| Pyruvate dehydrogenase E1 beta subunit, putative [Q4Q9R8] | |
|---|---|
| Systematic Name | LmjF.25.1710 [Leishmania major] |
| Gene Name | LMJF_25_1710 |
| Molecular Weight | 37898 Da |
| Protein Sequence Size | 350 |
| Function | |
| Charge | -3 |
| Isoelectric Point | 5.716 pH |
| Description | Pyruvate dehydrogenase E1 beta subunit, putative (EC 1.2.4.1). |
| Subcellular Location | Mitochondrion[Predict] |
| E. C. Number | 1.2.4.1 |
| Sequence | >tr|Q4Q9R8|Q4Q9R8_LEIMA Pyruvate dehydrogenase E1 beta subunit, putative (EC 1.2.4.1) - Leishmania major RRFASRALFSASAAMAARCATTNMTVRDAIHSALDEELAREEKVFVIGEEVAQYQGAYKV TKGLMDKYGKDRIIDMPITEHGFAGMAVGAALSGLRPVCEFMTFNFAMQAIDQLVNSAGK SLYMSGGQMKCPIVFRGPNGASAGVGAQHSQCFGPWYASVPGLKVIAPYNCEDARGMIKA AIRDDNAVVVLEHELLYSESFPVTDEAADKNFVIPFGKAKIEREGKDITLIGFSRGVDLC LKAAEKLAAEGVQAEVINLRSLRPLDRHTILSSIKKTHRAVTVDESFPVCNIGAEICACV MESDTFDYLDAPIERVSCADCPTPYSKDIEMASQPQVADVMAAAKRVLS |
| DNA Sequence | >LmjF25.1710 |||pyruvate dehydrogenase E1 beta subunit, putative|Leishmania major|chr 25|||Manual ATGCGCCGCT TCGCCTCCCG AGCTCTCTTC TCCGCGTCCG CCGCCATGGC GGCGCGCTGCGCCACGACCA ACATGACGGT GCGCGATGCG ATCCACTCGG CCCTCGACGA GGAGCTCGCCCGCGAAGAGA AGGTCTTTGT GATTGGTGAG GAGGTCGCCC AATACCAGGG AGCCTACAAGGTGACCAAGG GTCTTATGGA CAAGTATGGC AAGGACCGCA TCATCGACAT GCCCATCACAGAGCACGGCT TCGCCGGCAT GGCCGTTGGT GCCGCTCTCA GTGGCCTGCG CCCCGTGTGCGAGTTCATGA CCTTCAACTT CGCCATGCAA GCTATCGATC AGCTTGTCAA CTCAGCCGGCAAGAGCCTGT ATATGTCTGG TGGCCAGATG AAGTGCCCCA TAGTCTTTCG TGGTCCCAACGGTGCGTCGG CTGGTGTAGG TGCCCAGCAT AGTCAGTGCT TCGGTCCGTG GTACGCGTCCGTGCCCGGCC TGAAGGTAAT TGCGCCGTAC AACTGCGAGG ATGCCCGCGG CATGATCAAGGCGGCCATCC GCGACGACAA CGCCGTGGTG GTGCTCGAGC ACGAGTTGCT GTACAGCGAGTCCTTCCCCG TCACAGACGA AGCAGCGGAC AAGAACTTTG TGATCCCGTT CGGCAAGGCGAAGATTGAGC GCGAGGGCAA GGACATCACC CTCATCGGCT TCTCTCGTGG TGTGGATCTGTGCTTGAAGG CGGCTGAGAA GCTCGCCGCA GAGGGCGTGC AGGCGGAGGT GATTAACCTTCGCTCCCTGC GCCCGCTCGA CCGCCACACC ATCCTCAGCT CCATCAAGAA GACGCACCGTGCGGTCACAG TCGATGAGTC CTTCCCTGTG TGCAACATTG GTGCTGAGAT CTGCGCCTGTGTCATGGAGA GCGACACGTT CGACTACCTC GATGCCCCGA TCGAGCGCGT GTCGTGCGCCGACTGCCCGA CACCGTACAG CAAGGATATC GAGATGGCTT CGCAGCCGCA GGTGGCCGACGTCATGGCCG CTGCCAAGCG CGTACTCAGC TAA |
| Pyruvate dehydrogenase E1 beta subunit, putative Q4Q9R8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | pyruvate dehydrogenase (acetyl-transferring) activity |
| Biochemical Pathway | metabolism |
| Regulatory Pathway | |
| KEGG Pathways | K00162 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | pyruvate dehydrogenase (lipoamide) beta [Homo sapiens] | 59 | 1e-110 | 397 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| bfmBAB branched-chain alpha-keto acid dehydrogenase E1 | Staphylococcus aureus | 34% | 3e-52 | 199 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| Acylation | N-myristoylation site | 86-91; 90-95; 141-146; 147-152; 177-182; 237-242; 294-299; | PS00008 | |
| Glycosylation | N-glycosylation site | 24-27; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 26-29; 33-36; 283-286; 318-321; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 26-28; 262-264; 274-276; 278-280; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 191-205; 302-316; | PS00003 | |
| Pyruvate dehydrogenase E1 beta subunit, putative [Q4Q9R8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 2ozlD | |
| Percent Identity | 59% | |
| Target Region | 22-350 | |
| Template Region | 1-330 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| GLU | 60 | Unknown |
| HIS | 129 | Unknown |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 24203-36-9 | POTASSIUM ION | 39.098 | K | [K+] | 2ozl |
| 7791-18-6 | MAGNESIUM ION | 24.305 | Mg | [Mg+2] | 2ozl |
| 154-87-0 | THIAMINE DIPHOSPHATE | 425.314 | C12 H19 N4 O7 P2 S | O=P(O)(O)OP(=O)(O)OCCc1sc[n+](c1C)Cc2c(nc(nc2)C)N | 2ozl |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/13600/Q4Q9R8.pdb 2.0 329 = residues | | = | *| Ramachandran plot: 92.7% core 6.2% allow 0.7% gener 0.3% = disall | | = | +| All Ramachandrans: 3 labelled residues (out of 327) = | +| Chi1-chi2 plots: 1 labelled residues (out of 180) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 0 | *| Bond len/angle: 5.0 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.14 Covalent: -0.06 Overall: = 0.07 | | = | | M/c bond lengths: 99.6% within limits 0.4% highlighted = | | M/c bond angles: 95.5% within limits 4.5% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |