| Asparagine synthetase a, putative [Q4Q9B1] | |
|---|---|
| Systematic Name | LmjF.26.0830 [Leishmania major] |
| Gene Name | LMJF_26_0830 |
| Molecular Weight | 39805 Da |
| Protein Sequence Size | 353 |
| Function | |
| Charge | -6.5 |
| Isoelectric Point | 5.4216 pH |
| Description | Asparagine synthetase a, putative (Aspartate--ammonia ligase, putative) (EC 6.3.1.1). |
| Subcellular Location | cytoplasm[Predict] |
| E. C. Number | 6.3.1.1 |
| Sequence | >tr|Q4Q9B1|Q4Q9B1_LEIMA Asparagine synthetase a, putative (Aspartate--ammonia ligase, putative) (EC 6.3.1.1) - Leishmania major. SSDPQAYIDLQTRILRVKTIFAEELANALNLIQVEGPILARVGDGTQDNLSGVEKAVQVH VKEIPGENYEVVHSLAKWKRMTLGNHKFPVGRGIFTNMHALRVEETLDSIHSVYVDQWDW ERVIAPADRCLEYLQATVRSLYEVLRETERRLCTEFPDIAPILPDSITFVHAEQLLKRYP ELDPKSRELEAVKQFGAVFLIGIGCKLSHGDHHDARAPDYDDWSSPVSVDSSKIGFPTAD DEKPSVNTIMSLQGLNGDILVYNPVLNDALELSSMGIRVDQEALRRQLEITGTSDRLKCR WHQCVLNGDLPQTIGGGIGQSRTVMFMLRKRHIGEVQCSVWPYEVTTQYSLL |
| DNA Sequence | >LmjF26.0830 |||asparagine synthetase a, putative|Leishmania major|chr 26|||Manual ATGTCGTCCG ATCCGCAGGC GTACATTGAT CTCCAGACCC GCATTTTGAG GGTGAAAACGATCTTCGCCG AGGAGCTCGC CAATGCCTTG AACCTGATCC AGGTGGAGGG CCCCATACTCGCGCGCGTCG GCGATGGCAC ACAGGACAAC CTCTCGGGTG TGGAGAAGGC GGTGCAGGTGCACGTGAAAG AGATCCCGGG GGAGAATTAC GAGGTAGTGC ACTCGCTTGC CAAGTGGAAGCGCATGACCC TCGGCAATCA CAAGTTCCCC GTTGGCAGGG GCATCTTTAC CAACATGCACGCCCTGCGTG TCGAAGAAAC CCTGGATAGT ATCCACTCCG TCTACGTCGA CCAGTGGGACTGGGAGCGCG TGATAGCGCC GGCGGACCGC TGCCTCGAGT ACCTGCAGGC CACCGTTCGCTCTCTCTACG AGGTGCTGCG CGAGACGGAG AGGCGCTTGT GCACCGAGTT CCCGGACATTGCCCCGATTC TGCCGGATTC GATCACGTTC GTGCACGCCG AGCAGCTGCT GAAGCGCTACCCGGAGCTGG ACCCCAAGTC GCGCGAGCTG GAAGCCGTCA AGCAGTTCGG AGCGGTGTTCCTGATCGGCA TCGGGTGCAA GCTGAGCCAC GGCGACCACC ATGACGCGCG TGCTCCGGACTACGATGACT GGTCGTCGCC GGTGTCCGTC GACTCCTCCA AGATCGGCTT TCCGACTGCCGACGACGAGA AGCCCTCGGT GAATACTATC ATGTCCTTGC AGGGCCTTAA CGGCGACATCCTGGTGTACA ACCCGGTTCT CAATGATGCG CTCGAACTGA GCAGCATGGG CATCCGTGTTGACCAGGAGG CGCTGCGCCG CCAGCTGGAG ATCACCGGCA CCAGCGACCG CCTCAAGTGCAGGTGGCATC AGTGCGTGCT GAACGGAGAC CTACCTCAAA CCATCGGCGG CGGTATCGGCCAGAGCCGCA CAGTTATGTT CATGCTCCGA AAGAGGCACA TCGGCGAGGT GCAGTGCAGCGTGTGGCCCT ACGAGGTCAC GACGCAGTAC TCCTTATTGT AA |
| Asparagine synthetase a, putative Q4Q9B1] | |
|---|---|
| Metabolite Information | |
| Molecular Function | ATP binding; aminoacyl-tRNA ligase activity; aspartate-ammonia ligase activity |
| Biochemical Pathway | asparagine biosynthesis; tRNA aminoacylation for protein translation |
| Regulatory Pathway | |
| KEGG Pathways | K01914 |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | DnaJ (Hsp40) homolog, subfamily C, member 8 [Homo sapiens] | 23 | 4.2 | 30 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| asnA aspartate-ammonia ligase | Haemophilus influenzae | 53% | 3e-92 | 332 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC00363 | Aminoacyl-transfer RNA synthetases class-II profiles | 18.948 | 18-343 | PS50862 |
| Acylation | N-myristoylation site | 46-51; 53-58; 94-99; 205-210; 316-321; 318-323; 320-325; | PS00008 | |
| Glycosylation | N-glycosylation site | 50-53; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 80-83; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 52-55; 141-144; 209-212; 239-242; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 138-140; 149-151; 232-234; 295-297; | PS00005 | |
| Sulfation | Tyrosine sulfation site | 214-228; | PS00003 | |
| Asparagine synthetase a, putative [Q4Q9B1] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 12asA | |
| Percent Identity | 58% | |
| Target Region | 1-353 | |
| Template Region | 4-330 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
|---|---|---|
| Residue | Active Site Number | Functional Part |
| ASP | 49 | Sidechain |
| Asp | 50 | Side Chain |
| ARG | 103 | Sidechain |
| Arg | 104 | Side Chain |
| GLN | 118 | Sidechain |
| Gln | 119 | Side Chain |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| 61-19-8 | ADENOSINE MONOPHOSPHATE  | 347.221 | C10 H14 N5 O7 P   | O=P(O)(O)OCC3OC(n2cnc1c(ncnc12)N)C(O)C3O | 12as |
| 70-47-3 | ASPARAGINE | 132.118 | C4 H8 N2 O3   | O=C(N)CC(N)C(=O)O | 12as |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/3396030/Q4Q9B1.pdb 2.0 353 = residues | | = | +| Ramachandran plot: 92.6% core 6.8% allow 0.6% gener 0.0% = disall | | = | +| All Ramachandrans: 6 labelled residues (out of 351) = | +| Chi1-chi2 plots: 3 labelled residues (out of 216) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 3.2 Bad contacts: = 11 | *| Bond len/angle: 7.8 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: -0.03 Covalent: -0.19 Overall: = -0.08 | | = | | M/c bond lengths: 99.0% within limits 1.0% highlighted = | | M/c bond angles: 92.2% within limits 7.8% highlighted = | | Planar groups: 100.0% within limits 0.0% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |