| Nitrilase, putative [Q4Q8W4] | |
|---|---|
| Systematic Name | LmjF.26.2280 [Leishmania major] |
| Gene Name | LMJF_26_2280 |
| Molecular Weight | 30967 Da |
| Protein Sequence Size | 279 |
| Function | |
| Charge | -0.5 |
| Isoelectric Point | 6.3 pH |
| Description | Nitrilase, putative (EC 3.5.5.1). |
| Subcellular Location | N.A.[Predict] |
| E. C. Number | 3.5.5.1 |
| Sequence | >tr|Q4Q8W4|Q4Q8W4_LEIMA Nitrilase, putative (EC 3.5.5.1) - Leishmania major. ASVLPVTLCQMAVTREKAANIKKAVTMITEAAKRGSKLAVLPECFNCPYGTKYFDEYSEA LAPGNETFDAMSQCAKANSIWIVAGSIPEKSADGKLFNSSMTFGSDGALKHVHRKVHLFC INTDTVRFDESEVLSAGNDATAISLDEHTKFGVAICFDIRYPFLAWKYAEQGTSFIVYPG AFNMVTGPMHWQLAARARAVDNQQYVFVCSPARDTSAEYVAWGHSMVVDPIGNVLSELDE KEGFVDWKVDLSVIQDTRNRIPILKGVRDDLYTLHWKK |
| DNA Sequence | >LmjF26.2280 |||nitrilase, putative|Leishmania major|chr 26|||Manual ATGGCGTCCG TTCTGCCCGT GACTCTTTGC CAAATGGCTG TCACGCGTGA AAAGGCAGCCAATATTAAAA AGGCTGTCAC AATGATCACT GAAGCCGCGA AGCGGGGCAG CAAGCTGGCTGTCTTACCCG AGTGCTTCAA CTGCCCGTAT GGTACCAAGT ATTTCGACGA GTACTCTGAGGCCCTTGCGC CAGGCAACGA AACATTTGAT GCGATGTCCC AGTGCGCCAA GGCGAACAGCATCTGGATCG TTGCAGGTAG CATTCCCGAA AAGTCAGCTG ACGGAAAACT GTTCAACTCGAGCATGACGT TTGGATCGGA TGGTGCGCTG AAACACGTAC ACCGCAAGGT GCATCTGTTCTGCATCAACA CGGATACCGT TCGCTTCGAC GAGAGCGAAG TGCTCAGTGC TGGAAACGATGCCACTGCCA TTTCGCTGGA CGAACACACA AAGTTTGGCG TTGCTATTTG CTTCGACATCCGATACCCCT TCCTGGCGTG GAAATACGCT GAACAAGGAA CCTCCTTTAT TGTGTACCCCGGCGCCTTTA ACATGGTGAC CGGCCCGATG CACTGGCAGC TGGCAGCGCG CGCTCGCGCTGTGGACAATC AGCAGTATGT TTTCGTGTGT TCCCCTGCCC GCGACACCTC CGCGGAGTACGTGGCATGGG GGCACTCCAT GGTGGTAGAT CCCATTGGAA ATGTTCTCTC GGAGCTGGATGAGAAGGAGG GGTTTGTTGA TTGGAAGGTA GACCTCAGCG TCATTCAAGA TACGCGCAACCGAATTCCCA TCCTGAAAGG CGTGCGTGAC GACCTCTACA CCCTGCATTG GAAGAAGTAG |
| Nitrilase, putative Q4Q8W4] | |
|---|---|
| Metabolite Information | |
| Molecular Function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds |
| Biochemical Pathway | nitrogen compound metabolism |
| Regulatory Pathway | |
| KEGG Pathways | |
| Orthologs | ||||
| Homologs | GI | Percent Identity | Evalue | Score |
| Homo sapiens | nitrilase family, member 2 [Homo sapiens] | 45 | 2e-64 | 242 |
| DEG Information | ||||
| DEG Protein | DEG Organism | Percent Identity | Evalue | Bit Score |
| YLR116w branch point bridging protein | Saccharomyces cerevisiae | 27% | 3.8 | 26.2 |
| Post Translational Modification | ||||
| PTM Type | PTM Sub Type | Score | Modification Site | Prosite ID |
| PDOC50263 | Carbon-nitrogen hydrolase domain profile | 43.61 | 5-274 | PS50263 |
| Acylation | N-myristoylation site | 36-41; 105-110; 138-143; 153-158; 233-238; | PS00008 | |
| Glycosylation | N-glycosylation site | 66-69; 99-102; | PS00001 | |
| Phosphorylation | cAMP- and cGMP-dependent protein kinase phosphorylation site | 34-37; | PS00004 | |
| Phosphorylation | Casein kinase II phosphorylation site | 87-90; 145-148; 216-219; 237-240; | PS00006 | |
| Phosphorylation | Protein kinase C phosphorylation site | 126-128; | PS00005 | |
| Phosphorylation | Tyrosine kinase phosphorylation site | 199-206; 266-273; | PS00007 | |
| Sulfation | Tyrosine sulfation site | 51-65; | PS00003 | |
| Nitrilase, putative [Q4Q8W4] | ||
|---|---|---|
| Model Information | ||
| Template PDB ID | 1f89B | |
| Percent Identity | 43% | |
| Target Region | 1-269 | |
| Template Region | 302-581 | |
| Domain Information | ||
|---|---|---|
| Domains | Start | End |
| Active Site Information | ||
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| Residue | Active Site Number | Functional Part |
| Co-Factor | |
|---|---|
| Metal | Description |
| Ligands | |||||
|---|---|---|---|---|---|
| CAS number | Name | Mol. Weight | Mol. Formula | Smile Notation | PDB Reference |
| Mutational Information | ||
|---|---|---|
| Residue | Feature | Description |
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| Modeled Protein | Template Structure |
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+----------<<< P R O C H E C K S U M = M A R Y >>>----------+ | = | | /var/www/html/Services/SAVES_3/jobs/331981/Q4Q8W4.pdb 2.0 269 = residues | | = | *| Ramachandran plot: 87.5% core 10.8% allow 0.8% gener 0.8% = disall | | = | *| All Ramachandrans: 9 labelled residues (out of 267) = | +| Chi1-chi2 plots: 2 labelled residues (out of 147) = | | = | | Main-chain params: 6 better 0 inside 0 worse = | | Side-chain params: 5 better 0 inside 0 worse = | | = | *| Residue properties: Max.deviation: 4.0 Bad contacts: = 6 | *| Bond len/angle: 8.9 Morris et al class: 1 = 1 2 | | = | | G-factors Dihedrals: 0.00 Covalent: -0.16 Overall: = -0.06 | | = | | M/c bond lengths: 99.3% within limits 0.7% highlighted = | *| M/c bond angles: 94.4% within limits 5.6% highlighted 1 off = graph | +| Planar groups: 98.9% within limits 1.1% highlighted = | | = | = +------------------------------------------------------------------------= ----+ + May be worth investigating further. * Worth investigating further. |