| Serine/threonine protein phosphatase, putative [Q4Q9W1] | |
|---|---|
| Systematic Name | LmjF.25.1320 [Leishmania major] |
| Gene Name | LMJF_25_1320 |
| Molecular Weight | 35861 Da |
| Protein Sequence Size | 317 |
| Function | |
| Charge | -6 |
| Isoelectric Point | 5.5248 pH |
| Description | Serine/threonine protein phosphatase, putative (EC 3.1.3.16). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 3.1.3.16 |
| Sequence | >tr|Q4Q9W1|Q4Q9W1_LEIMA Serine/threonine protein phosphatase, putative (EC 3.1.3.16) - Leishmania major TTAGGGSAVGSSSALDLDEMINYVIQCKPLSEQQVARLCEKVKEVLEKENNVHAVRAPVT VCGDVHGQFHDLLELFKIGGLPPDTNYLFMGDYVDRGYYSVETVTLLLLYKLRYPQRLHL LRGNHESRQITQVYGFYDECIRKYGSANVWTIFTDLFDYLPLTALVENDIFCLHGGLSPT VDTFSHIRNLDRVQEVPHEGPMCDLLWSDPDDRDGWGISPRGAGFTFGQGVTEGFCHNNK IKTIARAHQLVMDGYSWTHQDQLVTIFSAPNYCYRCGNLAGLLELDEHMNKCFFQFDPAP RRGEAQVSKKTPDYFL |
| DNA Sequence | >LmjF25.1320 |||serine/threonine protein phosphatase, putative|Leishmania major|chr 25|||Manual ATGACGACGG CCGGCGGCGG ATCGGCGGTG GGGTCCAGCA GCGCCCTCGA CTTGGATGAGATGATTAATT ACGTCATACA GTGCAAGCCG CTCAGCGAAC AGCAGGTCGC GCGCCTGTGCGAGAAGGTGA AGGAGGTGCT GGAGAAGGAG AACAACGTGC ACGCGGTGAG GGCGCCAGTCACGGTGTGCG GCGATGTGCA TGGCCAGTTT CACGACTTGC TGGAGCTTTT CAAGATTGGCGGGTTGCCGC CGGACACGAA CTACCTGTTC ATGGGGGACT ACGTGGACCG CGGCTACTACAGCGTCGAGA CGGTGACGCT GCTCCTCCTC TACAAGCTGC GATACCCCCA GCGACTTCATCTTCTCCGCG GCAACCACGA GTCGCGCCAG ATCACGCAGG TGTATGGCTT CTACGATGAGTGCATTCGCA AGTATGGTAG CGCCAACGTC TGGACGATCT TCACGGACCT GTTTGACTACCTGCCCCTGA CAGCGTTGGT CGAGAACGAC ATCTTCTGCC TTCACGGTGG TCTCTCACCGACGGTCGACA CATTCAGCCA CATCCGCAAC CTCGACCGCG TGCAGGAAGT GCCGCACGAGGGGCCGATGT GCGATCTGCT ATGGTCGGAC CCGGACGATC GTGATGGCTG GGGCATCAGCCCCCGTGGCG CCGGCTTCAC CTTCGGCCAA GGAGTCACCG AAGGCTTCTG CCACAACAACAAGATCAAGA CAATCGCCCG TGCGCATCAG CTTGTCATGG ACGGCTACAG CTGGACGCATCAGGATCAGC TCGTCACGAT CTTCAGCGCA CCAAACTACT GCTACCGCTG CGGCAACCTCGCCGGTCTTT TGGAGCTTGA TGAGCACATG AACAAGTGCT TCTTTCAGTT CGACCCGGCGCCGCGACGCG GTGAAGCGCA GGTGTCGAAG AAGACGCCGG ACTATTTTCT ATAG |
| Serine/threonine protein phosphatase, putative Q4Q9W1] | |
|---|---|
| Metabolite Information | |
| Molecular Function | hydrolase activity; protein serine/threonine phosphatase activity |
| Biochemical Pathway | protein amino acid dephosphorylation |
| Regulatory Pathway | |
| KEGG Pathways | K04382 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | protein phosphatase 2, catalytic subunit, beta isoform [Homo sapiens] | 69 | 1e-130 | 463 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YER133w serthr phosphoprotein phosphatase 1, catalytic chain | Saccharomyces cerevisiae | 44% | 1e-74 | 274 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00115 | Serine/threonine specific protein phosphatases signature | 122-127; | PS00125 | |
| Acylation | N-myristoylation site | 5-10; 7-12; 11-16; 124-129; 177-182; 223-228; 229-234; 231-236; 278-283; | PS00008 | |
| Phosphorylation | Casein kinase II phosphorylation site | 14-17; 209-212; 259-262; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 220-222; 309-311; | PS00005 | |
| Serine/threonine protein phosphatase, putative [Q4Q9W1] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2nppF | |
| Percent Identity | 69% | |
| Target Region | 1-317 | |
| Template Region | 2-308 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 96 | Sidechain |
| ARG | 97 | Sidechain |
| ASN | 125 | Sidechain |
| HIS | 126 | Sidechain |
| ARG | 222 | Sidechain |
| HIS | 249 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 16397-91-4 | MANGANESE (II) ION | 54.938 | Mn | [Mn+2] | 2npp |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/3914199/Q4Q9W1.pdb 2.0 317 = residues | | = | *| Ramachandran plot: 86.8% core 11.0% allow 1.5% gener 0.7% = disall | | = | +| All Ramachandrans: 7 labelled residues (out of 315) = | +| Chi1-chi2 plots: 2 labelled residues (out of 194) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.6 Bad contacts: = 4 | *| Bond len/angle: 7.2 Morris et al class: 1 = 1 2 | +| 2 cis-peptides = | | G-factors Dihedrals: 0.00 Covalent: -0.12 Overall: = -0.04 | | = | | M/c bond lengths: 99.6% within limits 0.4% highlighted = | | M/c bond angles: 94.0% within limits 6.0% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |