| Adenine phosphoribosyltransferase [Q4Q9H8] | |
|---|---|
| Systematic Name | LmjF.26.0140 [Leishmania major] |
| Gene Name | APRT |
| Molecular Weight | 26255 Da |
| Protein Sequence Size | 237 |
| Function | |
| Charge | 1.5 |
| Isoelectric Point | 7.0303 pH |
| Description | Adenine phosphoribosyltransferase (EC 2.4.2.7). |
| Subcellular Location | Cytoplasm[Predict] |
| E. C. Number | 2.4.2.7 |
| Sequence | >tr|Q4Q9H8|Q4Q9H8_LEIMA Adenine phosphoribosyltransferase (EC 2.4.2.7) - Leishmania major SFKEISPNSFLLEDSHPLSQLLKKNYCWYSPVFSPRNVPRFADVSSITESPETLKAIRDF LVQRYRTMSPAPTHILGFDARGFLFGPMIAVELGIPFVLMRKADKNAGLLIRSEPYEKEY KEAAPEVMTIRYGSISKGSRVVLIDDVLATGGTALSGLQLVEASDAMVVEMVSILCIPFL KAAEKIHSTGHSRYKDIKFISLLSEEALTEDNCGDSKNYTGPRVVSCGDVLSKHSQ |
| DNA Sequence | >LmjF26.0140 |APRTASE||adenine phosphoribosyltransferase|Leishmania major|chr 26|||Manual ATGTCCTTCA AGGAAATAAG CCCAAACTCT TTCCTGCTAG AGGATTCCCA CCCACTCTCGCAGCTGCTAA AGAAGAACTA CTGCTGGTAC TCCCCAGTCT TCTCTCCGCG TAATGTCCCCCGCTTCGCCG ATGTCAGCAG CATCACCGAG TCTCCAGAGA CATTGAAAGC TATTCGTGACTTCCTTGTGC AACGGTACCG AACCATGTCG CCGGCCCCGA CGCATATCCT CGGCTTCGATGCTCGCGGCT TCCTCTTTGG CCCCATGATC GCCGTGGAGC TTGGGATCCC GTTCGTGTTGATGCGCAAGG CGGACAAGAA TGCCGGTCTC CTTATCCGCA GCGAACCGTA CGAAAAGGAGTACAAAGAGG CTGCGCCGGA GGTGATGACG ATCCGCTACG GCAGCATCAG CAAGGGATCGCGTGTGGTGC TCATCGACGA CGTCCTGGCA ACAGGTGGGA CGGCGCTGTC GGGCTTACAACTGGTGGAGG CAAGCGACGC AATGGTTGTT GAGATGGTCT CGATTTTGTG CATTCCTTTCCTGAAGGCGG CAGAGAAGAT CCACTCGACA GGTCATAGCC GCTACAAGGA TATTAAGTTTATTAGTCTTC TTTCCGAGGA GGCGCTGACG GAGGACAACT GTGGGGACTC AAAGAACTACACGGGTCCTC GTGTTGTGAG CTGCGGTGAT GTGCTATCCA AGCACTCTCA ATAG |
| Adenine phosphoribosyltransferase Q4Q9H8] | |
|---|---|
| Metabolite Information | |
| Molecular Function | adenine phosphoribosyltransferase activity |
| Biochemical Pathway | nucleoside metabolism; purine ribonucleoside salvage |
| Regulatory Pathway | |
| KEGG Pathways | K00759 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | adenine phosphoribosyltransferase isoform a [Homo sapiens] | 35 | 1e-17 | 87 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| apt adenine phosphoribosyltransferase | Haemophilus influenzae | 32% | 3e-20 | 92.4 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00096 | Purine/pyrimidine phosphoribosyl transferases signature | 142-154; | PS00103 | |
| Acylation | N-myristoylation site | 153-158; | PS00008 | |
| Glycosylation | N-glycosylation site | 219-222; | PS00001 | |
| Phosphorylation | Casein kinase II phosphorylation site | 2-5; 47-50; 227-230; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 2-4; 35-37; 54-56; 130-132; | PS00005 | |
| Adenine phosphoribosyltransferase [Q4Q9H8] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1qcdA | |
| Percent Identity | 92% | |
| Target Region | 1-237 | |
| Template Region | 2-236 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ARG | 82 | Sidechain |
| LYS | 106 | Sidechain |
| GLU | 120 | Sidechain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 14808-79-8 | SULFATE ION | 96.063 | O4 S   | [O-]S([O-])(=O)=O | 1qcd |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/126275/Q4Q9H8.pdb 2.0 230 = residues | | = | | Ramachandran plot: 94.4% core 5.6% allow 0.0% gener 0.0% = disall | | = | +| All Ramachandrans: 3 labelled residues (out of 224) = | +| Chi1-chi2 plots: 1 labelled residues (out of 130) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 16.1 Bad contacts: = 1 | *| Bond len/angle: 10.8 Morris et al class: 1 = 1 2 | +| 1 cis-peptides = | | G-factors Dihedrals: 0.13 Covalent: -0.10 Overall: = 0.04 | | = | | M/c bond lengths: 99.1% within limits 0.9% highlighted = | *| M/c bond angles: 95.5% within limits 4.5% highlighted 1 off = graph | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |