5067 pH |
| Description | Transketolase, putative. |
| Subcellular Location | cytoplasm[Predict] |
| E. C. Number | N.A. |
| Sequence | >tr|Q4QAC4|Q4QAC4_LEIMA Transketolase, putative - Leishmania major. AAIEKVANCIRCLAADIVQGGKSGHPGTPMGMAPVSAVLWTEVMKYNSQDPNWVDRDRFI MSNGHGCALHYALLHMAGYDLTMDDLKGFRQYGSRTPGHPERFVTPGVEVTTGPLGQGIA NAVGLAMAESHLAATFNRPGHELVNHYTYVYCGDGCLMEGVCQEALSLAGHLALEKLIII YDSNYICIDGATNLSFTEQSHQKYVAMGFHVIEVENGDTDYDGLRKALAEAKATKGKPKM IVQTTTIGFGSSKQGTEKVHGAPLGEEDIANVKTKFGRDPNKKYDVDEDVRAVFRTHVEK CSAEQRAWEERLAKYTAAFPAEGAAFVAQMKGELPPGWEAKLPTNSSAIATRKASENCLA ALFPAIPALMGGSADLTPSNLTRPASANLVDFSSTSQEGRYIRFGVREHAMCAILNGLDA HGGVIPFGGTFLNFIGYALGAVRLAAISHHRVIYVATHDSIGVGEDGPTHQPVELVAALR AMPNLQVIRPSDQTETSGAWAVALSSAHTPTVLCLSRQNTVPQSGSSIEGVKRGAYAVVE VSDPQLVIVASGSEVSLAVDAAKALSGELCVRVVSMPCQELFDAQTDVYRKSVLAEGVPV VSVEAYVSFGWEKYSHAHVGMSGFGASAPAGVLYNKFGITVEEVVETARRLAARFPNGTA PLKNSSFSKM |
| DNA Sequence | >LmjF24.2060 |||transketolase, putative|Leishmania major|chr 24|||Manual ATGGCCGCCA TTGAAAAAGT TGCTAACTGC ATCCGCTGCC TCGCGGCGGA CATTGTGCAGGGCGGCAAGA GTGGCCACCC AGGCACGCCG ATGGGCATGG CGCCAGTGTC AGCGGTCCTGTGGACGGAAG TGATGAAGTA CAACAGCCAG GACCCTAACT GGGTCGACCG CGACCGCTTCATCATGTCGA ACGGGCACGG TTGCGCGCTG CATTACGCCT TGCTGCACAT GGCCGGCTACGACCTTACCA TGGACGACCT GAAGGGATTC CGCCAATATG GCTCCCGCAC CCCTGGCCACCCCGAGCGTT TCGTGACGCC CGGGGTGGAG GTGACGACCG GGCCGCTTGG TCAGGGTATTGCAAACGCGG TCGGACTGGC GATGGCCGAG TCGCACCTTG CCGCCACGTT CAACCGCCCGGGACACGAGC TCGTCAATCA TTACACTTAC GTGTACTGTG GTGATGGTTG TCTGATGGAGGGTGTGTGCC AGGAGGCGCT CTCCCTCGCC GGCCACCTCG CCCTGGAGAA GCTCATCATCATCTATGACA GCAACTACAT CTGCATCGAT GGCGCGACAA ACCTCTCCTT CACAGAACAGTCTCACCAGA AGTACGTGGC CATGGGTTTC CATGTGATCG AGGTCGAAAA CGGTGACACTGACTACGACG GCCTGCGCAA GGCATTGGCG GAGGCCAAGG CCACGAAGGG GAAGCCAAAGATGATTGTGC AAACCACAAC GATCGGGTTC GGGTCTTCGA AGCAGGGAAC GGAGAAAGTGCACGGCGCGC CGCTGGGTGA AGAGGATATT GCAAACGTCA AGACAAAGTT TGGCCGCGACCCGAACAAGA AGTACGACGT CGACGAAGAC GTCCGCGCTG TGTTCAGGAC GCACGTGGAGAAGTGCTCGG CGGAGCAGAG GGCATGGGAG GAACGCTTGG CGAAGTACAC GGCTGCTTTCCCGGCCGAGG GTGCCGCCTT TGTGGCGCAG ATGAAGGGCG AGCTGCCGCC CGGGTGGGAGGCGAAGCTGC CGACGAACTC CTCGGCCATC GCAACGCGCA AGGCGAGTGA GAACTGCCTGGCTGCGCTCT TCCCGGCCAT CCCGGCCCTC ATGGGCGGGT CAGCTGACCT CACGCCGAGCAACCTGACGC GCCCCGCGTC GGCAAACTTG GTGGACTTCT CGTCGACCAG CCAAGAGGGTCGCTACATTC GCTTCGGTGT CCGCGAACAC GCCATGTGCG CCATCCTCAA CGGTCTCGACGCTCATGGTG GTGTCATCCC GTTCGGCGGC ACCTTCCTCA ACTTCATCGG CTACGCCCTTGGTGCAGTGC GCCTCGCCGC GATCTCTCAC CACCGCGTCA TCTACGTGGC GACACACGACAGCATCGGGG TTGGCGAGGA CGGGCCGACC CACCAACCGG TCGAGTTGGT GGCTGCCCTGCGTGCCATGC CGAACCTGCA GGTGATACGT CCTAGCGACC AGACAGAGAC GAGCGGTGCGTGGGCTGTTG CGTTGTCCAG TGCCCACACC CCGACGGTTC TATGTCTGAG CCGCCAGAACACCGTGCCGC AGTCGGGGTC GAGCATCGAA GGTGTGAAGC GCGGTGCGTA CGCAGTGGTGGAGGTGTCCG ACCCTCAGCT CGTGATCGTG GCGAGCGGCT CGGAGGTGTC GCTGGCGGTGGATGCTGCCA AAGCACTCTC GGGTGAGCTG TGTGTGAGGG TCGTGTCGAT GCCGTGCCAGGAGCTCTTCG ACGCGCAAAC GGATGTGTAC CGCAAGTCTG TGCTCGCCGA AGGTGTGCCGGTGGTGTCGG TGGAGGCGTA CGTCAGCTTC GGCTGGGAGA AATACTCCCA TGCACACGTGGGCATGTCTG GCTTCGGTGC CTCGGCCCCG GCGGGTGTGC TTTACAATAA GTTCGGCATTACCGTGGAGG AAGTGGTGGA GACGGCCCGC AGGCTGGCCG CGCGCTTCCC CAATGGCACGGCGCCGCTCA AGAACTCCTC ATTCAGCAAG ATGTAA |
| Transketolase, putative Q4QAC4] | |
|---|---|
| Metabolite Information | |
| Molecular Function | transketolase activity |
| Biochemical Pathway | pentose-phosphate shunt |
| Regulatory Pathway | |
| KEGG Pathways | K00615 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | transketolase [Homo sapiens] | 25 | 2e-36 | 151 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| tktA transketolase 1 | Haemophilus influenzae | 47% | 1e-165 | 576 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00299 | Microbodies C-terminal targeting signal | 669-671; | PS00342 | |
| PDOC00635 | Transketolase signatures | 12-32; | PS00801 | |
| PDOC00635 | Transketolase signatures | 465-481; | PS00802 | |
| Acylation | N-myristoylation site | 21-26; 28-33; 108-113; 117-122; 119-124; 125-130; 224-229; 262-267; 430-435; 531-536; 553-558; 632-637; | PS00008 | |
| Glycosylation | N-glycosylation site | 194-197; 346-349; 381-384; 658-661; 665-668; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 353-356; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 83-86; 196-199; 220-223; 297-300; 396-399; 527-530; 552-555; 641-644; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 252-254; 257-259; 352-354; 648-650; | PS00005 | |
| Transketolase, putative [Q4QAC4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1r9jB | |
| Percent Identity | 89% | |
| Target Region | 1-671 | |
| Template Region | 1-671 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| HIS | 26 | Sidechain |
| GLY | 251 | Unknown |
| SER | 253 | Unknown |
| GLN | 255 | Unknown |
| HIS | 261 | Sidechain |
| GLU | 409 | Unknown |
| HIS | 471 | Unknown |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 17787-72-3 | CALCIUM ION | 40.078 | Ca | [Ca+2] | 1r9j |
| 154-87-0 | THIAMINE DIPHOSPHATE | 425.314 | C12 H19 N4 O7 P2 S | O=P(O)(O)OP(=O)(O)OCCc1sc[n+](c1C)Cc2c(nc(nc2)C)N | 1r9j |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/169013/Q4QAC4.pdb 2.0 671 = residues | | = | *| Ramachandran plot: 90.6% core 9.1% allow 0.2% gener 0.2% = disall | | = | +| All Ramachandrans: 6 labelled residues (out of 669) = | +| Chi1-chi2 plots: 4 labelled residues (out of 337) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 2 | *| Bond len/angle: 6.5 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.17 Covalent: -0.07 Overall: = 0.08 | | = | | M/c bond lengths: 99.6% within limits 0.4% highlighted = | | M/c bond angles: 95.5% within limits 4.5% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |